Table_2_Malassezia pachydermatis from brown bear: A comprehensive analysis reveals novel genotypes and distribution of all detected variants in domestic and wild animals.XLSX
Malassezia pachydermatis (phylum Basidiomycota, class Malasseziomycetes) is a zoophilic opportunistic pathogen with recognized potential for invasive infections in humans. Although this pathogenic yeast is widespread in nature, it has been primarily studied in domestic animals, so available data on...
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Online Access: | https://doi.org/10.3389/fmicb.2023.1151107.s002 https://figshare.com/articles/dataset/Table_2_Malassezia_pachydermatis_from_brown_bear_A_comprehensive_analysis_reveals_novel_genotypes_and_distribution_of_all_detected_variants_in_domestic_and_wild_animals_XLSX/22959278 |
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ftfrontimediafig:oai:figshare.com:article/22959278 2024-09-15T18:40:11+00:00 Table_2_Malassezia pachydermatis from brown bear: A comprehensive analysis reveals novel genotypes and distribution of all detected variants in domestic and wild animals.XLSX Suzana Hađina Branka Bruvo Mađarić Snježana Kazazić Tina Paradžik Slaven Reljić Ljiljana Pinter Đuro Huber Dušica Vujaklija 2023-05-19T11:05:56Z https://doi.org/10.3389/fmicb.2023.1151107.s002 https://figshare.com/articles/dataset/Table_2_Malassezia_pachydermatis_from_brown_bear_A_comprehensive_analysis_reveals_novel_genotypes_and_distribution_of_all_detected_variants_in_domestic_and_wild_animals_XLSX/22959278 unknown doi:10.3389/fmicb.2023.1151107.s002 https://figshare.com/articles/dataset/Table_2_Malassezia_pachydermatis_from_brown_bear_A_comprehensive_analysis_reveals_novel_genotypes_and_distribution_of_all_detected_variants_in_domestic_and_wild_animals_XLSX/22959278 CC BY 4.0 Microbiology Microbial Genetics Microbial Ecology Mycology novel genotypes brown bear Ursus arctos MALDI-TOF mass spectra Malassezia pachydermatis multilocus genotyping Dataset 2023 ftfrontimediafig https://doi.org/10.3389/fmicb.2023.1151107.s002 2024-08-19T06:19:58Z Malassezia pachydermatis (phylum Basidiomycota, class Malasseziomycetes) is a zoophilic opportunistic pathogen with recognized potential for invasive infections in humans. Although this pathogenic yeast is widespread in nature, it has been primarily studied in domestic animals, so available data on its genotypes in the wild are limited. In this study, 80 yeast isolates recovered from 42 brown bears (Ursus arctos) were identified as M. pachydermatis by a culture-based approach. MALDI-TOF mass spectrometry (MS) was used to endorse conventional identification. The majority of samples exhibited a high score fluctuation, with 42.5% of isolates generating the best scores in the range confident only for genus identification. However, the use of young biomass significantly improved the identification of M. pachydermatis at the species confidence level (98.8%). Importantly, the same MALDI-TOF MS efficiency would be achieved regardless of colony age if the cut-off value was lowered to ≥1.7. Genotyping of LSU, ITS1, CHS2, and β-tubulin markers identified four distinct genotypes in M. pachydermatis isolates. The most prevalent among them was the genotype previously found in dogs, indicating its transmission potential and adaptation to distantly related hosts. The other three genotypes are described for the first time in this study. However, only one of the genotypes consisted of all four loci with bear-specific sequences, indicating the formation of a strain specifically adapted to brown bears. Finally, we evaluated the specificity of the spectral profiles of the detected genotypes. MALDI-TOF MS exhibited great potential to detect subtle differences between all M. pachydermatis isolates and revealed distinct spectral profiles of bear-specific genotypes. Dataset Ursus arctos Frontiers: Figshare |
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Open Polar |
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Frontiers: Figshare |
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ftfrontimediafig |
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unknown |
topic |
Microbiology Microbial Genetics Microbial Ecology Mycology novel genotypes brown bear Ursus arctos MALDI-TOF mass spectra Malassezia pachydermatis multilocus genotyping |
spellingShingle |
