DataSheet_1_The respiratory microbiota of three cohabiting beluga whales (Delphinapterus leucas) under human care.docx
We sampled the respiratory mucus from voluntary blowhole exhalations (“blow”) of three healthy beluga whales (Delphinapterus leucas) under professional human care. Blow samples were collected from three resident belugas, one adult male (M1) and two adult females (F1, F2), with voluntary behaviors vi...
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Online Access: | https://doi.org/10.3389/fmars.2023.1168623.s001 https://figshare.com/articles/dataset/DataSheet_1_The_respiratory_microbiota_of_three_cohabiting_beluga_whales_Delphinapterus_leucas_under_human_care_docx/24806037 |
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ftfrontimediafig:oai:figshare.com:article/24806037 2024-01-14T10:05:49+01:00 DataSheet_1_The respiratory microbiota of three cohabiting beluga whales (Delphinapterus leucas) under human care.docx Susan A. Smith Destiny V. Ropati Luciana F. Santoferrara Tracy A. Romano George B. McManus 2023-12-14T11:26:15Z https://doi.org/10.3389/fmars.2023.1168623.s001 https://figshare.com/articles/dataset/DataSheet_1_The_respiratory_microbiota_of_three_cohabiting_beluga_whales_Delphinapterus_leucas_under_human_care_docx/24806037 unknown doi:10.3389/fmars.2023.1168623.s001 https://figshare.com/articles/dataset/DataSheet_1_The_respiratory_microbiota_of_three_cohabiting_beluga_whales_Delphinapterus_leucas_under_human_care_docx/24806037 CC BY 4.0 Oceanography Marine Biology Marine Geoscience Biological Oceanography Chemical Oceanography Physical Oceanography Marine Engineering microbiome Delphinapterus leucas microbiota ciliate blowhole blowhole microbiota marine mammal Dataset 2023 ftfrontimediafig https://doi.org/10.3389/fmars.2023.1168623.s001 2023-12-21T00:15:42Z We sampled the respiratory mucus from voluntary blowhole exhalations (“blow”) of three healthy beluga whales (Delphinapterus leucas) under professional human care. Blow samples were collected from three resident belugas, one adult male (M1) and two adult females (F1, F2), with voluntary behaviors via non-invasive methods over three days in July 2021 (four days for M1). Samples were weighed and examined microscopically for the enumeration of eukaryotic and prokaryotic microbes, and then were used to evaluate carbon substrate use and taxonomic diversity of prokaryotic communities in the host respiratory sytem. Microscopical observations and 18S rRNA gene sequencing indicated the presence of eukaryotic microbiota, the ciliate genera Planilamina and Kyaroikeus in all three individuals. Exposure of samples to different metabolic carbon substrates indicated significant differences in the number of carbon sources usable by the prokaryotic communities of different whales (range: 11-25 sources), as well as a signficantly decreased diversity of carbon sources used by the community in the habitat water (5 sources). Sequencing of the hypervariable V4 region of the 16S rRNA gene revealed 19 amplicon sequence variants (ASVs) that were present in all whale samples. The oldest female D. leucas (F2) had the lowest overall diversity, and was significantly different from M1 and F1 in taxon composition, including an anomalously low ratio of Baccillota: Bacteroidota (0.01) compared to the other whales. In comparisons of microbial community composition, M1 had a significantly higher diversity than F1 and F2. These results suggest that attention should be given to regular microbiome sampling, and indicate a need for the pairing of microbiome and clinical data for animals in aquaria. Overall, these data contribute to the growing database on the core respiratory microbiota in cohabiting cetaceans under professional human care, indicate the utility of non-invasive sampling, and help characterize a baseline for healthy D. leucas. Dataset Beluga Beluga* Delphinapterus leucas Frontiers: Figshare |
institution |
Open Polar |
collection |
Frontiers: Figshare |
op_collection_id |
ftfrontimediafig |
language |
unknown |
topic |
Oceanography Marine Biology Marine Geoscience Biological Oceanography Chemical Oceanography Physical Oceanography Marine Engineering microbiome Delphinapterus leucas microbiota ciliate blowhole blowhole microbiota marine mammal |
spellingShingle |
