Image_1_Examining the impacts of elevated, variable pCO2 on larval Pacific razor clams (Siliqua patula) in Alaska.pdf

An increase in anthropogenic carbon dioxide is driving oceanic chemical shifts resulting in a long-term global decrease in ocean pH, colloquially termed ocean acidification (OA). Previous studies have demonstrated that OA can have negative physiological consequences for calcifying organisms, especia...

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Main Authors: Marina W. Alcantar, Jeff Hetrick, Jacqueline Ramsay, Amanda L. Kelley
Format: Still Image
Language:unknown
Published: 2024
Subjects:
Online Access:https://doi.org/10.3389/fmars.2024.1253702.s001
https://figshare.com/articles/figure/Image_1_Examining_the_impacts_of_elevated_variable_pCO2_on_larval_Pacific_razor_clams_Siliqua_patula_in_Alaska_pdf/25052108
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spelling ftfrontimediafig:oai:figshare.com:article/25052108 2024-09-15T18:28:00+00:00 Image_1_Examining the impacts of elevated, variable pCO2 on larval Pacific razor clams (Siliqua patula) in Alaska.pdf Marina W. Alcantar Jeff Hetrick Jacqueline Ramsay Amanda L. Kelley 2024-01-24T04:28:37Z https://doi.org/10.3389/fmars.2024.1253702.s001 https://figshare.com/articles/figure/Image_1_Examining_the_impacts_of_elevated_variable_pCO2_on_larval_Pacific_razor_clams_Siliqua_patula_in_Alaska_pdf/25052108 unknown doi:10.3389/fmars.2024.1253702.s001 https://figshare.com/articles/figure/Image_1_Examining_the_impacts_of_elevated_variable_pCO2_on_larval_Pacific_razor_clams_Siliqua_patula_in_Alaska_pdf/25052108 CC BY 4.0 Oceanography Marine Biology Marine Geoscience Biological Oceanography Chemical Oceanography Physical Oceanography Marine Engineering ocean acidification vaterite biomineralogy Siliqua patula variability larval response amorphous calcium carbonate (ACC) Image Figure 2024 ftfrontimediafig https://doi.org/10.3389/fmars.2024.1253702.s001 2024-08-19T06:19:47Z An increase in anthropogenic carbon dioxide is driving oceanic chemical shifts resulting in a long-term global decrease in ocean pH, colloquially termed ocean acidification (OA). Previous studies have demonstrated that OA can have negative physiological consequences for calcifying organisms, especially during early life-history stages. However, much of the previous research has focused on static exposure to future OA conditions, rather than variable exposure to elevated pCO 2 , which is more ecologically relevant for nearshore species. This study examines the effects of OA on embryonic and larval Pacific razor clams (Siliqua patula), a bivalve that produces a concretion during early shell development. Larvae were spawned and cultured over 28 days under three pCO 2 treatments: a static high pCO 2 of 867 μatm, a variable, diel pCO 2 of 357 to 867 μatm, and an ambient pCO 2 of 357 μatm. Our results indicate that the calcium carbonate polymorphism of the concretion phase of S. patula was amorphous calcium carbonate which transitioned to vaterite during the advanced D-veliger stage, with a final polymorphic shift to aragonite in adults, suggesting an increased vulnerability to dissolution under OA. However, exposure to elevated pCO 2 appeared to accelerate the transition of larval S. patula from the concretion stage of shell development to complete calcification. There was no significant impact of OA exposure to elevated or variable pCO 2 conditions on S. patula growth or HSP70 and calmodulin gene expression. This is the first experimental study examining the response of a concretion producing bivalve to future predicted OA conditions and has important implications for experimentation on larval mollusks and bivalve management. Still Image Ocean acidification Frontiers: Figshare
institution Open Polar
collection Frontiers: Figshare
op_collection_id ftfrontimediafig
language unknown
topic Oceanography
Marine Biology
Marine Geoscience
Biological Oceanography
Chemical Oceanography
Physical Oceanography
Marine Engineering
ocean acidification
vaterite
biomineralogy
Siliqua patula
variability
larval response
