Data_Sheet_1_Exploratory screening for micro-RNA biomarkers in canine multicentric lymphoma.pdf
Lymphoma is one of the most frequent hematopoietic tumors in dogs and shares similar features with human counterparts. MicroRNAs (miRNA, small non-coding RNAs) are pivotal in gene regulation fine tuning and cancer hallmarks are influenced by their aberrant expression. Consequently, miRNA biomarkers...
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ftfrontimediafig:oai:figshare.com:article/25848298 2024-09-15T18:01:29+00:00 Data_Sheet_1_Exploratory screening for micro-RNA biomarkers in canine multicentric lymphoma.pdf Sabine E. Hammer Julia Sprung Ondřej Škor Stefanie Burger Martin Hofer Ilse Schwendenwein Barbara C. Rütgen 2024-05-17T13:38:37Z https://doi.org/10.3389/fvets.2024.1379146.s001 https://figshare.com/articles/dataset/Data_Sheet_1_Exploratory_screening_for_micro-RNA_biomarkers_in_canine_multicentric_lymphoma_pdf/25848298 unknown doi:10.3389/fvets.2024.1379146.s001 https://figshare.com/articles/dataset/Data_Sheet_1_Exploratory_screening_for_micro-RNA_biomarkers_in_canine_multicentric_lymphoma_pdf/25848298 CC BY 4.0 Animal Systematics and Taxonomy Animal Physiology - Biophysics Animal Physiology - Cell Animal Physiology - Systems Animal Behaviour Animal Cell and Molecular Biology Animal Developmental and Reproductive Biology Animal Immunology Animal Neurobiology Animal Physiological Ecology Animal Structure and Function Veterinary Anaesthesiology and Intensive Care Veterinary Anatomy and Physiology Veterinary Diagnosis and Diagnostics Veterinary Epidemiology Veterinary Immunology Veterinary Medicine Veterinary Microbiology (excl. Virology) Veterinary Parasitology Veterinary Pathology Veterinary Pharmacology Veterinary Surgery Veterinary Virology Veterinary Sciences not elsewhere classified Canis lupus familiaris diffuse large B-cell lymphoma (DLBCL) peripheral T-cell lymphoma (PTCL) microRNA expression analysis potential biomarker candidates animal model biomedical research and development Dataset 2024 ftfrontimediafig https://doi.org/10.3389/fvets.2024.1379146.s001 2024-08-19T06:19:44Z Lymphoma is one of the most frequent hematopoietic tumors in dogs and shares similar features with human counterparts. MicroRNAs (miRNA, small non-coding RNAs) are pivotal in gene regulation fine tuning and cancer hallmarks are influenced by their aberrant expression. Consequently, miRNA biomarkers may assist predicting therapeutic response and clinical outcome by providing less-invasive novel diagnostics tools. The aim of this study was to detect dysregulated miRNAs in lymphomatous lymph node tissues in comparison to lymph node material or PBMCs from healthy control dogs. Potential significant differences in miRNA expression profiles between four lymphoma entities were evaluated. A customized PCR array was utilized to profile 89 canine target miRNAs. Quantification was performed using qPCR, relative expression was determined by the delta–delta Ct method, and p-values were calculated with student’s t-test. In the 14 diffuse large B-cell lymphoma (DLBCL) patients, 28 and 24 different miRNAs were significantly dysregulated compared to lymph node material or PBMCs. Sixteen miRNAs occurred in both control groups, with 12 miRNAs being down- and four miRNAs being upregulated. The six peripheral T-cell lymphoma (PTCL) samples showed 24 and 25 dysregulated miRNAs when compared to the healthy controls. A combined analysis of DLBCL and PTCL samples revealed seven shared and 19 differently expressed miRNAs. Potential biomarkers in T- and B-cell lymphoma could be the miRNA-17/92 cluster and miRNA-181-family together with miRNA-34a and miRNA-150. Diagnostic utility of potential biomarkers must be validated in larger, prospective cohorts of canine lymphoma cases and in higher numbers of physiological patient material. Dataset Canis lupus Frontiers: Figshare |
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Frontiers: Figshare |
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ftfrontimediafig |
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topic |
Animal Systematics and Taxonomy Animal Physiology - Biophysics Animal Physiology - Cell Animal Physiology - Systems Animal Behaviour Animal Cell and Molecular Biology Animal Developmental and Reproductive Biology Animal Immunology Animal Neurobiology Animal Physiological Ecology Animal Structure and Function Veterinary Anaesthesiology and Intensive Care Veterinary Anatomy and Physiology Veterinary Diagnosis and Diagnostics Veterinary Epidemiology Veterinary Immunology Veterinary Medicine Veterinary Microbiology (excl. Virology) Veterinary Parasitology Veterinary Pathology Veterinary Pharmacology Veterinary Surgery Veterinary Virology Veterinary Sciences not elsewhere classified Canis lupus familiaris diffuse large B-cell lymphoma (DLBCL) peripheral T-cell lymphoma (PTCL) microRNA expression analysis potential biomarker candidates animal model biomedical research and development |
spellingShingle |
