Table_2_Metagenomic analyses of a microbial assemblage in a subglacial lake beneath the Vatnajökull ice cap, Iceland.DOCX
Skaftárkatlar are two subglacial lakes located beneath the Vatnajökull ice cap in Iceland associated with geothermal and volcanic activity. Previous studies of these lakes with ribosomal gene (16S rDNA) tag sequencing revealed a limited diversity of bacteria adapted to cold, dark, and nutrient-poor...
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ftfrontimediafig:oai:figshare.com:article/22359277 2024-09-15T18:11:45+00:00 Table_2_Metagenomic analyses of a microbial assemblage in a subglacial lake beneath the Vatnajökull ice cap, Iceland.DOCX Pauline Vannier Gregory K. Farrant Alexandra Klonowski Eric Gaidos Thorsteinn Thorsteinsson Viggó þór Marteinsson 2023-03-30T04:42:44Z https://doi.org/10.3389/fmicb.2023.1122184.s003 https://figshare.com/articles/dataset/Table_2_Metagenomic_analyses_of_a_microbial_assemblage_in_a_subglacial_lake_beneath_the_Vatnaj_kull_ice_cap_Iceland_DOCX/22359277 unknown doi:10.3389/fmicb.2023.1122184.s003 https://figshare.com/articles/dataset/Table_2_Metagenomic_analyses_of_a_microbial_assemblage_in_a_subglacial_lake_beneath_the_Vatnaj_kull_ice_cap_Iceland_DOCX/22359277 CC BY 4.0 Microbiology Microbial Genetics Microbial Ecology Mycology subglacial lakes Iceland microbial assemblage metagenome metabolism Dataset 2023 ftfrontimediafig https://doi.org/10.3389/fmicb.2023.1122184.s003 2024-08-19T06:19:55Z Skaftárkatlar are two subglacial lakes located beneath the Vatnajökull ice cap in Iceland associated with geothermal and volcanic activity. Previous studies of these lakes with ribosomal gene (16S rDNA) tag sequencing revealed a limited diversity of bacteria adapted to cold, dark, and nutrient-poor waters. In this study, we present analyses of metagenomes from the lake which give new insights into its microbial ecology. Analyses of the 16S rDNA genes in the metagenomes confirmed the existence of a low-diversity core microbial assemblage in the lake and insights into the potential metabolisms of the dominant members. Seven taxonomic genera, Sulfuricurvum, Sulfurospirillum, Acetobacterium, Pelobacter/Geobacter, Saccharibacteria, Caldisericum, and an unclassified member of Prolixibacteraceae, comprised more than 98% of the rDNA reads in the library. Functional characterisation of the lake metagenomes revealed complete metabolic pathways for sulphur cycling, nitrogen metabolism, carbon fixation via the reverse Krebs cycle, and acetogenesis. These results show that chemolithoautotrophy constitutes the main metabolism in this subglacial ecosystem. This assemblage and its metabolisms are not reflected in enrichment cultures, demonstrating the importance of in situ investigations of this environment. Dataset Ice cap Iceland Vatnajökull Frontiers: Figshare |
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Frontiers: Figshare |
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ftfrontimediafig |
language |
unknown |
topic |
Microbiology Microbial Genetics Microbial Ecology Mycology subglacial lakes Iceland microbial assemblage metagenome metabolism |
spellingShingle |
Microbiology Microbial Genetics Microbial Ecology Mycology subglacial lakes Iceland microbial assemblage metagenome metabolism Pauline Vannier Gregory K. Farrant Alexandra Klonowski Eric Gaidos Thorsteinn Thorsteinsson Viggó þór Marteinsson Table_2_Metagenomic analyses of a microbial assemblage in a subglacial lake beneath the Vatnajökull ice cap, Iceland.DOCX |
topic_facet |
Microbiology Microbial Genetics Microbial Ecology Mycology subglacial lakes Iceland microbial assemblage metagenome metabolism |
description |
Skaftárkatlar are two subglacial lakes located beneath the Vatnajökull ice cap in Iceland associated with geothermal and volcanic activity. Previous studies of these lakes with ribosomal gene (16S rDNA) tag sequencing revealed a limited diversity of bacteria adapted to cold, dark, and nutrient-poor waters. In this study, we present analyses of metagenomes from the lake which give new insights into its microbial ecology. Analyses of the 16S rDNA genes in the metagenomes confirmed the existence of a low-diversity core microbial assemblage in the lake and insights into the potential metabolisms of the dominant members. Seven taxonomic genera, Sulfuricurvum, Sulfurospirillum, Acetobacterium, Pelobacter/Geobacter, Saccharibacteria, Caldisericum, and an unclassified member of Prolixibacteraceae, comprised more than 98% of the rDNA reads in the library. Functional characterisation of the lake metagenomes revealed complete metabolic pathways for sulphur cycling, nitrogen metabolism, carbon fixation via the reverse Krebs cycle, and acetogenesis. These results show that chemolithoautotrophy constitutes the main metabolism in this subglacial ecosystem. This assemblage and its metabolisms are not reflected in enrichment cultures, demonstrating the importance of in situ investigations of this environment. |
format |
Dataset |
author |
Pauline Vannier Gregory K. Farrant Alexandra Klonowski Eric Gaidos Thorsteinn Thorsteinsson Viggó þór Marteinsson |
author_facet |
Pauline Vannier Gregory K. Farrant Alexandra Klonowski Eric Gaidos Thorsteinn Thorsteinsson Viggó þór Marteinsson |
author_sort |
Pauline Vannier |
title |
Table_2_Metagenomic analyses of a microbial assemblage in a subglacial lake beneath the Vatnajökull ice cap, Iceland.DOCX |
title_short |
Table_2_Metagenomic analyses of a microbial assemblage in a subglacial lake beneath the Vatnajökull ice cap, Iceland.DOCX |
title_full |
Table_2_Metagenomic analyses of a microbial assemblage in a subglacial lake beneath the Vatnajökull ice cap, Iceland.DOCX |
title_fullStr |
Table_2_Metagenomic analyses of a microbial assemblage in a subglacial lake beneath the Vatnajökull ice cap, Iceland.DOCX |
title_full_unstemmed |
Table_2_Metagenomic analyses of a microbial assemblage in a subglacial lake beneath the Vatnajökull ice cap, Iceland.DOCX |
title_sort |
table_2_metagenomic analyses of a microbial assemblage in a subglacial lake beneath the vatnajökull ice cap, iceland.docx |
publishDate |
2023 |
url |
https://doi.org/10.3389/fmicb.2023.1122184.s003 https://figshare.com/articles/dataset/Table_2_Metagenomic_analyses_of_a_microbial_assemblage_in_a_subglacial_lake_beneath_the_Vatnaj_kull_ice_cap_Iceland_DOCX/22359277 |
genre |
Ice cap Iceland Vatnajökull |
genre_facet |
Ice cap Iceland Vatnajökull |
op_relation |
doi:10.3389/fmicb.2023.1122184.s003 https://figshare.com/articles/dataset/Table_2_Metagenomic_analyses_of_a_microbial_assemblage_in_a_subglacial_lake_beneath_the_Vatnaj_kull_ice_cap_Iceland_DOCX/22359277 |
op_rights |
CC BY 4.0 |
op_doi |
https://doi.org/10.3389/fmicb.2023.1122184.s003 |
_version_ |
1810449328276242432 |