Data_Sheet_1_Mining of key genes for cold adaptation from Pseudomonas fragi D12 and analysis of its cold-adaptation mechanism.docx
The psychrotroph Pseudomonas fragi D12, which grew strongly under low temperatures, was screened from tundra soil collected from the permanent alpine zone on Changbai Mountain. To mine the genes critical for cold tolerance and to investigate the cold-adaptation mechanism, whole-genome sequencing, co...
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ftfrontimediafig:oai:figshare.com:article/23633094 2024-09-09T20:12:24+00:00 Data_Sheet_1_Mining of key genes for cold adaptation from Pseudomonas fragi D12 and analysis of its cold-adaptation mechanism.docx Changjie Bao Muzi Li Xuhui Zhao Jia Shi Yehui Liu Na Zhang Yuqi Zhou Jie Ma Guang Chen Sitong Zhang Huan Chen 2023-07-06T04:20:26Z https://doi.org/10.3389/fmicb.2023.1215837.s001 https://figshare.com/articles/dataset/Data_Sheet_1_Mining_of_key_genes_for_cold_adaptation_from_Pseudomonas_fragi_D12_and_analysis_of_its_cold-adaptation_mechanism_docx/23633094 unknown doi:10.3389/fmicb.2023.1215837.s001 https://figshare.com/articles/dataset/Data_Sheet_1_Mining_of_key_genes_for_cold_adaptation_from_Pseudomonas_fragi_D12_and_analysis_of_its_cold-adaptation_mechanism_docx/23633094 CC BY 4.0 Microbiology Microbial Genetics Microbial Ecology Mycology psychrotrophs cold-adaptation mechanism genomics transcriptomics Pseudomonas fragi Dataset 2023 ftfrontimediafig https://doi.org/10.3389/fmicb.2023.1215837.s001 2024-08-19T06:20:04Z The psychrotroph Pseudomonas fragi D12, which grew strongly under low temperatures, was screened from tundra soil collected from the permanent alpine zone on Changbai Mountain. To mine the genes critical for cold tolerance and to investigate the cold-adaptation mechanism, whole-genome sequencing, comparative genomic analysis, and transcriptome analysis were performed with P. fragi. A total of 124 potential cold adaptation genes were identified, including nineteen unique cold-adaptive genes were detected in the genome of P. fragi D12. Three unique genes associated with pili protein were significantly upregulated at different degrees of low temperature, which may be the key to the strong low-temperature adaptability of P. fragi D12. Meanwhile, we were pleasantly surprised to find that Pseudomonas fragi D12 exhibited different cold-adaptation mechanisms under different temperature changes. When the temperature declined from 30°C to 15°C, the response included maintenance of the fluidity of cell membranes, increased production of extracellular polymers, elevation in the content of compatibility solutes, and reduction in the content of reactive oxygen species, thereby providing a stable metabolic environment. When the temperature decreased from 15°C to 4°C, the response mainly included increases in the expression of molecular chaperones and transcription factors, enabling the bacteria to restore normal transcription and translation. The response mechanism of P. fragi D12 to low-temperature exposure is discussed. The results provide new ideas for the cold-adaptation mechanism of cold-tolerant microorganisms. Dataset Tundra Frontiers: Figshare |
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Open Polar |
collection |
Frontiers: Figshare |
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ftfrontimediafig |
language |
unknown |
topic |
Microbiology Microbial Genetics Microbial Ecology Mycology psychrotrophs cold-adaptation mechanism genomics transcriptomics Pseudomonas fragi |
spellingShingle |
Microbiology Microbial Genetics Microbial Ecology Mycology psychrotrophs cold-adaptation mechanism genomics transcriptomics Pseudomonas fragi Changjie Bao Muzi Li Xuhui Zhao Jia Shi Yehui Liu Na Zhang Yuqi Zhou Jie Ma Guang Chen Sitong Zhang Huan Chen Data_Sheet_1_Mining of key genes for cold adaptation from Pseudomonas fragi D12 and analysis of its cold-adaptation mechanism.docx |
