DataSheet_2_From a cell model to a fish trial: Immunomodulatory effects of heat-killed Lactiplantibacillus plantarum as a functional ingredient in aquafeeds for salmonids.pdf
Paraprobiotics (dead/inactivated probiotics) are promising candidates in functional feeds to promote growth performance, modulate intestinal microbiota and enhance immune response of fish. During industrial production, fish are exposed to several stressful conditions such as handling, sub-optimal nu...
Main Authors: | , , , , |
---|---|
Format: | Dataset |
Language: | unknown |
Published: |
2023
|
Subjects: | |
Online Access: | https://doi.org/10.3389/fimmu.2023.1125702.s002 https://figshare.com/articles/dataset/DataSheet_2_From_a_cell_model_to_a_fish_trial_Immunomodulatory_effects_of_heat-killed_Lactiplantibacillus_plantarum_as_a_functional_ingredient_in_aquafeeds_for_salmonids_pdf/22261036 |
id |
ftfrontimediafig:oai:figshare.com:article/22261036 |
---|---|
record_format |
openpolar |
spelling |
ftfrontimediafig:oai:figshare.com:article/22261036 2023-05-15T15:32:17+02:00 DataSheet_2_From a cell model to a fish trial: Immunomodulatory effects of heat-killed Lactiplantibacillus plantarum as a functional ingredient in aquafeeds for salmonids.pdf Sérgio Domingos Cardoso Rocha Peng Lei Byron Morales-Lange Liv Torunn Mydland Margareth Øverland 2023-03-13T04:27:41Z https://doi.org/10.3389/fimmu.2023.1125702.s002 https://figshare.com/articles/dataset/DataSheet_2_From_a_cell_model_to_a_fish_trial_Immunomodulatory_effects_of_heat-killed_Lactiplantibacillus_plantarum_as_a_functional_ingredient_in_aquafeeds_for_salmonids_pdf/22261036 unknown doi:10.3389/fimmu.2023.1125702.s002 https://figshare.com/articles/dataset/DataSheet_2_From_a_cell_model_to_a_fish_trial_Immunomodulatory_effects_of_heat-killed_Lactiplantibacillus_plantarum_as_a_functional_ingredient_in_aquafeeds_for_salmonids_pdf/22261036 CC BY 4.0 Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology HK L-137 heat-killed lactobacillus plantarum L-137 paraprobiotics RTgutGC Atlantic salmon immune-related biomarkers microbiota aquaculture Dataset 2023 ftfrontimediafig https://doi.org/10.3389/fimmu.2023.1125702.s002 2023-03-16T00:11:46Z Paraprobiotics (dead/inactivated probiotics) are promising candidates in functional feeds to promote growth performance, modulate intestinal microbiota and enhance immune response of fish. During industrial production, fish are exposed to several stressful conditions such as handling, sub-optimal nutrition and diseases that can lead to reduced growth, increased mortalities and large economical losses. Such problems can be mitigated by use of functional feeds, leading to more-sustainable aquaculture and improved animal welfare. Lactiplantibacillus plantarum strain L-137 is a common bacterium found in fermented Southeast Asian dish made from fish and rice. The benefits of its heat-killed form (HK L-137) related to growth performance and immunomodulation have been studied in farmed fish such as Nile Tilapia (Oreochromis niloticus), striped catfish (Pangasianodon hypophthalmus) and bighead catfish (Clarias macrocephalus). To study if such benefits can also be observed in salmonids, we worked both at in vitro level using an intestinal epithelium cell line from rainbow trout (Oncorhynchus mykiss; RTgutGC) stimulated with HK L-137 (Feed LP20™) and at in vivo level with pre-smolt Atlantic salmon (Salmo salar) fed HK L-137 at different inclusion levels (20, 100 and 500 mg of Feed LP20™ kg -1 feed). In RTgutGC, the results showed that the barrier function of the cell monolayer was strengthened along with an increased production of IL-1β and a decreased production of Anxa1, indicating a modulation of the immune response. Interestingly, a similar trend was detected at the in vivo level in distal intestine from fish fed the highest inclusion level of HK L-137. Here, a lower production of Anxa1 was also detected (after a 61-day feeding period) in addition to an increase of total plasma IgM in the same group. Furthermore, the RNA-seq analysis showed that HK L-137 was able to modulate the gene expression of pathways related to molecular function, biological process and cellular component in distal intestine, without ... Dataset Atlantic salmon Salmo salar Frontiers: Figshare |
institution |
Open Polar |
collection |
Frontiers: Figshare |
op_collection_id |
ftfrontimediafig |
language |
unknown |
topic |
Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology HK L-137 heat-killed lactobacillus plantarum L-137 paraprobiotics RTgutGC Atlantic salmon immune-related biomarkers microbiota aquaculture |
spellingShingle |
Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology HK L-137 heat-killed lactobacillus plantarum L-137 paraprobiotics RTgutGC Atlantic salmon immune-related biomarkers microbiota aquaculture Sérgio Domingos Cardoso Rocha Peng Lei Byron Morales-Lange Liv Torunn Mydland Margareth Øverland DataSheet_2_From a cell model to a fish trial: Immunomodulatory effects of heat-killed Lactiplantibacillus plantarum as a functional ingredient in aquafeeds for salmonids.pdf |
topic_facet |
Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology HK L-137 heat-killed lactobacillus plantarum L-137 paraprobiotics RTgutGC Atlantic salmon immune-related biomarkers microbiota aquaculture |
description |
Paraprobiotics (dead/inactivated probiotics) are promising candidates in functional feeds to promote growth performance, modulate intestinal microbiota and enhance immune response of fish. During industrial production, fish are exposed to several stressful conditions such as handling, sub-optimal nutrition and diseases that can lead to reduced growth, increased mortalities and large economical losses. Such problems can be mitigated by use of functional feeds, leading to more-sustainable aquaculture and improved animal welfare. Lactiplantibacillus plantarum strain L-137 is a common bacterium found in fermented Southeast Asian dish made from fish and rice. The benefits of its heat-killed form (HK L-137) related to growth performance and immunomodulation have been studied in farmed fish such as Nile Tilapia (Oreochromis niloticus), striped catfish (Pangasianodon hypophthalmus) and bighead catfish (Clarias macrocephalus). To study if such benefits can also be observed in salmonids, we worked both at in vitro level using an intestinal epithelium cell line from rainbow trout (Oncorhynchus mykiss; RTgutGC) stimulated with HK L-137 (Feed LP20™) and at in vivo level with pre-smolt Atlantic salmon (Salmo salar) fed HK L-137 at different inclusion levels (20, 100 and 500 mg of Feed LP20™ kg -1 feed). In RTgutGC, the results showed that the barrier function of the cell monolayer was strengthened along with an increased production of IL-1β and a decreased production of Anxa1, indicating a modulation of the immune response. Interestingly, a similar trend was detected at the in vivo level in distal intestine from fish fed the highest inclusion level of HK L-137. Here, a lower production of Anxa1 was also detected (after a 61-day feeding period) in addition to an increase of total plasma IgM in the same group. Furthermore, the RNA-seq analysis showed that HK L-137 was able to modulate the gene expression of pathways related to molecular function, biological process and cellular component in distal intestine, without ... |
format |
Dataset |
author |
Sérgio Domingos Cardoso Rocha Peng Lei Byron Morales-Lange Liv Torunn Mydland Margareth Øverland |
author_facet |
Sérgio Domingos Cardoso Rocha Peng Lei Byron Morales-Lange Liv Torunn Mydland Margareth Øverland |
author_sort |
Sérgio Domingos Cardoso Rocha |
title |
DataSheet_2_From a cell model to a fish trial: Immunomodulatory effects of heat-killed Lactiplantibacillus plantarum as a functional ingredient in aquafeeds for salmonids.pdf |
title_short |
DataSheet_2_From a cell model to a fish trial: Immunomodulatory effects of heat-killed Lactiplantibacillus plantarum as a functional ingredient in aquafeeds for salmonids.pdf |
title_full |
DataSheet_2_From a cell model to a fish trial: Immunomodulatory effects of heat-killed Lactiplantibacillus plantarum as a functional ingredient in aquafeeds for salmonids.pdf |
title_fullStr |
DataSheet_2_From a cell model to a fish trial: Immunomodulatory effects of heat-killed Lactiplantibacillus plantarum as a functional ingredient in aquafeeds for salmonids.pdf |
title_full_unstemmed |
DataSheet_2_From a cell model to a fish trial: Immunomodulatory effects of heat-killed Lactiplantibacillus plantarum as a functional ingredient in aquafeeds for salmonids.pdf |
title_sort |
datasheet_2_from a cell model to a fish trial: immunomodulatory effects of heat-killed lactiplantibacillus plantarum as a functional ingredient in aquafeeds for salmonids.pdf |
publishDate |
2023 |
url |
https://doi.org/10.3389/fimmu.2023.1125702.s002 https://figshare.com/articles/dataset/DataSheet_2_From_a_cell_model_to_a_fish_trial_Immunomodulatory_effects_of_heat-killed_Lactiplantibacillus_plantarum_as_a_functional_ingredient_in_aquafeeds_for_salmonids_pdf/22261036 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
doi:10.3389/fimmu.2023.1125702.s002 https://figshare.com/articles/dataset/DataSheet_2_From_a_cell_model_to_a_fish_trial_Immunomodulatory_effects_of_heat-killed_Lactiplantibacillus_plantarum_as_a_functional_ingredient_in_aquafeeds_for_salmonids_pdf/22261036 |
op_rights |
CC BY 4.0 |
op_doi |
https://doi.org/10.3389/fimmu.2023.1125702.s002 |
_version_ |
1766362803740868608 |