Image_1_Metagenomic analyses of a microbial assemblage in a subglacial lake beneath the Vatnajökull ice cap, Iceland.TIF
Skaftárkatlar are two subglacial lakes located beneath the Vatnajökull ice cap in Iceland associated with geothermal and volcanic activity. Previous studies of these lakes with ribosomal gene (16S rDNA) tag sequencing revealed a limited diversity of bacteria adapted to cold, dark, and nutrient-poor...
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ftfrontimediafig:oai:figshare.com:article/22359265 2023-05-15T16:38:09+02:00 Image_1_Metagenomic analyses of a microbial assemblage in a subglacial lake beneath the Vatnajökull ice cap, Iceland.TIF Pauline Vannier Gregory K. Farrant Alexandra Klonowski Eric Gaidos Thorsteinn Thorsteinsson Viggó þór Marteinsson 2023-03-30T04:42:43Z https://doi.org/10.3389/fmicb.2023.1122184.s001 https://figshare.com/articles/figure/Image_1_Metagenomic_analyses_of_a_microbial_assemblage_in_a_subglacial_lake_beneath_the_Vatnaj_kull_ice_cap_Iceland_TIF/22359265 unknown doi:10.3389/fmicb.2023.1122184.s001 https://figshare.com/articles/figure/Image_1_Metagenomic_analyses_of_a_microbial_assemblage_in_a_subglacial_lake_beneath_the_Vatnaj_kull_ice_cap_Iceland_TIF/22359265 CC BY 4.0 Microbiology Microbial Genetics Microbial Ecology Mycology subglacial lakes Iceland microbial assemblage metagenome metabolism Image Figure 2023 ftfrontimediafig https://doi.org/10.3389/fmicb.2023.1122184.s001 2023-04-05T23:13:34Z Skaftárkatlar are two subglacial lakes located beneath the Vatnajökull ice cap in Iceland associated with geothermal and volcanic activity. Previous studies of these lakes with ribosomal gene (16S rDNA) tag sequencing revealed a limited diversity of bacteria adapted to cold, dark, and nutrient-poor waters. In this study, we present analyses of metagenomes from the lake which give new insights into its microbial ecology. Analyses of the 16S rDNA genes in the metagenomes confirmed the existence of a low-diversity core microbial assemblage in the lake and insights into the potential metabolisms of the dominant members. Seven taxonomic genera, Sulfuricurvum, Sulfurospirillum, Acetobacterium, Pelobacter/Geobacter, Saccharibacteria, Caldisericum, and an unclassified member of Prolixibacteraceae, comprised more than 98% of the rDNA reads in the library. Functional characterisation of the lake metagenomes revealed complete metabolic pathways for sulphur cycling, nitrogen metabolism, carbon fixation via the reverse Krebs cycle, and acetogenesis. These results show that chemolithoautotrophy constitutes the main metabolism in this subglacial ecosystem. This assemblage and its metabolisms are not reflected in enrichment cultures, demonstrating the importance of in situ investigations of this environment. Still Image Ice cap Iceland Vatnajökull Frontiers: Figshare Krebs ENVELOPE(-61.467,-61.467,-64.633,-64.633) Vatnajökull ENVELOPE(-16.823,-16.823,64.420,64.420) |
institution |
Open Polar |
collection |
Frontiers: Figshare |
op_collection_id |
ftfrontimediafig |
language |
unknown |
topic |
Microbiology Microbial Genetics Microbial Ecology Mycology subglacial lakes Iceland microbial assemblage metagenome metabolism |
spellingShingle |
Microbiology Microbial Genetics Microbial Ecology Mycology subglacial lakes Iceland microbial assemblage metagenome metabolism Pauline Vannier Gregory K. Farrant Alexandra Klonowski Eric Gaidos Thorsteinn Thorsteinsson Viggó þór Marteinsson Image_1_Metagenomic analyses of a microbial assemblage in a subglacial lake beneath the Vatnajökull ice cap, Iceland.TIF |