topic_facet |
Microbiology Microbial Genetics Microbial Ecology Mycology psychrotrophs cold-adaptation mechanism genomics transcriptomics Pseudomonas fragi |
description |
The psychrotroph Pseudomonas fragi D12, which grew strongly under low temperatures, was screened from tundra soil collected from the permanent alpine zone on Changbai Mountain. To mine the genes critical for cold tolerance and to investigate the cold-adaptation mechanism, whole-genome sequencing, comparative genomic analysis, and transcriptome analysis were performed with P. fragi. A total of 124 potential cold adaptation genes were identified, including nineteen unique cold-adaptive genes were detected in the genome of P. fragi D12. Three unique genes associated with pili protein were significantly upregulated at different degrees of low temperature, which may be the key to the strong low-temperature adaptability of P. fragi D12. Meanwhile, we were pleasantly surprised to find that Pseudomonas fragi D12 exhibited different cold-adaptation mechanisms under different temperature changes. When the temperature declined from 30°C to 15°C, the response included maintenance of the fluidity of cell membranes, increased production of extracellular polymers, elevation in the content of compatibility solutes, and reduction in the content of reactive oxygen species, thereby providing a stable metabolic environment. When the temperature decreased from 15°C to 4°C, the response mainly included increases in the expression of molecular chaperones and transcription factors, enabling the bacteria to restore normal transcription and translation. The response mechanism of P. fragi D12 to low-temperature exposure is discussed. The results provide new ideas for the cold-adaptation mechanism of cold-tolerant microorganisms. |
format |
Dataset |
author |
Changjie Bao Muzi Li Xuhui Zhao Jia Shi Yehui Liu Na Zhang Yuqi Zhou Jie Ma Guang Chen Sitong Zhang Huan Chen |
author_facet |
Changjie Bao Muzi Li Xuhui Zhao Jia Shi Yehui Liu Na Zhang Yuqi Zhou Jie Ma Guang Chen Sitong Zhang Huan Chen |
author_sort |
Changjie Bao |
title |
Data_Sheet_1_Mining of key genes for cold adaptation from Pseudomonas fragi D12 and analysis of its cold-adaptation mechanism.docx |
title_short |
Data_Sheet_1_Mining of key genes for cold adaptation from Pseudomonas fragi D12 and analysis of its cold-adaptation mechanism.docx |
title_full |
Data_Sheet_1_Mining of key genes for cold adaptation from Pseudomonas fragi D12 and analysis of its cold-adaptation mechanism.docx |
title_fullStr |
Data_Sheet_1_Mining of key genes for cold adaptation from Pseudomonas fragi D12 and analysis of its cold-adaptation mechanism.docx |
title_full_unstemmed |
Data_Sheet_1_Mining of key genes for cold adaptation from Pseudomonas fragi D12 and analysis of its cold-adaptation mechanism.docx |
title_sort |
data_sheet_1_mining of key genes for cold adaptation from pseudomonas fragi d12 and analysis of its cold-adaptation mechanism.docx |
publishDate |
2023 |
url |
https://doi.org/10.3389/fmicb.2023.1215837.s001 https://figshare.com/articles/dataset/Data_Sheet_1_Mining_of_key_genes_for_cold_adaptation_from_Pseudomonas_fragi_D12_and_analysis_of_its_cold-adaptation_mechanism_docx/23633094 |
genre |
Tundra |
genre_facet |
Tundra |
op_relation |
doi:10.3389/fmicb.2023.1215837.s001 https://figshare.com/articles/dataset/Data_Sheet_1_Mining_of_key_genes_for_cold_adaptation_from_Pseudomonas_fragi_D12_and_analysis_of_its_cold-adaptation_mechanism_docx/23633094 |
op_rights |
CC BY 4.0 |
op_doi |
https://doi.org/10.3389/fmicb.2023.1215837.s001 |
_version_ |
1809946989987627008 |