Table_1_Actinobacteria from Arctic and Atlantic deep-sea sediments—Biodiversity and bioactive potential.pdf
The deep-sea covers over 70% of the Earth’s surface and harbors predominantly uncharacterized bacterial communities. Actinobacteria are the major prokaryotic source of bioactive natural products that find their way into drug discovery programs, and the deep-sea is a promising source of biotechnologi...
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ftfrontimediafig:oai:figshare.com:article/22362304 2024-09-09T19:22:41+00:00 Table_1_Actinobacteria from Arctic and Atlantic deep-sea sediments—Biodiversity and bioactive potential.pdf Inês Ribeiro Jorge T. Antunes Diogo A. M. Alexandrino Maria Paola Tomasino Eduarda Almeida Ana Hilário Ralph Urbatzka Pedro N. Leão Ana P. Mucha Maria F. Carvalho 2023-03-30T13:53:42Z https://doi.org/10.3389/fmicb.2023.1158441.s003 https://figshare.com/articles/dataset/Table_1_Actinobacteria_from_Arctic_and_Atlantic_deep-sea_sediments_Biodiversity_and_bioactive_potential_pdf/22362304 unknown doi:10.3389/fmicb.2023.1158441.s003 https://figshare.com/articles/dataset/Table_1_Actinobacteria_from_Arctic_and_Atlantic_deep-sea_sediments_Biodiversity_and_bioactive_potential_pdf/22362304 CC BY 4.0 Microbiology Microbial Genetics Microbial Ecology Mycology actinobacteria antimicrobial anti-cancer anti-inflammatory deep-sea sediments metabarcoding Dataset 2023 ftfrontimediafig https://doi.org/10.3389/fmicb.2023.1158441.s003 2024-08-19T06:19:55Z The deep-sea covers over 70% of the Earth’s surface and harbors predominantly uncharacterized bacterial communities. Actinobacteria are the major prokaryotic source of bioactive natural products that find their way into drug discovery programs, and the deep-sea is a promising source of biotechnologically relevant actinobacteria. Previous studies on actinobacteria in deep-sea sediments were either regionally restricted or did not combine a community characterization with the analysis of their bioactive potential. Here we characterized the actinobacterial communities of upper layers of deep-sea sediments from the Arctic and the Atlantic (Azores and Madeira) ocean basins, employing 16S rRNA metabarcoding, and studied the biosynthetic potential of cultivable actinobacteria retrieved from those samples. Metabarcoding analysis showed that the actinobacterial composition varied between the sampled regions, with higher abundance in the Arctic samples but higher diversity in the Atlantic ones. Twenty actinobacterial genera were detected using metabarcoding, as a culture-independent method, while culture-dependent methods only allowed the identification of nine genera. Isolation of actinobacteria resulted on the retrieval of 44 isolates, mainly associated with Brachybacterium, Microbacterium, and Brevibacterium genera. Some of these isolates were only identified on a specific sampled region. Chemical extracts of the actinobacterial isolates were subsequently screened for their antimicrobial, anticancer and anti-inflammatory activities. Extracts from two Streptomyces strains demonstrated activity against Candida albicans. Additionally, eight extracts (obtained from Brachybacterium, Brevibacterium, Microbacterium, Rhodococcus, and Streptomyces isolates) showed significant activity against at least one of the tested cancer cell lines (HepG2 and T-47D). Furthermore, 15 actinobacterial extracts showed anti-inflammatory potential in the RAW 264.4 cell model assay, with no concomitant cytotoxic response. Dereplication and ... Dataset Arctic Frontiers: Figshare Arctic |
institution |
Open Polar |
collection |
Frontiers: Figshare |
op_collection_id |
ftfrontimediafig |
language |
unknown |
topic |
Microbiology Microbial Genetics Microbial Ecology Mycology actinobacteria antimicrobial anti-cancer anti-inflammatory deep-sea sediments metabarcoding |
spellingShingle |
Microbiology Microbial Genetics Microbial Ecology Mycology actinobacteria antimicrobial anti-cancer anti-inflammatory deep-sea sediments metabarcoding Inês Ribeiro Jorge T. Antunes Diogo A. M. Alexandrino Maria Paola Tomasino Eduarda Almeida Ana Hilário Ralph Urbatzka Pedro N. Leão Ana P. Mucha Maria F. Carvalho Table_1_Actinobacteria from Arctic and Atlantic deep-sea sediments—Biodiversity and bioactive potential.pdf |