Microbiology Microbial Genetics Microbial Ecology Mycology novel genotypes brown bear Ursus arctos MALDI-TOF mass spectra Malassezia pachydermatis multilocus genotyping Suzana Hađina Branka Bruvo Mađarić Snježana Kazazić Tina Paradžik Slaven Reljić Ljiljana Pinter Đuro Huber Dušica Vujaklija Table_2_Malassezia pachydermatis from brown bear: A comprehensive analysis reveals novel genotypes and distribution of all detected variants in domestic and wild animals.XLSX |
topic_facet |
Microbiology Microbial Genetics Microbial Ecology Mycology novel genotypes brown bear Ursus arctos MALDI-TOF mass spectra Malassezia pachydermatis multilocus genotyping |
description |
Malassezia pachydermatis (phylum Basidiomycota, class Malasseziomycetes) is a zoophilic opportunistic pathogen with recognized potential for invasive infections in humans. Although this pathogenic yeast is widespread in nature, it has been primarily studied in domestic animals, so available data on its genotypes in the wild are limited. In this study, 80 yeast isolates recovered from 42 brown bears (Ursus arctos) were identified as M. pachydermatis by a culture-based approach. MALDI-TOF mass spectrometry (MS) was used to endorse conventional identification. The majority of samples exhibited a high score fluctuation, with 42.5% of isolates generating the best scores in the range confident only for genus identification. However, the use of young biomass significantly improved the identification of M. pachydermatis at the species confidence level (98.8%). Importantly, the same MALDI-TOF MS efficiency would be achieved regardless of colony age if the cut-off value was lowered to ≥1.7. Genotyping of LSU, ITS1, CHS2, and β-tubulin markers identified four distinct genotypes in M. pachydermatis isolates. The most prevalent among them was the genotype previously found in dogs, indicating its transmission potential and adaptation to distantly related hosts. The other three genotypes are described for the first time in this study. However, only one of the genotypes consisted of all four loci with bear-specific sequences, indicating the formation of a strain specifically adapted to brown bears. Finally, we evaluated the specificity of the spectral profiles of the detected genotypes. MALDI-TOF MS exhibited great potential to detect subtle differences between all M. pachydermatis isolates and revealed distinct spectral profiles of bear-specific genotypes. |
format |
Dataset |
author |
Suzana Hađina Branka Bruvo Mađarić Snježana Kazazić Tina Paradžik Slaven Reljić Ljiljana Pinter Đuro Huber Dušica Vujaklija |
author_facet |
Suzana Hađina Branka Bruvo Mađarić Snježana Kazazić Tina Paradžik Slaven Reljić Ljiljana Pinter Đuro Huber Dušica Vujaklija |
author_sort |
Suzana Hađina |
title |
Table_2_Malassezia pachydermatis from brown bear: A comprehensive analysis reveals novel genotypes and distribution of all detected variants in domestic and wild animals.XLSX |
title_short |
Table_2_Malassezia pachydermatis from brown bear: A comprehensive analysis reveals novel genotypes and distribution of all detected variants in domestic and wild animals.XLSX |
title_full |
Table_2_Malassezia pachydermatis from brown bear: A comprehensive analysis reveals novel genotypes and distribution of all detected variants in domestic and wild animals.XLSX |
title_fullStr |
Table_2_Malassezia pachydermatis from brown bear: A comprehensive analysis reveals novel genotypes and distribution of all detected variants in domestic and wild animals.XLSX |
title_full_unstemmed |
Table_2_Malassezia pachydermatis from brown bear: A comprehensive analysis reveals novel genotypes and distribution of all detected variants in domestic and wild animals.XLSX |
title_sort |
table_2_malassezia pachydermatis from brown bear: a comprehensive analysis reveals novel genotypes and distribution of all detected variants in domestic and wild animals.xlsx |
publishDate |
2023 |
url |
https://doi.org/10.3389/fmicb.2023.1151107.s002 https://figshare.com/articles/dataset/Table_2_Malassezia_pachydermatis_from_brown_bear_A_comprehensive_analysis_reveals_novel_genotypes_and_distribution_of_all_detected_variants_in_domestic_and_wild_animals_XLSX/22959278 |
genre |
Ursus arctos |
genre_facet |
Ursus arctos |
op_relation |
doi:10.3389/fmicb.2023.1151107.s002 https://figshare.com/articles/dataset/Table_2_Malassezia_pachydermatis_from_brown_bear_A_comprehensive_analysis_reveals_novel_genotypes_and_distribution_of_all_detected_variants_in_domestic_and_wild_animals_XLSX/22959278 |
op_rights |
CC BY 4.0 |
op_doi |
https://doi.org/10.3389/fmicb.2023.1151107.s002 |
_version_ |
1810484501679177728 |