Image6_Transcriptomic response of Mytilus coruscus mantle to acute sea water acidification and shell damage.TIF
Mytilus coruscus is an economically important marine calcifier living in the Yangtze River estuary sea area, where seasonal fluctuations in natural pH occur owing to freshwater input, resulting in a rapid reduction in seawater pH. In addition, Mytilus constantly suffers from shell fracture or injury...
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ftfrontimediafig:oai:figshare.com:article/24440035 2023-11-12T04:24:02+01:00 Image6_Transcriptomic response of Mytilus coruscus mantle to acute sea water acidification and shell damage.TIF Zhi Liao Fei Liu Ying Wang Xiaojun Fan Yingao Li Jianyu He Isabella Buttino Xiaojun Yan Xiaolin Zhang Ge Shi 2023-10-26T04:24:43Z https://doi.org/10.3389/fphys.2023.1289655.s006 https://figshare.com/articles/figure/Image6_Transcriptomic_response_of_Mytilus_coruscus_mantle_to_acute_sea_water_acidification_and_shell_damage_TIF/24440035 unknown doi:10.3389/fphys.2023.1289655.s006 https://figshare.com/articles/figure/Image6_Transcriptomic_response_of_Mytilus_coruscus_mantle_to_acute_sea_water_acidification_and_shell_damage_TIF/24440035 CC BY 4.0 Physiology Exercise Physiology Nutritional Physiology Reproduction Cell Physiology Systems Physiology Animal Physiology - Biophysics Animal Physiology - Cell Animal Physiology - Systems Comparative Physiology Physiology not elsewhere classified Mytilus coruscus acute acidification shell damage biomineralization shell matrix protein Image Figure 2023 ftfrontimediafig https://doi.org/10.3389/fphys.2023.1289655.s006 2023-11-02T00:15:37Z Mytilus coruscus is an economically important marine calcifier living in the Yangtze River estuary sea area, where seasonal fluctuations in natural pH occur owing to freshwater input, resulting in a rapid reduction in seawater pH. In addition, Mytilus constantly suffers from shell fracture or injury in the natural environment, and the shell repair mechanisms in mussels have evolved to counteract shell injury. Therefore, we utilized shell-complete and shell-damaged Mytilus coruscus in this study and performed transcriptomic analysis of the mantle to investigate whether the expression of mantle-specific genes can be induced by acute seawater acidification and how the mantle responds to acute acidification during the shell repair process. We found that acute acidification induced more differentially expressed genes than shell damage in the mantle, and the biomineralization-related Gene Ontology terms and KEGG pathways were significantly enriched by these DEGs. Most DEGs were upregulated in enriched pathways, indicating the activation of biomineralization-related processes in the mussel mantle under acute acidification. The expression levels of some shell matrix proteins and antimicrobial peptides increased under acute acidification and/or shell damage, suggesting the molecular modulation of the mantle for the preparation and activation of the shell repairing and anti-infection under adverse environmental conditions. In addition, morphological and microstructural analyses were performed for the mantle edge and shell cross-section, and changes in the mantle secretory capacity and shell inner film system induced by the two stressors were observed. Our findings highlight the adaptation of M. coruscus in estuarine areas with dramatic fluctuations in pH and may prove instrumental in its ability to survive ocean acidification. Still Image Ocean acidification Frontiers: Figshare |
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Open Polar |
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Frontiers: Figshare |
op_collection_id |
ftfrontimediafig |
language |
unknown |
topic |
Physiology Exercise Physiology Nutritional Physiology Reproduction Cell Physiology Systems Physiology Animal Physiology - Biophysics Animal Physiology - Cell Animal Physiology - Systems Comparative Physiology Physiology not elsewhere classified Mytilus coruscus acute acidification shell damage biomineralization shell matrix protein |
spellingShingle |
