DataSheet1_Enabling growth-decoupled Komagataella phaffii recombinant protein production based on the methanol-free PDH promoter.pdf

• Main Authors: Núria Bernat-Camps, Katharina Ebner, Veronika Schusterbauer, Jasmin Elgin Fischer, Miguel Angel Nieto-Taype, Francisco Valero, Anton Glieder, Xavier Garcia-Ortega
• Format: Dataset
• Language: unknown
• Published: 2023
• Subjects: Biotechnology; Biological Engineering; Genetic Engineering; Biomarkers; Biomaterials; Biomechanical Engineering; Biomedical Engineering not elsewhere classified; Synthetic Biology; Agricultural Marine Biotechnology; Bioremediation; Bioprocessing; Bioproduction and Bioproducts; Industrial Biotechnology Diagnostics (incl. Biosensors); Industrial Microbiology (incl. Biofeedstocks); Industrial Molecular Engineering of Nucleic Acids and Proteins; Industrial Biotechnology not elsewhere classified; Medical Biotechnology Diagnostics (incl. Biosensors); Medical Molecular Engineering of Nucleic Acids and Proteins; Regenerative Medicine (incl. Stem Cells and Tissue Engineering); Medical Biotechnology not elsewhere classified; Komagataella phaffii; PDH; recombinant protein production; methanol-free expression system; growth-decoupled bioprocess; promoter regulation; Pichia pastoris; Antarc*; Antarctica
• Online Access: https://doi.org/10.3389/fbioe.2023.1130583.s001; https://figshare.com/articles/dataset/DataSheet1_Enabling_growth-decoupled_Komagataella_phaffii_recombinant_protein_production_based_on_the_methanol-free_PDH_promoter_pdf/22323022

The current transition towards the circular bioeconomy requires a rational development of biorefineries to sustainably fulfill the present demands. The use of Komagataella phaffii (Pichia pastoris) can meet this challenge, since it has the capability to use crude glycerol as a carbon-source, a by-product from the biodiesel industry, while producing high- and low-added value products. Recombinant protein production (RPP) using K. phaffii has often been driven either by the methanol induced AOX1 promoter (P AOX1 ) and/or the constitutive GAP promoter (P GAP ). In the last years, strong efforts have been focused on developing novel expression systems that expand the toolbox variety of K. phaffii to efficiently produce diverse proteins that requires different strategies. In this work, a study was conducted towards the development of methanol-free expression system based on a heat-shock gene promoter (P DH ) using glycerol as sole carbon source. Using this promoter, the recombinant expression is strongly induced in carbon-starving conditions. The classical P GAP was used as a benchmark, taking for both strains the lipase B from Candida antarctica (CalB) as model protein. Titer of CalB expressed under P DH outperformed P GAP controlled expression in shake-flask cultivations when using a slow-release continuous feeding technology, confirming that P DH is induced under pseudo-starving conditions. This increase was also confirmed in fed-batch cultivations. Several optimization rounds were carried out for P DH under different feeding and osmolarity conditions. In all of them the P DH controlled process outperformed the P GAP one in regard to CalB titer. The best P DH approach reached 3.6-fold more specific productivity than P GAP fed-batch at low μ. Compared to the optimum approach for P GAP -based process, the best P DH fed-batch strategy resulted in 2.3-fold higher titer, while the specific productivity was very similar. To summarize, P DH is an inducible promoter that exhibited a non-coupled growth regulation showing ...