DataSheet1_Designing payload and spaceflight operations for plants from extreme terrestrial environments.docx
Terrestrial plants from the very limits of life are likely to harbor genes that confer an advantage in human space exploration. These plants are seemingly capable of performing mission critical functions in spaceflight and on extraterrestrial farms while informing directed gene manipulation in targe...
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ftfrontimediafig:oai:figshare.com:article/25494967 2024-09-15T17:48:46+00:00 DataSheet1_Designing payload and spaceflight operations for plants from extreme terrestrial environments.docx Agata K. Zupanska Emily Lockwood Ye Zhang Natasha J. Haveman John A. Carver Charles W. Spern Emily Senyk Jeffrey T. Richards Lawrence L. Koss Dinah I. Dimapilis Stuart F. McDaniel 2024-03-28T04:35:14Z https://doi.org/10.3389/frspt.2024.1376163.s001 https://figshare.com/articles/dataset/DataSheet1_Designing_payload_and_spaceflight_operations_for_plants_from_extreme_terrestrial_environments_docx/25494967 unknown doi:10.3389/frspt.2024.1376163.s001 https://figshare.com/articles/dataset/DataSheet1_Designing_payload_and_spaceflight_operations_for_plants_from_extreme_terrestrial_environments_docx/25494967 CC BY 4.0 Space Science Astronomical and Space Instrumentation Satellite Communications extreme environments plants Ceratodon spaceflight microgravity ISS chemical fixation deep-freeze Dataset 2024 ftfrontimediafig https://doi.org/10.3389/frspt.2024.1376163.s001 2024-08-19T06:19:46Z Terrestrial plants from the very limits of life are likely to harbor genes that confer an advantage in human space exploration. These plants are seemingly capable of performing mission critical functions in spaceflight and on extraterrestrial farms while informing directed gene manipulation in target plant species. However, their adaptations to physiologically extreme habitats may hinder the efficacy of routine laboratory techniques for model plants. We here present the development of Antarctic moss Ceratodon purpureus payload and flight operations for the ANT1 Radiation Tolerance Experiment with Moss in Orbit on the Space Station (ARTEMOSS) experiment to the International Space Station (ISS) given limited physical space and crew time. We demonstrate that the hydrophobic surface of Antarctic moss impedes chemical tissue fixation and precludes the use of RNAlater coupled with payload hardware deployed in standard plant spaceflight experiments. We show that deep-freezing the moss tissue on Petri plates provides adequate tissue fixation and allows the extraction of high-quality RNA suitable for gene expression profiling. We replaced hardware with stacks of Petri plates housing Antarctic moss and chemical fixation with deep-freezing in a cryogenic GLACIER freezer. Our design can be translated to other plant species to expand current experimentation techniques with plants from extreme terrestrial environments in order to advance human space exploration. Dataset Antarc* Antarctic Frontiers: Figshare |
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Frontiers: Figshare |
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ftfrontimediafig |
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Space Science Astronomical and Space Instrumentation Satellite Communications extreme environments plants Ceratodon spaceflight microgravity ISS chemical fixation deep-freeze |
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Space Science Astronomical and Space Instrumentation Satellite Communications extreme environments plants Ceratodon spaceflight microgravity ISS chemical fixation deep-freeze Agata K. Zupanska Emily Lockwood Ye Zhang Natasha J. Haveman John A. Carver Charles W. Spern Emily Senyk Jeffrey T. Richards Lawrence L. Koss Dinah I. Dimapilis Stuart F. McDaniel DataSheet1_Designing payload and spaceflight operations for plants from extreme terrestrial environments.docx |
topic_facet |
Space Science Astronomical and Space Instrumentation Satellite Communications extreme environments plants Ceratodon spaceflight microgravity ISS chemical fixation deep-freeze |
description |
Terrestrial plants from the very limits of life are likely to harbor genes that confer an advantage in human space exploration. These plants are seemingly capable of performing mission critical functions in spaceflight and on extraterrestrial farms while informing directed gene manipulation in target plant species. However, their adaptations to physiologically extreme habitats may hinder the efficacy of routine laboratory techniques for model plants. We here present the development of Antarctic moss Ceratodon purpureus payload and flight operations for the ANT1 Radiation Tolerance Experiment with Moss in Orbit on the Space Station (ARTEMOSS) experiment to the International Space Station (ISS) given limited physical space and crew time. We demonstrate that the hydrophobic surface of Antarctic moss impedes chemical tissue fixation and precludes the use of RNAlater coupled with payload hardware deployed in standard plant spaceflight experiments. We show that deep-freezing the moss tissue on Petri plates provides adequate tissue fixation and allows the extraction of high-quality RNA suitable for gene expression profiling. We replaced hardware with stacks of Petri plates housing Antarctic moss and chemical fixation with deep-freezing in a cryogenic GLACIER freezer. Our design can be translated to other plant species to expand current experimentation techniques with plants from extreme terrestrial environments in order to advance human space exploration. |
format |
Dataset |
author |
Agata K. Zupanska Emily Lockwood Ye Zhang Natasha J. Haveman John A. Carver Charles W. Spern Emily Senyk Jeffrey T. Richards Lawrence L. Koss Dinah I. Dimapilis Stuart F. McDaniel |
author_facet |
Agata K. Zupanska Emily Lockwood Ye Zhang Natasha J. Haveman John A. Carver Charles W. Spern Emily Senyk Jeffrey T. Richards Lawrence L. Koss Dinah I. Dimapilis Stuart F. McDaniel |
author_sort |
Agata K. Zupanska |
title |
DataSheet1_Designing payload and spaceflight operations for plants from extreme terrestrial environments.docx |
title_short |
DataSheet1_Designing payload and spaceflight operations for plants from extreme terrestrial environments.docx |
title_full |
DataSheet1_Designing payload and spaceflight operations for plants from extreme terrestrial environments.docx |
title_fullStr |
DataSheet1_Designing payload and spaceflight operations for plants from extreme terrestrial environments.docx |
title_full_unstemmed |
DataSheet1_Designing payload and spaceflight operations for plants from extreme terrestrial environments.docx |
title_sort |
datasheet1_designing payload and spaceflight operations for plants from extreme terrestrial environments.docx |
publishDate |
2024 |
url |
https://doi.org/10.3389/frspt.2024.1376163.s001 https://figshare.com/articles/dataset/DataSheet1_Designing_payload_and_spaceflight_operations_for_plants_from_extreme_terrestrial_environments_docx/25494967 |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_relation |
doi:10.3389/frspt.2024.1376163.s001 https://figshare.com/articles/dataset/DataSheet1_Designing_payload_and_spaceflight_operations_for_plants_from_extreme_terrestrial_environments_docx/25494967 |
op_rights |
CC BY 4.0 |
op_doi |
https://doi.org/10.3389/frspt.2024.1376163.s001 |
_version_ |
1810290285071040512 |