Table4_Preliminary insights on the metabolomics of Trichinella zimbabwensis infection in Sprague Dawley rats using GCxGC-TOF-MS (untargeted approach).DOCX
Trichinella infections have been documented globally and have been detected in wild and/or domestic animals except Antarctica. There is paucity of information in the metabolic responses of hosts during Trichinella infections and biomarkers for infection that can be used in the diagnosis of the disea...
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ftfrontimediafig:oai:figshare.com:article/22117601 2024-09-15T17:43:19+00:00 Table4_Preliminary insights on the metabolomics of Trichinella zimbabwensis infection in Sprague Dawley rats using GCxGC-TOF-MS (untargeted approach).DOCX I. S. Ndlovu Ekuyikeno Silas S. I. Tshilwane M. Chaisi A. Vosloo S. Mukaratirwa 2023-02-17T14:02:16Z https://doi.org/10.3389/fmolb.2023.1128542.s002 https://figshare.com/articles/dataset/Table4_Preliminary_insights_on_the_metabolomics_of_Trichinella_zimbabwensis_infection_in_Sprague_Dawley_rats_using_GCxGC-TOF-MS_untargeted_approach_DOCX/22117601 unknown doi:10.3389/fmolb.2023.1128542.s002 https://figshare.com/articles/dataset/Table4_Preliminary_insights_on_the_metabolomics_of_Trichinella_zimbabwensis_infection_in_Sprague_Dawley_rats_using_GCxGC-TOF-MS_untargeted_approach_DOCX/22117601 CC BY 4.0 Biochemistry Molecular Biology Structural Biology Enzymes Protein Trafficking Proteomics and Intermolecular Interactions (excl. Medical Proteomics) Receptors and Membrane Biology Signal Transduction Structural Biology (incl. Macromolecular Modelling) Synthetic Biology gas chromatographic time of flight mass spectrometry (GCxGC-TOF/MS) trichinellosis metabolomics serum Trichinella zimbabwensis Dataset 2023 ftfrontimediafig https://doi.org/10.3389/fmolb.2023.1128542.s002 2024-08-19T06:19:54Z Trichinella infections have been documented globally and have been detected in wild and/or domestic animals except Antarctica. There is paucity of information in the metabolic responses of hosts during Trichinella infections and biomarkers for infection that can be used in the diagnosis of the disease. The current study aimed to apply a non-targeted metabolomic approach to identify Trichinella zimbabwensis biomarkers including metabolic response from sera of infected Sprague-Dawley rats. Fifty-four male Sprague-Dawley rats were randomly assigned into T. zimbabwensis infected group (n = 36) and the non-infected control (n = 18). Results from the study showed that the metabolic signature of T. zimbabwensis infection consists of enriched methyl histidine metabolism, disturbance of the liver urea cycle, impeded TCA cycle, and upregulation of gluconeogenesis metabolism. The observed disturbance in the metabolic pathways was attributed to the effects caused by the parasite during its migration to the muscles resulting in downregulation of amino acids intermediates in the Trichinella-infected animals, and therefore affecting energy production and degradation of biomolecules. It was concluded that T. zimbabwensis infection caused an upregulation of amino acids; pipecolic acid, histidine, and urea, and upregulation of glucose and meso-Erythritol. Moreover, T. zimbabwensis infection caused upregulation of the fatty acids, retinoic acid, and acetic acid. These findings highlight the potential of metabolomics as a novel approach for fundamental investigations of host-pathogen interactions as well as for disease progression and prognosis. Dataset Antarc* Antarctica Frontiers: Figshare |
institution |
Open Polar |
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Frontiers: Figshare |
op_collection_id |
ftfrontimediafig |
language |
unknown |
topic |
Biochemistry Molecular Biology Structural Biology Enzymes Protein Trafficking Proteomics and Intermolecular Interactions (excl. Medical Proteomics) Receptors and Membrane Biology Signal Transduction Structural Biology (incl. Macromolecular Modelling) Synthetic Biology gas chromatographic time of flight mass spectrometry (GCxGC-TOF/MS) trichinellosis metabolomics serum Trichinella zimbabwensis |
spellingShingle |