Oceanography Marine Biology Marine Geoscience Biological Oceanography Chemical Oceanography Physical Oceanography Marine Engineering microbiome Delphinapterus leucas microbiota ciliate blowhole blowhole microbiota marine mammal Susan A. Smith Destiny V. Ropati Luciana F. Santoferrara Tracy A. Romano George B. McManus DataSheet_1_The respiratory microbiota of three cohabiting beluga whales (Delphinapterus leucas) under human care.docx |
topic_facet |
Oceanography Marine Biology Marine Geoscience Biological Oceanography Chemical Oceanography Physical Oceanography Marine Engineering microbiome Delphinapterus leucas microbiota ciliate blowhole blowhole microbiota marine mammal |
description |
We sampled the respiratory mucus from voluntary blowhole exhalations (“blow”) of three healthy beluga whales (Delphinapterus leucas) under professional human care. Blow samples were collected from three resident belugas, one adult male (M1) and two adult females (F1, F2), with voluntary behaviors via non-invasive methods over three days in July 2021 (four days for M1). Samples were weighed and examined microscopically for the enumeration of eukaryotic and prokaryotic microbes, and then were used to evaluate carbon substrate use and taxonomic diversity of prokaryotic communities in the host respiratory sytem. Microscopical observations and 18S rRNA gene sequencing indicated the presence of eukaryotic microbiota, the ciliate genera Planilamina and Kyaroikeus in all three individuals. Exposure of samples to different metabolic carbon substrates indicated significant differences in the number of carbon sources usable by the prokaryotic communities of different whales (range: 11-25 sources), as well as a signficantly decreased diversity of carbon sources used by the community in the habitat water (5 sources). Sequencing of the hypervariable V4 region of the 16S rRNA gene revealed 19 amplicon sequence variants (ASVs) that were present in all whale samples. The oldest female D. leucas (F2) had the lowest overall diversity, and was significantly different from M1 and F1 in taxon composition, including an anomalously low ratio of Baccillota: Bacteroidota (0.01) compared to the other whales. In comparisons of microbial community composition, M1 had a significantly higher diversity than F1 and F2. These results suggest that attention should be given to regular microbiome sampling, and indicate a need for the pairing of microbiome and clinical data for animals in aquaria. Overall, these data contribute to the growing database on the core respiratory microbiota in cohabiting cetaceans under professional human care, indicate the utility of non-invasive sampling, and help characterize a baseline for healthy D. leucas. |
format |
Dataset |
author |
Susan A. Smith Destiny V. Ropati Luciana F. Santoferrara Tracy A. Romano George B. McManus |
author_facet |
Susan A. Smith Destiny V. Ropati Luciana F. Santoferrara Tracy A. Romano George B. McManus |
author_sort |
Susan A. Smith |
title |
DataSheet_1_The respiratory microbiota of three cohabiting beluga whales (Delphinapterus leucas) under human care.docx |
title_short |
DataSheet_1_The respiratory microbiota of three cohabiting beluga whales (Delphinapterus leucas) under human care.docx |
title_full |
DataSheet_1_The respiratory microbiota of three cohabiting beluga whales (Delphinapterus leucas) under human care.docx |
title_fullStr |
DataSheet_1_The respiratory microbiota of three cohabiting beluga whales (Delphinapterus leucas) under human care.docx |
title_full_unstemmed |
DataSheet_1_The respiratory microbiota of three cohabiting beluga whales (Delphinapterus leucas) under human care.docx |
title_sort |
datasheet_1_the respiratory microbiota of three cohabiting beluga whales (delphinapterus leucas) under human care.docx |
publishDate |
2023 |
url |
https://doi.org/10.3389/fmars.2023.1168623.s001 https://figshare.com/articles/dataset/DataSheet_1_The_respiratory_microbiota_of_three_cohabiting_beluga_whales_Delphinapterus_leucas_under_human_care_docx/24806037 |
genre |
Beluga Beluga* Delphinapterus leucas |
genre_facet |
Beluga Beluga* Delphinapterus leucas |
op_relation |
doi:10.3389/fmars.2023.1168623.s001 https://figshare.com/articles/dataset/DataSheet_1_The_respiratory_microbiota_of_three_cohabiting_beluga_whales_Delphinapterus_leucas_under_human_care_docx/24806037 |
op_rights |
CC BY 4.0 |
op_doi |
https://doi.org/10.3389/fmars.2023.1168623.s001 |
_version_ |
1788060134062161920 |