Table_1_Inactivated ostreid herpesvirus-1 induces an innate immune response in the Pacific oyster, Crassostrea gigas, hemocytes.docx
Infectious diseases are a major constraint to the expansion of shellfish production worldwide. Pacific oyster mortality syndrome (POMS), a polymicrobial disease triggered by the Ostreid herpesvirus-1 (OsHV-1), has devastated the global Pacific oyster (Crassostrea gigas) aquaculture industry. Recent...
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ftfrontimediafig:oai:figshare.com:article/22715575 2023-06-11T04:11:07+02:00 Table_1_Inactivated ostreid herpesvirus-1 induces an innate immune response in the Pacific oyster, Crassostrea gigas, hemocytes.docx Lizenn Delisle Anne Rolton Julien Vignier 2023-04-28T04:05:08Z https://doi.org/10.3389/fimmu.2023.1161145.s001 https://figshare.com/articles/dataset/Table_1_Inactivated_ostreid_herpesvirus-1_induces_an_innate_immune_response_in_the_Pacific_oyster_Crassostrea_gigas_hemocytes_docx/22715575 unknown doi:10.3389/fimmu.2023.1161145.s001 https://figshare.com/articles/dataset/Table_1_Inactivated_ostreid_herpesvirus-1_induces_an_innate_immune_response_in_the_Pacific_oyster_Crassostrea_gigas_hemocytes_docx/22715575 CC BY 4.0 Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology immune priming Ostreid herpesvirus-1 pseudo-vaccination innate immune memory flow cytometry reactive oxygen species droplet digital PCR Dataset 2023 ftfrontimediafig https://doi.org/10.3389/fimmu.2023.1161145.s001 2023-05-03T23:12:43Z Infectious diseases are a major constraint to the expansion of shellfish production worldwide. Pacific oyster mortality syndrome (POMS), a polymicrobial disease triggered by the Ostreid herpesvirus-1 (OsHV-1), has devastated the global Pacific oyster (Crassostrea gigas) aquaculture industry. Recent ground-breaking research revealed that C. gigas possess an immune memory, capable of adaption, which improves the immune response upon a second exposure to a pathogen. This paradigm shift opens the door for developing ‘vaccines’ to improve shellfish survival during disease outbreaks. In the present study, we developed an in-vitro assay using hemocytes – the main effectors of the C. gigas immune system – collected from juvenile oysters susceptible to OsHV-1. The potency of multiple antigen preparations (e.g., chemically and physically inactivated OsHV-1, viral DNA, and protein extracts) to stimulate an immune response in hemocytes was evaluated using flow cytometry and droplet digital PCR to measure immune-related subcellular functions and gene expression, respectively. The immune response to the different antigens was benchmarked against that of hemocytes treated with Poly (I:C). We identified 10 antigen preparations capable of inducing immune stimulation in hemocytes (ROS production and positively expressed immune- related genes) after 1 h of exposure, without causing cytotoxicity. These findings are significant, as they evidence the potential for priming the innate immunity of oysters using viral antigens, which may enable cost-effective therapeutic treatment to mitigate OsHV-1/POMS. Further testing of these antigen preparations using an in-vivo infection model is essential to validate promising candidate pseudo-vaccines. Dataset Crassostrea gigas Pacific oyster Frontiers: Figshare Pacific |
institution |
Open Polar |
collection |
Frontiers: Figshare |
op_collection_id |
ftfrontimediafig |
language |
unknown |
topic |
Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology immune priming Ostreid herpesvirus-1 pseudo-vaccination innate immune memory flow cytometry reactive oxygen species droplet digital PCR |
spellingShingle |
Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology immune priming Ostreid herpesvirus-1 pseudo-vaccination innate immune memory flow cytometry reactive oxygen species droplet digital PCR Lizenn Delisle Anne Rolton Julien Vignier Table_1_Inactivated ostreid herpesvirus-1 induces an innate immune response in the Pacific oyster, Crassostrea gigas, hemocytes.docx |
topic_facet |
Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology immune priming Ostreid herpesvirus-1 pseudo-vaccination innate immune memory flow cytometry reactive oxygen species droplet digital PCR |
description |
Infectious diseases are a major constraint to the expansion of shellfish production worldwide. Pacific oyster mortality syndrome (POMS), a polymicrobial disease triggered by the Ostreid herpesvirus-1 (OsHV-1), has devastated the global Pacific oyster (Crassostrea gigas) aquaculture industry. Recent ground-breaking research revealed that C. gigas possess an immune memory, capable of adaption, which improves the immune response upon a second exposure to a pathogen. This paradigm shift opens the door for developing ‘vaccines’ to improve shellfish survival during disease outbreaks. In the present study, we developed an in-vitro assay using hemocytes – the main effectors of the C. gigas immune system – collected from juvenile oysters susceptible to OsHV-1. The potency of multiple antigen preparations (e.g., chemically and physically inactivated OsHV-1, viral DNA, and protein extracts) to stimulate an immune response in hemocytes was evaluated using flow cytometry and droplet digital PCR to measure immune-related subcellular functions and gene expression, respectively. The immune response to the different antigens was benchmarked against that of hemocytes treated with Poly (I:C). We identified 10 antigen preparations capable of inducing immune stimulation in hemocytes (ROS production and positively expressed immune- related genes) after 1 h of exposure, without causing cytotoxicity. These findings are significant, as they evidence the potential for priming the innate immunity of oysters using viral antigens, which may enable cost-effective therapeutic treatment to mitigate OsHV-1/POMS. Further testing of these antigen preparations using an in-vivo infection model is essential to validate promising candidate pseudo-vaccines. |
format |
Dataset |
author |
Lizenn Delisle Anne Rolton Julien Vignier |
author_facet |
Lizenn Delisle Anne Rolton Julien Vignier |
author_sort |
Lizenn Delisle |
title |
Table_1_Inactivated ostreid herpesvirus-1 induces an innate immune response in the Pacific oyster, Crassostrea gigas, hemocytes.docx |
title_short |
Table_1_Inactivated ostreid herpesvirus-1 induces an innate immune response in the Pacific oyster, Crassostrea gigas, hemocytes.docx |
title_full |
Table_1_Inactivated ostreid herpesvirus-1 induces an innate immune response in the Pacific oyster, Crassostrea gigas, hemocytes.docx |
title_fullStr |
Table_1_Inactivated ostreid herpesvirus-1 induces an innate immune response in the Pacific oyster, Crassostrea gigas, hemocytes.docx |
title_full_unstemmed |
Table_1_Inactivated ostreid herpesvirus-1 induces an innate immune response in the Pacific oyster, Crassostrea gigas, hemocytes.docx |
title_sort |
table_1_inactivated ostreid herpesvirus-1 induces an innate immune response in the pacific oyster, crassostrea gigas, hemocytes.docx |
publishDate |
2023 |
url |
https://doi.org/10.3389/fimmu.2023.1161145.s001 https://figshare.com/articles/dataset/Table_1_Inactivated_ostreid_herpesvirus-1_induces_an_innate_immune_response_in_the_Pacific_oyster_Crassostrea_gigas_hemocytes_docx/22715575 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_relation |
doi:10.3389/fimmu.2023.1161145.s001 https://figshare.com/articles/dataset/Table_1_Inactivated_ostreid_herpesvirus-1_induces_an_innate_immune_response_in_the_Pacific_oyster_Crassostrea_gigas_hemocytes_docx/22715575 |
op_rights |
CC BY 4.0 |
op_doi |
https://doi.org/10.3389/fimmu.2023.1161145.s001 |
_version_ |
1768385999828680704 |