amorphous calcium carbonate (ACC)
spellingShingle Oceanography
Marine Biology
Marine Geoscience
Biological Oceanography
Chemical Oceanography
Physical Oceanography
Marine Engineering
ocean acidification
vaterite
biomineralogy
Siliqua patula
variability
larval response
amorphous calcium carbonate (ACC)
Marina W. Alcantar
Jeff Hetrick
Jacqueline Ramsay
Amanda L. Kelley
Image_1_Examining the impacts of elevated, variable pCO2 on larval Pacific razor clams (Siliqua patula) in Alaska.pdf
topic_facet Oceanography
Marine Biology
Marine Geoscience
Biological Oceanography
Chemical Oceanography
Physical Oceanography
Marine Engineering
ocean acidification
vaterite
biomineralogy
Siliqua patula
variability
larval response
amorphous calcium carbonate (ACC)
description An increase in anthropogenic carbon dioxide is driving oceanic chemical shifts resulting in a long-term global decrease in ocean pH, colloquially termed ocean acidification (OA). Previous studies have demonstrated that OA can have negative physiological consequences for calcifying organisms, especially during early life-history stages. However, much of the previous research has focused on static exposure to future OA conditions, rather than variable exposure to elevated pCO 2 , which is more ecologically relevant for nearshore species. This study examines the effects of OA on embryonic and larval Pacific razor clams (Siliqua patula), a bivalve that produces a concretion during early shell development. Larvae were spawned and cultured over 28 days under three pCO 2 treatments: a static high pCO 2 of 867 μatm, a variable, diel pCO 2 of 357 to 867 μatm, and an ambient pCO 2 of 357 μatm. Our results indicate that the calcium carbonate polymorphism of the concretion phase of S. patula was amorphous calcium carbonate which transitioned to vaterite during the advanced D-veliger stage, with a final polymorphic shift to aragonite in adults, suggesting an increased vulnerability to dissolution under OA. However, exposure to elevated pCO 2 appeared to accelerate the transition of larval S. patula from the concretion stage of shell development to complete calcification. There was no significant impact of OA exposure to elevated or variable pCO 2 conditions on S. patula growth or HSP70 and calmodulin gene expression. This is the first experimental study examining the response of a concretion producing bivalve to future predicted OA conditions and has important implications for experimentation on larval mollusks and bivalve management.
format Still Image
author Marina W. Alcantar
Jeff Hetrick
Jacqueline Ramsay
Amanda L. Kelley
author_facet Marina W. Alcantar
Jeff Hetrick
Jacqueline Ramsay
Amanda L. Kelley
author_sort Marina W. Alcantar
title Image_1_Examining the impacts of elevated, variable pCO2 on larval Pacific razor clams (Siliqua patula) in Alaska.pdf
title_short Image_1_Examining the impacts of elevated, variable pCO2 on larval Pacific razor clams (Siliqua patula) in Alaska.pdf
title_full Image_1_Examining the impacts of elevated, variable pCO2 on larval Pacific razor clams (Siliqua patula) in Alaska.pdf
title_fullStr Image_1_Examining the impacts of elevated, variable pCO2 on larval Pacific razor clams (Siliqua patula) in Alaska.pdf
title_full_unstemmed Image_1_Examining the impacts of elevated, variable pCO2 on larval Pacific razor clams (Siliqua patula) in Alaska.pdf
title_sort image_1_examining the impacts of elevated, variable pco2 on larval pacific razor clams (siliqua patula) in alaska.pdf
publishDate 2024
url https://doi.org/10.3389/fmars.2024.1253702.s001
https://figshare.com/articles/figure/Image_1_Examining_the_impacts_of_elevated_variable_pCO2_on_larval_Pacific_razor_clams_Siliqua_patula_in_Alaska_pdf/25052108
genre Ocean acidification
genre_facet Ocean acidification
op_relation doi:10.3389/fmars.2024.1253702.s001
https://figshare.com/articles/figure/Image_1_Examining_the_impacts_of_elevated_variable_pCO2_on_larval_Pacific_razor_clams_Siliqua_patula_in_Alaska_pdf/25052108
op_rights CC BY 4.0
op_doi https://doi.org/10.3389/fmars.2024.1253702.s001
_version_ 1810469295213248512