Image8_Atlantic cod (Gadus morhua) MHC I localizes to endolysosomal compartments independently of cytosolic sorting signals.tif
Major histocompatibility complex (MHC) class I and II are crucial for the adaptive immune system because they are involved in peptide presentation to T cells. Until recently, it was believed that MHC genes and their associated immune components had been conserved since their evolutionary emergence i...
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ftfrontimediafig:oai:figshare.com:article/21952088 2023-05-15T15:26:44+02:00 Image8_Atlantic cod (Gadus morhua) MHC I localizes to endolysosomal compartments independently of cytosolic sorting signals.tif Synne Arstad Bjørnestad Monica Hongrø Solbakken Kjetill S. Jakobsen Sissel Jentoft Oddmund Bakke Cinzia Progida 2023-01-25T04:41:25Z https://doi.org/10.3389/fcell.2023.1050323.s014 https://figshare.com/articles/figure/Image8_Atlantic_cod_Gadus_morhua_MHC_I_localizes_to_endolysosomal_compartments_independently_of_cytosolic_sorting_signals_tif/21952088 unknown doi:10.3389/fcell.2023.1050323.s014 https://figshare.com/articles/figure/Image8_Atlantic_cod_Gadus_morhua_MHC_I_localizes_to_endolysosomal_compartments_independently_of_cytosolic_sorting_signals_tif/21952088 CC BY 4.0 CC-BY Cell Biology Marine Biology Cell Development Proliferation and Death Cell Metabolism Cell Neurochemistry Cellular Interactions (incl. Adhesion Matrix Cell Wall) Atlantic cod (Gadus morhua) MHC I endolysosomes sorting signals cross-presentation Image Figure 2023 ftfrontimediafig https://doi.org/10.3389/fcell.2023.1050323.s014 2023-01-26T00:08:41Z Major histocompatibility complex (MHC) class I and II are crucial for the adaptive immune system because they are involved in peptide presentation to T cells. Until recently, it was believed that MHC genes and their associated immune components had been conserved since their evolutionary emergence in jawed fish. However, sequencing of the Atlantic cod (Gadus morhua) genome revealed a loss of MHC class II genes, and an extreme expansion of MHC class I genes. These findings lead to the hypothesis that a loss of the MHC class II pathway coincided with a more versatile use of MHC class I, but so far there is no direct experimental evidence in support of this. To gain a deeper understanding of the function of the expanded MHC class I, we selected five MHC class I gene variants representing five of the six clades identified in previous studies and investigated their intracellular localization in human and Atlantic cod larval cells. Intriguingly, we uncovered that all selected MHC class I variants localize to endolysosomal compartments in Atlantic cod cells. Additionally, by introducing point mutations or deletions in the cytosolic tail, we found that hypothetical sorting signals in the MHC class I cytosolic tail do not influence MHC class I trafficking. Moreover, we demonstrated that in Atlantic cod, tapasin and MHC class I colocalize on endolysosomes suggesting that peptide-loading assistance and stabilization of MHC class I occurs outside the endoplasmic reticulum. Altogether, our results demonstrate that MHC class I from Atlantic cod is sorted to the endolysosomal system, which may indicate that it interacts with exogenous peptides for potential cross presentation. Still Image atlantic cod Gadus morhua Frontiers: Figshare |
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Frontiers: Figshare |
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ftfrontimediafig |
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unknown |
topic |
Cell Biology Marine Biology Cell Development Proliferation and Death Cell Metabolism Cell Neurochemistry Cellular Interactions (incl. Adhesion Matrix Cell Wall) Atlantic cod (Gadus morhua) MHC I endolysosomes sorting signals cross-presentation |
spellingShingle |