topic_facet |
Microbiology Microbial Genetics Microbial Ecology Mycology actinobacteria antimicrobial anti-cancer anti-inflammatory deep-sea sediments metabarcoding |
description |
The deep-sea covers over 70% of the Earth’s surface and harbors predominantly uncharacterized bacterial communities. Actinobacteria are the major prokaryotic source of bioactive natural products that find their way into drug discovery programs, and the deep-sea is a promising source of biotechnologically relevant actinobacteria. Previous studies on actinobacteria in deep-sea sediments were either regionally restricted or did not combine a community characterization with the analysis of their bioactive potential. Here we characterized the actinobacterial communities of upper layers of deep-sea sediments from the Arctic and the Atlantic (Azores and Madeira) ocean basins, employing 16S rRNA metabarcoding, and studied the biosynthetic potential of cultivable actinobacteria retrieved from those samples. Metabarcoding analysis showed that the actinobacterial composition varied between the sampled regions, with higher abundance in the Arctic samples but higher diversity in the Atlantic ones. Twenty actinobacterial genera were detected using metabarcoding, as a culture-independent method, while culture-dependent methods only allowed the identification of nine genera. Isolation of actinobacteria resulted on the retrieval of 44 isolates, mainly associated with Brachybacterium, Microbacterium, and Brevibacterium genera. Some of these isolates were only identified on a specific sampled region. Chemical extracts of the actinobacterial isolates were subsequently screened for their antimicrobial, anticancer and anti-inflammatory activities. Extracts from two Streptomyces strains demonstrated activity against Candida albicans. Additionally, eight extracts (obtained from Brachybacterium, Brevibacterium, Microbacterium, Rhodococcus, and Streptomyces isolates) showed significant activity against at least one of the tested cancer cell lines (HepG2 and T-47D). Furthermore, 15 actinobacterial extracts showed anti-inflammatory potential in the RAW 264.4 cell model assay, with no concomitant cytotoxic response. Dereplication and ... |
format |
Dataset |
author |
Inês Ribeiro Jorge T. Antunes Diogo A. M. Alexandrino Maria Paola Tomasino Eduarda Almeida Ana Hilário Ralph Urbatzka Pedro N. Leão Ana P. Mucha Maria F. Carvalho |
author_facet |
Inês Ribeiro Jorge T. Antunes Diogo A. M. Alexandrino Maria Paola Tomasino Eduarda Almeida Ana Hilário Ralph Urbatzka Pedro N. Leão Ana P. Mucha Maria F. Carvalho |
author_sort |
Inês Ribeiro |
title |
Table_1_Actinobacteria from Arctic and Atlantic deep-sea sediments—Biodiversity and bioactive potential.pdf |
title_short |
Table_1_Actinobacteria from Arctic and Atlantic deep-sea sediments—Biodiversity and bioactive potential.pdf |
title_full |
Table_1_Actinobacteria from Arctic and Atlantic deep-sea sediments—Biodiversity and bioactive potential.pdf |
title_fullStr |
Table_1_Actinobacteria from Arctic and Atlantic deep-sea sediments—Biodiversity and bioactive potential.pdf |
title_full_unstemmed |
Table_1_Actinobacteria from Arctic and Atlantic deep-sea sediments—Biodiversity and bioactive potential.pdf |
title_sort |
table_1_actinobacteria from arctic and atlantic deep-sea sediments—biodiversity and bioactive potential.pdf |
publishDate |
2023 |
url |
https://doi.org/10.3389/fmicb.2023.1158441.s003 https://figshare.com/articles/dataset/Table_1_Actinobacteria_from_Arctic_and_Atlantic_deep-sea_sediments_Biodiversity_and_bioactive_potential_pdf/22362304 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_relation |
doi:10.3389/fmicb.2023.1158441.s003 https://figshare.com/articles/dataset/Table_1_Actinobacteria_from_Arctic_and_Atlantic_deep-sea_sediments_Biodiversity_and_bioactive_potential_pdf/22362304 |
op_rights |
CC BY 4.0 |
op_doi |
https://doi.org/10.3389/fmicb.2023.1158441.s003 |
_version_ |
1809762970122584064 |