Table_1_Immunity of turbot Induced by inactivated vaccine of Aeromonas salmonicida from the perspective of DNA methylation.docx
Introduction DNA methylation was one of the most important modification in epigenetics and played an important role in immune response. Since the introduction of Scophthalmus maximus, the scale of breeding has continued to expand, during which diseases caused by various bacteria, viruses and parasit...
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ftfrontimediafig:oai:figshare.com:article/22046741 2023-05-15T18:15:47+02:00 Table_1_Immunity of turbot Induced by inactivated vaccine of Aeromonas salmonicida from the perspective of DNA methylation.docx Yingrui Li Lin Su Xiaofei Liu Huimin Guo Shun Zhou Yunji Xiu 2023-02-08T07:33:05Z https://doi.org/10.3389/fimmu.2023.1124322.s001 https://figshare.com/articles/dataset/Table_1_Immunity_of_turbot_Induced_by_inactivated_vaccine_of_Aeromonas_salmonicida_from_the_perspective_of_DNA_methylation_docx/22046741 unknown doi:10.3389/fimmu.2023.1124322.s001 https://figshare.com/articles/dataset/Table_1_Immunity_of_turbot_Induced_by_inactivated_vaccine_of_Aeromonas_salmonicida_from_the_perspective_of_DNA_methylation_docx/22046741 CC BY 4.0 CC-BY Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology DNA methylation Scophthalmus maximus Aeromonas salmonicida inactivated vaccine Whole Genome Bisulfite Sequencing transcriptome sequencing Dataset 2023 ftfrontimediafig https://doi.org/10.3389/fimmu.2023.1124322.s001 2023-02-09T00:08:37Z Introduction DNA methylation was one of the most important modification in epigenetics and played an important role in immune response. Since the introduction of Scophthalmus maximus, the scale of breeding has continued to expand, during which diseases caused by various bacteria, viruses and parasites have become increasingly serious. Therefore, the inactivated vaccines have been widely researched and used in the field of aquatic products with its unique advantages. However, the immune mechanism that occurred in turbot after immunization with inactivated vaccine of Aeromonas salmonicida was not clear. Methods In this study, differentially methylated regions (DMRs) were screened by Whole Genome Bisulfite Sequencing (WGBS) and significantly differentially expressed genes (DEGs) were screened by Transcriptome sequencing. Double luciferase report assay and DNA pull-down assay were further verified the DNA methylation state of the gene promoter region affected genes transcriptional activity after immunization with inactivated vaccine of Aeromonas salmonicida. Results A total of 8149 differentially methylated regions (DMRs) were screened, in which there were many immune-related genes with altered DNA methylation status. Meanwhile, 386 significantly differentially expressed genes (DEGs) were identified, many of which were significantly enriched in Toll-like receptor signaling pathway, NOD-like receptor signaling pathway and C-type lectin receptor signaling pathway. Combined analysis of WGBS results and RNA-seq results, a total of 9 DMRs of negatively regulated genes are located in the promoter region, including 2 hypermethylated genes with lower expression and 7 hypomethylated genes with higher expression. Then, two immune-related genes C5a anaphylatoxin chemotactic receptor 1-like (C5ar1-Like) and Eosinophil peroxidase-like (EPX-Like), were screened to explore the regulation mechanism of DNA methylation modification on their expression level. Moreover, the DNA methylation state of the gene promoter region affected ... Dataset Scophthalmus maximus Turbot Frontiers: Figshare |
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Open Polar |
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Frontiers: Figshare |
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ftfrontimediafig |
language |
unknown |
topic |
Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology DNA methylation Scophthalmus maximus Aeromonas salmonicida inactivated vaccine Whole Genome Bisulfite Sequencing transcriptome sequencing |
spellingShingle |
Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology DNA methylation Scophthalmus maximus Aeromonas salmonicida inactivated vaccine Whole Genome Bisulfite Sequencing transcriptome sequencing Yingrui Li Lin Su Xiaofei Liu Huimin Guo Shun Zhou Yunji Xiu Table_1_Immunity of turbot Induced by inactivated vaccine of Aeromonas salmonicida from the perspective of DNA methylation.docx |
topic_facet |
Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology DNA methylation Scophthalmus maximus Aeromonas salmonicida inactivated vaccine Whole Genome Bisulfite Sequencing transcriptome sequencing |
description |
Introduction DNA methylation was one of the most important modification in epigenetics and played an important role in immune response. Since the introduction of Scophthalmus maximus, the scale of breeding has continued to expand, during which diseases caused by various bacteria, viruses and parasites have become increasingly serious. Therefore, the inactivated vaccines have been widely researched and used in the field of aquatic products with its unique advantages. However, the immune mechanism that occurred in turbot after immunization with inactivated vaccine of Aeromonas salmonicida was not clear. Methods In this study, differentially methylated regions (DMRs) were screened by Whole Genome Bisulfite Sequencing (WGBS) and significantly differentially expressed genes (DEGs) were screened by Transcriptome sequencing. Double luciferase report assay and DNA pull-down assay were further verified the DNA methylation state of the gene promoter region affected genes transcriptional activity after immunization with inactivated vaccine of Aeromonas salmonicida. Results A total of 8149 differentially methylated regions (DMRs) were screened, in which there were many immune-related genes with altered DNA methylation status. Meanwhile, 386 significantly differentially expressed genes (DEGs) were identified, many of which were significantly enriched in Toll-like receptor signaling pathway, NOD-like receptor signaling pathway and C-type lectin receptor signaling pathway. Combined analysis of WGBS results and RNA-seq results, a total of 9 DMRs of negatively regulated genes are located in the promoter region, including 2 hypermethylated genes with lower expression and 7 hypomethylated genes with higher expression. Then, two immune-related genes C5a anaphylatoxin chemotactic receptor 1-like (C5ar1-Like) and Eosinophil peroxidase-like (EPX-Like), were screened to explore the regulation mechanism of DNA methylation modification on their expression level. Moreover, the DNA methylation state of the gene promoter region affected ... |
format |
Dataset |
author |
Yingrui Li Lin Su Xiaofei Liu Huimin Guo Shun Zhou Yunji Xiu |
author_facet |
Yingrui Li Lin Su Xiaofei Liu Huimin Guo Shun Zhou Yunji Xiu |
author_sort |
Yingrui Li |
title |
Table_1_Immunity of turbot Induced by inactivated vaccine of Aeromonas salmonicida from the perspective of DNA methylation.docx |
title_short |
Table_1_Immunity of turbot Induced by inactivated vaccine of Aeromonas salmonicida from the perspective of DNA methylation.docx |
title_full |
Table_1_Immunity of turbot Induced by inactivated vaccine of Aeromonas salmonicida from the perspective of DNA methylation.docx |
title_fullStr |
Table_1_Immunity of turbot Induced by inactivated vaccine of Aeromonas salmonicida from the perspective of DNA methylation.docx |
title_full_unstemmed |
Table_1_Immunity of turbot Induced by inactivated vaccine of Aeromonas salmonicida from the perspective of DNA methylation.docx |
title_sort |
table_1_immunity of turbot induced by inactivated vaccine of aeromonas salmonicida from the perspective of dna methylation.docx |
publishDate |
2023 |
url |
https://doi.org/10.3389/fimmu.2023.1124322.s001 https://figshare.com/articles/dataset/Table_1_Immunity_of_turbot_Induced_by_inactivated_vaccine_of_Aeromonas_salmonicida_from_the_perspective_of_DNA_methylation_docx/22046741 |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_relation |
doi:10.3389/fimmu.2023.1124322.s001 https://figshare.com/articles/dataset/Table_1_Immunity_of_turbot_Induced_by_inactivated_vaccine_of_Aeromonas_salmonicida_from_the_perspective_of_DNA_methylation_docx/22046741 |
op_rights |
CC BY 4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fimmu.2023.1124322.s001 |
_version_ |
1766189008843440128 |