topic_facet |
Microbiology Microbial Genetics Microbial Ecology Mycology subglacial lakes Iceland microbial assemblage metagenome metabolism |
description |
Skaftárkatlar are two subglacial lakes located beneath the Vatnajökull ice cap in Iceland associated with geothermal and volcanic activity. Previous studies of these lakes with ribosomal gene (16S rDNA) tag sequencing revealed a limited diversity of bacteria adapted to cold, dark, and nutrient-poor waters. In this study, we present analyses of metagenomes from the lake which give new insights into its microbial ecology. Analyses of the 16S rDNA genes in the metagenomes confirmed the existence of a low-diversity core microbial assemblage in the lake and insights into the potential metabolisms of the dominant members. Seven taxonomic genera, Sulfuricurvum, Sulfurospirillum, Acetobacterium, Pelobacter/Geobacter, Saccharibacteria, Caldisericum, and an unclassified member of Prolixibacteraceae, comprised more than 98% of the rDNA reads in the library. Functional characterisation of the lake metagenomes revealed complete metabolic pathways for sulphur cycling, nitrogen metabolism, carbon fixation via the reverse Krebs cycle, and acetogenesis. These results show that chemolithoautotrophy constitutes the main metabolism in this subglacial ecosystem. This assemblage and its metabolisms are not reflected in enrichment cultures, demonstrating the importance of in situ investigations of this environment. |
format |
Still Image |
author |
Pauline Vannier Gregory K. Farrant Alexandra Klonowski Eric Gaidos Thorsteinn Thorsteinsson Viggó þór Marteinsson |
author_facet |
Pauline Vannier Gregory K. Farrant Alexandra Klonowski Eric Gaidos Thorsteinn Thorsteinsson Viggó þór Marteinsson |
author_sort |
Pauline Vannier |
title |
Image_1_Metagenomic analyses of a microbial assemblage in a subglacial lake beneath the Vatnajökull ice cap, Iceland.TIF |
title_short |
Image_1_Metagenomic analyses of a microbial assemblage in a subglacial lake beneath the Vatnajökull ice cap, Iceland.TIF |
title_full |
Image_1_Metagenomic analyses of a microbial assemblage in a subglacial lake beneath the Vatnajökull ice cap, Iceland.TIF |
title_fullStr |
Image_1_Metagenomic analyses of a microbial assemblage in a subglacial lake beneath the Vatnajökull ice cap, Iceland.TIF |
title_full_unstemmed |
Image_1_Metagenomic analyses of a microbial assemblage in a subglacial lake beneath the Vatnajökull ice cap, Iceland.TIF |
title_sort |
image_1_metagenomic analyses of a microbial assemblage in a subglacial lake beneath the vatnajökull ice cap, iceland.tif |
publishDate |
2023 |
url |
https://doi.org/10.3389/fmicb.2023.1122184.s001 https://figshare.com/articles/figure/Image_1_Metagenomic_analyses_of_a_microbial_assemblage_in_a_subglacial_lake_beneath_the_Vatnaj_kull_ice_cap_Iceland_TIF/22359265 |
long_lat |
ENVELOPE(-61.467,-61.467,-64.633,-64.633) ENVELOPE(-16.823,-16.823,64.420,64.420) |
geographic |
Krebs Vatnajökull |
geographic_facet |
Krebs Vatnajökull |
genre |
Ice cap Iceland Vatnajökull |
genre_facet |
Ice cap Iceland Vatnajökull |
op_relation |
doi:10.3389/fmicb.2023.1122184.s001 https://figshare.com/articles/figure/Image_1_Metagenomic_analyses_of_a_microbial_assemblage_in_a_subglacial_lake_beneath_the_Vatnaj_kull_ice_cap_Iceland_TIF/22359265 |
op_rights |
CC BY 4.0 |
op_doi |
https://doi.org/10.3389/fmicb.2023.1122184.s001 |
_version_ |
1766028449329184768 |