Table_2_Mechanisms of carbon dioxide detection in the earthworm Dendrobaena veneta.xlsx
Introduction Carbon dioxide (CO 2 ) is a critical biological signal that is noxious to many animals at high concentrations. The earthworm Dendrobaena veneta lives in subterranean burrows containing high levels of CO 2 and respires through its skin. Despite the ecological and agricultural importance...
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ftfrontimediafig:oai:figshare.com:article/24655533 2024-09-15T18:01:42+00:00 Table_2_Mechanisms of carbon dioxide detection in the earthworm Dendrobaena veneta.xlsx Emily Jordan Smith Jennifer L. Ryan Sofia A. Lopresti Dastan B. S. Haghnazari Karleigh A. S. Anderson Sarah J. Lipson Erik C. Johnson Wayne L. Silver Cecil J. Saunders 2023-11-29T04:18:33Z https://doi.org/10.3389/fevo.2023.1202410.s002 https://figshare.com/articles/dataset/Table_2_Mechanisms_of_carbon_dioxide_detection_in_the_earthworm_Dendrobaena_veneta_xlsx/24655533 unknown doi:10.3389/fevo.2023.1202410.s002 https://figshare.com/articles/dataset/Table_2_Mechanisms_of_carbon_dioxide_detection_in_the_earthworm_Dendrobaena_veneta_xlsx/24655533 CC BY 4.0 Evolutionary Biology Ecology Invasive Species Ecology Landscape Ecology Conservation and Biodiversity Behavioural Ecology Community Ecology (excl. Invasive Species Ecology) Ecological Physiology Freshwater Ecology Marine and Estuarine Ecology (incl. Marine Ichthyology) Population Ecology Terrestrial Ecology Eisenia hortensis European nightcrawler carbonic anhydrases chemical senses chemosensory epithelial sodium channels (ENaCs) degenerin TRPA1 (transient receptor potential A1) Dataset 2023 ftfrontimediafig https://doi.org/10.3389/fevo.2023.1202410.s002 2024-08-19T06:19:53Z Introduction Carbon dioxide (CO 2 ) is a critical biological signal that is noxious to many animals at high concentrations. The earthworm Dendrobaena veneta lives in subterranean burrows containing high levels of CO 2 and respires through its skin. Despite the ecological and agricultural importance of earthworms, relatively little is known about how they make decisions in their environment, including their response to elevated levels of CO 2 . Methods To examine CO 2 detection in this species, we designed the exudate assay, in which we placed an earthworm in a sealed container, exposed it to varying concentrations of CO 2 for one minute, and recorded the amount of exudate secreted. Because earthworms excrete exudate in response to noxious stimuli, we hypothesized that the amount of exudate produced was proportional to the amount of irritation. We repeated these experiments after treatment with several blockers for molecules with potential involvement in CO 2 detection, including carbonic anhydrases, guanylate cyclase, TRPA1, ASICs, and OTOP channels. We also confirmed the presence of homologous transcripts for each of these gene families in an epithelial transcriptome for D. veneta. Additionally, since organisms often detect CO 2 levels indirectly by monitoring the conversion to carbonic acid (a weak acid), we used the exudate assay to evaluate aversion to additional weak acids (formic acid, acetic acid, and propionic acid). Results Earthworms excreted significantly more exudate in response to CO 2 in a dosage-dependent manner, and this response was muted by the general carbonic anhydrase inhibitor acetazolamide, the carbonic anhydrase IX/XII inhibitor indisulam, the calcium channel blocker ruthenium red, the sodium channel blocker amiloride, and the acid-sensing ion channel blocker diminazene aceturate. Discussion These data provide evidence of the role of carbonic anhydrase and epithelial sodium channels in earthworm CO 2 detection, establish that, similar to other subterranean-dwelling animals, earthworms are ... Dataset Carbonic acid Frontiers: Figshare |
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Frontiers: Figshare |
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ftfrontimediafig |
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unknown |
topic |
Evolutionary Biology Ecology Invasive Species Ecology Landscape Ecology Conservation and Biodiversity Behavioural Ecology Community Ecology (excl. Invasive Species Ecology) Ecological Physiology Freshwater Ecology Marine and Estuarine Ecology (incl. Marine Ichthyology) Population Ecology Terrestrial Ecology Eisenia hortensis European nightcrawler carbonic anhydrases chemical senses chemosensory epithelial sodium channels (ENaCs) degenerin TRPA1 (transient receptor potential A1) |
spellingShingle |