Biochemistry Molecular Biology Structural Biology Enzymes Protein Trafficking Proteomics and Intermolecular Interactions (excl. Medical Proteomics) Receptors and Membrane Biology Signal Transduction Structural Biology (incl. Macromolecular Modelling) Synthetic Biology gas chromatographic time of flight mass spectrometry (GCxGC-TOF/MS) trichinellosis metabolomics serum Trichinella zimbabwensis I. S. Ndlovu Ekuyikeno Silas S. I. Tshilwane M. Chaisi A. Vosloo S. Mukaratirwa Table4_Preliminary insights on the metabolomics of Trichinella zimbabwensis infection in Sprague Dawley rats using GCxGC-TOF-MS (untargeted approach).DOCX |
topic_facet |
Biochemistry Molecular Biology Structural Biology Enzymes Protein Trafficking Proteomics and Intermolecular Interactions (excl. Medical Proteomics) Receptors and Membrane Biology Signal Transduction Structural Biology (incl. Macromolecular Modelling) Synthetic Biology gas chromatographic time of flight mass spectrometry (GCxGC-TOF/MS) trichinellosis metabolomics serum Trichinella zimbabwensis |
description |
Trichinella infections have been documented globally and have been detected in wild and/or domestic animals except Antarctica. There is paucity of information in the metabolic responses of hosts during Trichinella infections and biomarkers for infection that can be used in the diagnosis of the disease. The current study aimed to apply a non-targeted metabolomic approach to identify Trichinella zimbabwensis biomarkers including metabolic response from sera of infected Sprague-Dawley rats. Fifty-four male Sprague-Dawley rats were randomly assigned into T. zimbabwensis infected group (n = 36) and the non-infected control (n = 18). Results from the study showed that the metabolic signature of T. zimbabwensis infection consists of enriched methyl histidine metabolism, disturbance of the liver urea cycle, impeded TCA cycle, and upregulation of gluconeogenesis metabolism. The observed disturbance in the metabolic pathways was attributed to the effects caused by the parasite during its migration to the muscles resulting in downregulation of amino acids intermediates in the Trichinella-infected animals, and therefore affecting energy production and degradation of biomolecules. It was concluded that T. zimbabwensis infection caused an upregulation of amino acids; pipecolic acid, histidine, and urea, and upregulation of glucose and meso-Erythritol. Moreover, T. zimbabwensis infection caused upregulation of the fatty acids, retinoic acid, and acetic acid. These findings highlight the potential of metabolomics as a novel approach for fundamental investigations of host-pathogen interactions as well as for disease progression and prognosis. |
format |
Dataset |
author |
I. S. Ndlovu Ekuyikeno Silas S. I. Tshilwane M. Chaisi A. Vosloo S. Mukaratirwa |
author_facet |
I. S. Ndlovu Ekuyikeno Silas S. I. Tshilwane M. Chaisi A. Vosloo S. Mukaratirwa |
author_sort |
I. S. Ndlovu |
title |
Table4_Preliminary insights on the metabolomics of Trichinella zimbabwensis infection in Sprague Dawley rats using GCxGC-TOF-MS (untargeted approach).DOCX |
title_short |
Table4_Preliminary insights on the metabolomics of Trichinella zimbabwensis infection in Sprague Dawley rats using GCxGC-TOF-MS (untargeted approach).DOCX |
title_full |
Table4_Preliminary insights on the metabolomics of Trichinella zimbabwensis infection in Sprague Dawley rats using GCxGC-TOF-MS (untargeted approach).DOCX |
title_fullStr |
Table4_Preliminary insights on the metabolomics of Trichinella zimbabwensis infection in Sprague Dawley rats using GCxGC-TOF-MS (untargeted approach).DOCX |
title_full_unstemmed |
Table4_Preliminary insights on the metabolomics of Trichinella zimbabwensis infection in Sprague Dawley rats using GCxGC-TOF-MS (untargeted approach).DOCX |
title_sort |
table4_preliminary insights on the metabolomics of trichinella zimbabwensis infection in sprague dawley rats using gcxgc-tof-ms (untargeted approach).docx |
publishDate |
2023 |
url |
https://doi.org/10.3389/fmolb.2023.1128542.s002 https://figshare.com/articles/dataset/Table4_Preliminary_insights_on_the_metabolomics_of_Trichinella_zimbabwensis_infection_in_Sprague_Dawley_rats_using_GCxGC-TOF-MS_untargeted_approach_DOCX/22117601 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_relation |
doi:10.3389/fmolb.2023.1128542.s002 https://figshare.com/articles/dataset/Table4_Preliminary_insights_on_the_metabolomics_of_Trichinella_zimbabwensis_infection_in_Sprague_Dawley_rats_using_GCxGC-TOF-MS_untargeted_approach_DOCX/22117601 |
op_rights |
CC BY 4.0 |
op_doi |
https://doi.org/10.3389/fmolb.2023.1128542.s002 |
_version_ |
1810490242807889920 |