Physiology Exercise Physiology Nutritional Physiology Reproduction Cell Physiology Systems Physiology Animal Physiology - Biophysics Animal Physiology - Cell Animal Physiology - Systems Comparative Physiology Physiology not elsewhere classified Mytilus coruscus acute acidification shell damage biomineralization shell matrix protein Zhi Liao Fei Liu Ying Wang Xiaojun Fan Yingao Li Jianyu He Isabella Buttino Xiaojun Yan Xiaolin Zhang Ge Shi Image6_Transcriptomic response of Mytilus coruscus mantle to acute sea water acidification and shell damage.TIF |
topic_facet |
Physiology Exercise Physiology Nutritional Physiology Reproduction Cell Physiology Systems Physiology Animal Physiology - Biophysics Animal Physiology - Cell Animal Physiology - Systems Comparative Physiology Physiology not elsewhere classified Mytilus coruscus acute acidification shell damage biomineralization shell matrix protein |
description |
Mytilus coruscus is an economically important marine calcifier living in the Yangtze River estuary sea area, where seasonal fluctuations in natural pH occur owing to freshwater input, resulting in a rapid reduction in seawater pH. In addition, Mytilus constantly suffers from shell fracture or injury in the natural environment, and the shell repair mechanisms in mussels have evolved to counteract shell injury. Therefore, we utilized shell-complete and shell-damaged Mytilus coruscus in this study and performed transcriptomic analysis of the mantle to investigate whether the expression of mantle-specific genes can be induced by acute seawater acidification and how the mantle responds to acute acidification during the shell repair process. We found that acute acidification induced more differentially expressed genes than shell damage in the mantle, and the biomineralization-related Gene Ontology terms and KEGG pathways were significantly enriched by these DEGs. Most DEGs were upregulated in enriched pathways, indicating the activation of biomineralization-related processes in the mussel mantle under acute acidification. The expression levels of some shell matrix proteins and antimicrobial peptides increased under acute acidification and/or shell damage, suggesting the molecular modulation of the mantle for the preparation and activation of the shell repairing and anti-infection under adverse environmental conditions. In addition, morphological and microstructural analyses were performed for the mantle edge and shell cross-section, and changes in the mantle secretory capacity and shell inner film system induced by the two stressors were observed. Our findings highlight the adaptation of M. coruscus in estuarine areas with dramatic fluctuations in pH and may prove instrumental in its ability to survive ocean acidification. |
format |
Still Image |
author |
Zhi Liao Fei Liu Ying Wang Xiaojun Fan Yingao Li Jianyu He Isabella Buttino Xiaojun Yan Xiaolin Zhang Ge Shi |
author_facet |
Zhi Liao Fei Liu Ying Wang Xiaojun Fan Yingao Li Jianyu He Isabella Buttino Xiaojun Yan Xiaolin Zhang Ge Shi |
author_sort |
Zhi Liao |
title |
Image6_Transcriptomic response of Mytilus coruscus mantle to acute sea water acidification and shell damage.TIF |
title_short |
Image6_Transcriptomic response of Mytilus coruscus mantle to acute sea water acidification and shell damage.TIF |
title_full |
Image6_Transcriptomic response of Mytilus coruscus mantle to acute sea water acidification and shell damage.TIF |
title_fullStr |
Image6_Transcriptomic response of Mytilus coruscus mantle to acute sea water acidification and shell damage.TIF |
title_full_unstemmed |
Image6_Transcriptomic response of Mytilus coruscus mantle to acute sea water acidification and shell damage.TIF |
title_sort |
image6_transcriptomic response of mytilus coruscus mantle to acute sea water acidification and shell damage.tif |
publishDate |
2023 |
url |
https://doi.org/10.3389/fphys.2023.1289655.s006 https://figshare.com/articles/figure/Image6_Transcriptomic_response_of_Mytilus_coruscus_mantle_to_acute_sea_water_acidification_and_shell_damage_TIF/24440035 |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_relation |
doi:10.3389/fphys.2023.1289655.s006 https://figshare.com/articles/figure/Image6_Transcriptomic_response_of_Mytilus_coruscus_mantle_to_acute_sea_water_acidification_and_shell_damage_TIF/24440035 |
op_rights |
CC BY 4.0 |
op_doi |
https://doi.org/10.3389/fphys.2023.1289655.s006 |
_version_ |
1782338624901087232 |