Animal Systematics and Taxonomy Animal Physiology - Biophysics Animal Physiology - Cell Animal Physiology - Systems Animal Behaviour Animal Cell and Molecular Biology Animal Developmental and Reproductive Biology Animal Immunology Animal Neurobiology Animal Physiological Ecology Animal Structure and Function Veterinary Anaesthesiology and Intensive Care Veterinary Anatomy and Physiology Veterinary Diagnosis and Diagnostics Veterinary Epidemiology Veterinary Immunology Veterinary Medicine Veterinary Microbiology (excl. Virology) Veterinary Parasitology Veterinary Pathology Veterinary Pharmacology Veterinary Surgery Veterinary Virology Veterinary Sciences not elsewhere classified Canis lupus familiaris diffuse large B-cell lymphoma (DLBCL) peripheral T-cell lymphoma (PTCL) microRNA expression analysis potential biomarker candidates animal model biomedical research and development Sabine E. Hammer Julia Sprung Ondřej Škor Stefanie Burger Martin Hofer Ilse Schwendenwein Barbara C. Rütgen Data_Sheet_1_Exploratory screening for micro-RNA biomarkers in canine multicentric lymphoma.pdf |
topic_facet |
Animal Systematics and Taxonomy Animal Physiology - Biophysics Animal Physiology - Cell Animal Physiology - Systems Animal Behaviour Animal Cell and Molecular Biology Animal Developmental and Reproductive Biology Animal Immunology Animal Neurobiology Animal Physiological Ecology Animal Structure and Function Veterinary Anaesthesiology and Intensive Care Veterinary Anatomy and Physiology Veterinary Diagnosis and Diagnostics Veterinary Epidemiology Veterinary Immunology Veterinary Medicine Veterinary Microbiology (excl. Virology) Veterinary Parasitology Veterinary Pathology Veterinary Pharmacology Veterinary Surgery Veterinary Virology Veterinary Sciences not elsewhere classified Canis lupus familiaris diffuse large B-cell lymphoma (DLBCL) peripheral T-cell lymphoma (PTCL) microRNA expression analysis potential biomarker candidates animal model biomedical research and development |
description |
Lymphoma is one of the most frequent hematopoietic tumors in dogs and shares similar features with human counterparts. MicroRNAs (miRNA, small non-coding RNAs) are pivotal in gene regulation fine tuning and cancer hallmarks are influenced by their aberrant expression. Consequently, miRNA biomarkers may assist predicting therapeutic response and clinical outcome by providing less-invasive novel diagnostics tools. The aim of this study was to detect dysregulated miRNAs in lymphomatous lymph node tissues in comparison to lymph node material or PBMCs from healthy control dogs. Potential significant differences in miRNA expression profiles between four lymphoma entities were evaluated. A customized PCR array was utilized to profile 89 canine target miRNAs. Quantification was performed using qPCR, relative expression was determined by the delta–delta Ct method, and p-values were calculated with student’s t-test. In the 14 diffuse large B-cell lymphoma (DLBCL) patients, 28 and 24 different miRNAs were significantly dysregulated compared to lymph node material or PBMCs. Sixteen miRNAs occurred in both control groups, with 12 miRNAs being down- and four miRNAs being upregulated. The six peripheral T-cell lymphoma (PTCL) samples showed 24 and 25 dysregulated miRNAs when compared to the healthy controls. A combined analysis of DLBCL and PTCL samples revealed seven shared and 19 differently expressed miRNAs. Potential biomarkers in T- and B-cell lymphoma could be the miRNA-17/92 cluster and miRNA-181-family together with miRNA-34a and miRNA-150. Diagnostic utility of potential biomarkers must be validated in larger, prospective cohorts of canine lymphoma cases and in higher numbers of physiological patient material. |
format |
Dataset |
author |
Sabine E. Hammer Julia Sprung Ondřej Škor Stefanie Burger Martin Hofer Ilse Schwendenwein Barbara C. Rütgen |
author_facet |
Sabine E. Hammer Julia Sprung Ondřej Škor Stefanie Burger Martin Hofer Ilse Schwendenwein Barbara C. Rütgen |
author_sort |
Sabine E. Hammer |
title |
Data_Sheet_1_Exploratory screening for micro-RNA biomarkers in canine multicentric lymphoma.pdf |
title_short |
Data_Sheet_1_Exploratory screening for micro-RNA biomarkers in canine multicentric lymphoma.pdf |
title_full |
Data_Sheet_1_Exploratory screening for micro-RNA biomarkers in canine multicentric lymphoma.pdf |
title_fullStr |
Data_Sheet_1_Exploratory screening for micro-RNA biomarkers in canine multicentric lymphoma.pdf |
title_full_unstemmed |
Data_Sheet_1_Exploratory screening for micro-RNA biomarkers in canine multicentric lymphoma.pdf |
title_sort |
data_sheet_1_exploratory screening for micro-rna biomarkers in canine multicentric lymphoma.pdf |
publishDate |
2024 |
url |
https://doi.org/10.3389/fvets.2024.1379146.s001 https://figshare.com/articles/dataset/Data_Sheet_1_Exploratory_screening_for_micro-RNA_biomarkers_in_canine_multicentric_lymphoma_pdf/25848298 |
genre |
Canis lupus |
genre_facet |
Canis lupus |
op_relation |
doi:10.3389/fvets.2024.1379146.s001 https://figshare.com/articles/dataset/Data_Sheet_1_Exploratory_screening_for_micro-RNA_biomarkers_in_canine_multicentric_lymphoma_pdf/25848298 |
op_rights |
CC BY 4.0 |
op_doi |
https://doi.org/10.3389/fvets.2024.1379146.s001 |
_version_ |
1810438629178212352 |