Data_Sheet_1_Spatial and temporal conversion of nitrogen using Arthrobacter sp. 24S4–2, a strain obtained from Antarctica.docx
According to average nucleotide identity (ANI) analysis of the complete genomes, strain 24S4–2 isolated from Antarctica is considered as a potential novel Arthrobacter species. Arthrobacter sp. 24S4–2 could grow and produce ammonium in nitrate or nitrite or even nitrogen free medium. Strain 24S4–2 w...
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ftfrontimediafig:oai:figshare.com:article/22099265 2024-09-15T17:46:14+00:00 Data_Sheet_1_Spatial and temporal conversion of nitrogen using Arthrobacter sp. 24S4–2, a strain obtained from Antarctica.docx Yixuan Liu Yumin Zhang Yudi Huang Jingjing Niu Jun Huang Xiaoya Peng Fang Peng 2023-02-15T05:29:11Z https://doi.org/10.3389/fmicb.2023.1040201.s001 https://figshare.com/articles/dataset/Data_Sheet_1_Spatial_and_temporal_conversion_of_nitrogen_using_Arthrobacter_sp_24S4_2_a_strain_obtained_from_Antarctica_docx/22099265 unknown doi:10.3389/fmicb.2023.1040201.s001 https://figshare.com/articles/dataset/Data_Sheet_1_Spatial_and_temporal_conversion_of_nitrogen_using_Arthrobacter_sp_24S4_2_a_strain_obtained_from_Antarctica_docx/22099265 CC BY 4.0 Microbiology Microbial Genetics Microbial Ecology Mycology Arthrobacter aerobic DNRA nitrogen storage vesicle structure spatial and temporal transformation of nitrogen Antarctica Dataset 2023 ftfrontimediafig https://doi.org/10.3389/fmicb.2023.1040201.s001 2024-08-19T06:19:54Z According to average nucleotide identity (ANI) analysis of the complete genomes, strain 24S4–2 isolated from Antarctica is considered as a potential novel Arthrobacter species. Arthrobacter sp. 24S4–2 could grow and produce ammonium in nitrate or nitrite or even nitrogen free medium. Strain 24S4–2 was discovered to accumulate nitrate/nitrite and subsequently convert nitrate to nitrite intracellularly when incubated in a nitrate/nitrite medium. In nitrogen-free medium, strain 24S4–2 not only reduced the accumulated nitrite for growth, but also secreted ammonia to the extracellular under aerobic condition, which was thought to be linked to nitrite reductase genes nirB, nirD, and nasA by the transcriptome and RT-qPCR analysis. A membrane-like vesicle structure was detected in the cell of strain 24S4–2 by transmission electron microscopy, which was thought to be the site of intracellular nitrogen supply accumulation and conversion. This spatial and temporal conversion process of nitrogen source helps the strain maintain development in the absence of nitrogen supply or a harsh environment, which is part of its adaption strategy to the Antarctic environment. This process may also play an important ecological role, that other bacteria in the environment would benefit from its extracellular nitrogen source secretion and nitrite consumption characteristics. Dataset Antarc* Antarctic Antarctica Frontiers: Figshare |
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Open Polar |
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Frontiers: Figshare |
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ftfrontimediafig |
language |
unknown |
topic |
Microbiology Microbial Genetics Microbial Ecology Mycology Arthrobacter aerobic DNRA nitrogen storage vesicle structure spatial and temporal transformation of nitrogen Antarctica |
spellingShingle |
Microbiology Microbial Genetics Microbial Ecology Mycology Arthrobacter aerobic DNRA nitrogen storage vesicle structure spatial and temporal transformation of nitrogen Antarctica Yixuan Liu Yumin Zhang Yudi Huang Jingjing Niu Jun Huang Xiaoya Peng Fang Peng Data_Sheet_1_Spatial and temporal conversion of nitrogen using Arthrobacter sp. 24S4–2, a strain obtained from Antarctica.docx |
topic_facet |
Microbiology Microbial Genetics Microbial Ecology Mycology Arthrobacter aerobic DNRA nitrogen storage vesicle structure spatial and temporal transformation of nitrogen Antarctica |
description |
According to average nucleotide identity (ANI) analysis of the complete genomes, strain 24S4–2 isolated from Antarctica is considered as a potential novel Arthrobacter species. Arthrobacter sp. 24S4–2 could grow and produce ammonium in nitrate or nitrite or even nitrogen free medium. Strain 24S4–2 was discovered to accumulate nitrate/nitrite and subsequently convert nitrate to nitrite intracellularly when incubated in a nitrate/nitrite medium. In nitrogen-free medium, strain 24S4–2 not only reduced the accumulated nitrite for growth, but also secreted ammonia to the extracellular under aerobic condition, which was thought to be linked to nitrite reductase genes nirB, nirD, and nasA by the transcriptome and RT-qPCR analysis. A membrane-like vesicle structure was detected in the cell of strain 24S4–2 by transmission electron microscopy, which was thought to be the site of intracellular nitrogen supply accumulation and conversion. This spatial and temporal conversion process of nitrogen source helps the strain maintain development in the absence of nitrogen supply or a harsh environment, which is part of its adaption strategy to the Antarctic environment. This process may also play an important ecological role, that other bacteria in the environment would benefit from its extracellular nitrogen source secretion and nitrite consumption characteristics. |
format |
Dataset |
author |
Yixuan Liu Yumin Zhang Yudi Huang Jingjing Niu Jun Huang Xiaoya Peng Fang Peng |
author_facet |
Yixuan Liu Yumin Zhang Yudi Huang Jingjing Niu Jun Huang Xiaoya Peng Fang Peng |
author_sort |
Yixuan Liu |
title |
Data_Sheet_1_Spatial and temporal conversion of nitrogen using Arthrobacter sp. 24S4–2, a strain obtained from Antarctica.docx |
title_short |
Data_Sheet_1_Spatial and temporal conversion of nitrogen using Arthrobacter sp. 24S4–2, a strain obtained from Antarctica.docx |
title_full |
Data_Sheet_1_Spatial and temporal conversion of nitrogen using Arthrobacter sp. 24S4–2, a strain obtained from Antarctica.docx |
title_fullStr |
Data_Sheet_1_Spatial and temporal conversion of nitrogen using Arthrobacter sp. 24S4–2, a strain obtained from Antarctica.docx |
title_full_unstemmed |
Data_Sheet_1_Spatial and temporal conversion of nitrogen using Arthrobacter sp. 24S4–2, a strain obtained from Antarctica.docx |
title_sort |
data_sheet_1_spatial and temporal conversion of nitrogen using arthrobacter sp. 24s4–2, a strain obtained from antarctica.docx |
publishDate |
2023 |
url |
https://doi.org/10.3389/fmicb.2023.1040201.s001 https://figshare.com/articles/dataset/Data_Sheet_1_Spatial_and_temporal_conversion_of_nitrogen_using_Arthrobacter_sp_24S4_2_a_strain_obtained_from_Antarctica_docx/22099265 |
genre |
Antarc* Antarctic Antarctica |
genre_facet |
Antarc* Antarctic Antarctica |
op_relation |
doi:10.3389/fmicb.2023.1040201.s001 https://figshare.com/articles/dataset/Data_Sheet_1_Spatial_and_temporal_conversion_of_nitrogen_using_Arthrobacter_sp_24S4_2_a_strain_obtained_from_Antarctica_docx/22099265 |
op_rights |
CC BY 4.0 |
op_doi |
https://doi.org/10.3389/fmicb.2023.1040201.s001 |
_version_ |
1810494238337531904 |