Evolutionary Biology Ecology Invasive Species Ecology Landscape Ecology Conservation and Biodiversity Behavioural Ecology Community Ecology (excl. Invasive Species Ecology) Ecological Physiology Freshwater Ecology Marine and Estuarine Ecology (incl. Marine Ichthyology) Population Ecology Terrestrial Ecology Eisenia hortensis European nightcrawler carbonic anhydrases chemical senses chemosensory epithelial sodium channels (ENaCs) degenerin TRPA1 (transient receptor potential A1) Emily Jordan Smith Jennifer L. Ryan Sofia A. Lopresti Dastan B. S. Haghnazari Karleigh A. S. Anderson Sarah J. Lipson Erik C. Johnson Wayne L. Silver Cecil J. Saunders Table_2_Mechanisms of carbon dioxide detection in the earthworm Dendrobaena veneta.xlsx |
topic_facet |
Evolutionary Biology Ecology Invasive Species Ecology Landscape Ecology Conservation and Biodiversity Behavioural Ecology Community Ecology (excl. Invasive Species Ecology) Ecological Physiology Freshwater Ecology Marine and Estuarine Ecology (incl. Marine Ichthyology) Population Ecology Terrestrial Ecology Eisenia hortensis European nightcrawler carbonic anhydrases chemical senses chemosensory epithelial sodium channels (ENaCs) degenerin TRPA1 (transient receptor potential A1) |
description |
Introduction Carbon dioxide (CO 2 ) is a critical biological signal that is noxious to many animals at high concentrations. The earthworm Dendrobaena veneta lives in subterranean burrows containing high levels of CO 2 and respires through its skin. Despite the ecological and agricultural importance of earthworms, relatively little is known about how they make decisions in their environment, including their response to elevated levels of CO 2 . Methods To examine CO 2 detection in this species, we designed the exudate assay, in which we placed an earthworm in a sealed container, exposed it to varying concentrations of CO 2 for one minute, and recorded the amount of exudate secreted. Because earthworms excrete exudate in response to noxious stimuli, we hypothesized that the amount of exudate produced was proportional to the amount of irritation. We repeated these experiments after treatment with several blockers for molecules with potential involvement in CO 2 detection, including carbonic anhydrases, guanylate cyclase, TRPA1, ASICs, and OTOP channels. We also confirmed the presence of homologous transcripts for each of these gene families in an epithelial transcriptome for D. veneta. Additionally, since organisms often detect CO 2 levels indirectly by monitoring the conversion to carbonic acid (a weak acid), we used the exudate assay to evaluate aversion to additional weak acids (formic acid, acetic acid, and propionic acid). Results Earthworms excreted significantly more exudate in response to CO 2 in a dosage-dependent manner, and this response was muted by the general carbonic anhydrase inhibitor acetazolamide, the carbonic anhydrase IX/XII inhibitor indisulam, the calcium channel blocker ruthenium red, the sodium channel blocker amiloride, and the acid-sensing ion channel blocker diminazene aceturate. Discussion These data provide evidence of the role of carbonic anhydrase and epithelial sodium channels in earthworm CO 2 detection, establish that, similar to other subterranean-dwelling animals, earthworms are ... |
format |
Dataset |
author |
Emily Jordan Smith Jennifer L. Ryan Sofia A. Lopresti Dastan B. S. Haghnazari Karleigh A. S. Anderson Sarah J. Lipson Erik C. Johnson Wayne L. Silver Cecil J. Saunders |
author_facet |
Emily Jordan Smith Jennifer L. Ryan Sofia A. Lopresti Dastan B. S. Haghnazari Karleigh A. S. Anderson Sarah J. Lipson Erik C. Johnson Wayne L. Silver Cecil J. Saunders |
author_sort |
Emily Jordan Smith |
title |
Table_2_Mechanisms of carbon dioxide detection in the earthworm Dendrobaena veneta.xlsx |
title_short |
Table_2_Mechanisms of carbon dioxide detection in the earthworm Dendrobaena veneta.xlsx |
title_full |
Table_2_Mechanisms of carbon dioxide detection in the earthworm Dendrobaena veneta.xlsx |
title_fullStr |
Table_2_Mechanisms of carbon dioxide detection in the earthworm Dendrobaena veneta.xlsx |
title_full_unstemmed |
Table_2_Mechanisms of carbon dioxide detection in the earthworm Dendrobaena veneta.xlsx |
title_sort |
table_2_mechanisms of carbon dioxide detection in the earthworm dendrobaena veneta.xlsx |
publishDate |
2023 |
url |
https://doi.org/10.3389/fevo.2023.1202410.s002 https://figshare.com/articles/dataset/Table_2_Mechanisms_of_carbon_dioxide_detection_in_the_earthworm_Dendrobaena_veneta_xlsx/24655533 |
genre |
Carbonic acid |
genre_facet |
Carbonic acid |
op_relation |
doi:10.3389/fevo.2023.1202410.s002 https://figshare.com/articles/dataset/Table_2_Mechanisms_of_carbon_dioxide_detection_in_the_earthworm_Dendrobaena_veneta_xlsx/24655533 |
op_rights |
CC BY 4.0 |
op_doi |
https://doi.org/10.3389/fevo.2023.1202410.s002 |
_version_ |
1810438791248216064 |