Cell Biology Marine Biology Cell Development Proliferation and Death Cell Metabolism Cell Neurochemistry Cellular Interactions (incl. Adhesion Matrix Cell Wall) Atlantic cod (Gadus morhua) MHC I endolysosomes sorting signals cross-presentation Synne Arstad Bjørnestad Monica Hongrø Solbakken Kjetill S. Jakobsen Sissel Jentoft Oddmund Bakke Cinzia Progida Image8_Atlantic cod (Gadus morhua) MHC I localizes to endolysosomal compartments independently of cytosolic sorting signals.tif |
topic_facet |
Cell Biology Marine Biology Cell Development Proliferation and Death Cell Metabolism Cell Neurochemistry Cellular Interactions (incl. Adhesion Matrix Cell Wall) Atlantic cod (Gadus morhua) MHC I endolysosomes sorting signals cross-presentation |
description |
Major histocompatibility complex (MHC) class I and II are crucial for the adaptive immune system because they are involved in peptide presentation to T cells. Until recently, it was believed that MHC genes and their associated immune components had been conserved since their evolutionary emergence in jawed fish. However, sequencing of the Atlantic cod (Gadus morhua) genome revealed a loss of MHC class II genes, and an extreme expansion of MHC class I genes. These findings lead to the hypothesis that a loss of the MHC class II pathway coincided with a more versatile use of MHC class I, but so far there is no direct experimental evidence in support of this. To gain a deeper understanding of the function of the expanded MHC class I, we selected five MHC class I gene variants representing five of the six clades identified in previous studies and investigated their intracellular localization in human and Atlantic cod larval cells. Intriguingly, we uncovered that all selected MHC class I variants localize to endolysosomal compartments in Atlantic cod cells. Additionally, by introducing point mutations or deletions in the cytosolic tail, we found that hypothetical sorting signals in the MHC class I cytosolic tail do not influence MHC class I trafficking. Moreover, we demonstrated that in Atlantic cod, tapasin and MHC class I colocalize on endolysosomes suggesting that peptide-loading assistance and stabilization of MHC class I occurs outside the endoplasmic reticulum. Altogether, our results demonstrate that MHC class I from Atlantic cod is sorted to the endolysosomal system, which may indicate that it interacts with exogenous peptides for potential cross presentation. |
format |
Still Image |
author |
Synne Arstad Bjørnestad Monica Hongrø Solbakken Kjetill S. Jakobsen Sissel Jentoft Oddmund Bakke Cinzia Progida |
author_facet |
Synne Arstad Bjørnestad Monica Hongrø Solbakken Kjetill S. Jakobsen Sissel Jentoft Oddmund Bakke Cinzia Progida |
author_sort |
Synne Arstad Bjørnestad |
title |
Image8_Atlantic cod (Gadus morhua) MHC I localizes to endolysosomal compartments independently of cytosolic sorting signals.tif |
title_short |
Image8_Atlantic cod (Gadus morhua) MHC I localizes to endolysosomal compartments independently of cytosolic sorting signals.tif |
title_full |
Image8_Atlantic cod (Gadus morhua) MHC I localizes to endolysosomal compartments independently of cytosolic sorting signals.tif |
title_fullStr |
Image8_Atlantic cod (Gadus morhua) MHC I localizes to endolysosomal compartments independently of cytosolic sorting signals.tif |
title_full_unstemmed |
Image8_Atlantic cod (Gadus morhua) MHC I localizes to endolysosomal compartments independently of cytosolic sorting signals.tif |
title_sort |
image8_atlantic cod (gadus morhua) mhc i localizes to endolysosomal compartments independently of cytosolic sorting signals.tif |
publishDate |
2023 |
url |
https://doi.org/10.3389/fcell.2023.1050323.s014 https://figshare.com/articles/figure/Image8_Atlantic_cod_Gadus_morhua_MHC_I_localizes_to_endolysosomal_compartments_independently_of_cytosolic_sorting_signals_tif/21952088 |
genre |
atlantic cod Gadus morhua |
genre_facet |
atlantic cod Gadus morhua |
op_relation |
doi:10.3389/fcell.2023.1050323.s014 https://figshare.com/articles/figure/Image8_Atlantic_cod_Gadus_morhua_MHC_I_localizes_to_endolysosomal_compartments_independently_of_cytosolic_sorting_signals_tif/21952088 |
op_rights |
CC BY 4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fcell.2023.1050323.s014 |
_version_ |
1766357213500145664 |