DataSheet3_Atlantic cod (Gadus morhua) MHC I localizes to endolysosomal compartments independently of cytosolic sorting signals.FASTA
Major histocompatibility complex (MHC) class I and II are crucial for the adaptive immune system because they are involved in peptide presentation to T cells. Until recently, it was believed that MHC genes and their associated immune components had been conserved since their evolutionary emergence i...
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ftfrontimediafig:oai:figshare.com:article/21952046 2023-05-15T15:26:44+02:00 DataSheet3_Atlantic cod (Gadus morhua) MHC I localizes to endolysosomal compartments independently of cytosolic sorting signals.FASTA Synne Arstad Bjørnestad Monica Hongrø Solbakken Kjetill S. Jakobsen Sissel Jentoft Oddmund Bakke Cinzia Progida 2023-01-25T04:41:22Z https://doi.org/10.3389/fcell.2023.1050323.s002 https://figshare.com/articles/dataset/DataSheet3_Atlantic_cod_Gadus_morhua_MHC_I_localizes_to_endolysosomal_compartments_independently_of_cytosolic_sorting_signals_FASTA/21952046 unknown doi:10.3389/fcell.2023.1050323.s002 https://figshare.com/articles/dataset/DataSheet3_Atlantic_cod_Gadus_morhua_MHC_I_localizes_to_endolysosomal_compartments_independently_of_cytosolic_sorting_signals_FASTA/21952046 CC BY 4.0 CC-BY Cell Biology Marine Biology Cell Development Proliferation and Death Cell Metabolism Cell Neurochemistry Cellular Interactions (incl. Adhesion Matrix Cell Wall) Atlantic cod (Gadus morhua) MHC I endolysosomes sorting signals cross-presentation Dataset 2023 ftfrontimediafig https://doi.org/10.3389/fcell.2023.1050323.s002 2023-01-26T00:08:43Z Major histocompatibility complex (MHC) class I and II are crucial for the adaptive immune system because they are involved in peptide presentation to T cells. Until recently, it was believed that MHC genes and their associated immune components had been conserved since their evolutionary emergence in jawed fish. However, sequencing of the Atlantic cod (Gadus morhua) genome revealed a loss of MHC class II genes, and an extreme expansion of MHC class I genes. These findings lead to the hypothesis that a loss of the MHC class II pathway coincided with a more versatile use of MHC class I, but so far there is no direct experimental evidence in support of this. To gain a deeper understanding of the function of the expanded MHC class I, we selected five MHC class I gene variants representing five of the six clades identified in previous studies and investigated their intracellular localization in human and Atlantic cod larval cells. Intriguingly, we uncovered that all selected MHC class I variants localize to endolysosomal compartments in Atlantic cod cells. Additionally, by introducing point mutations or deletions in the cytosolic tail, we found that hypothetical sorting signals in the MHC class I cytosolic tail do not influence MHC class I trafficking. Moreover, we demonstrated that in Atlantic cod, tapasin and MHC class I colocalize on endolysosomes suggesting that peptide-loading assistance and stabilization of MHC class I occurs outside the endoplasmic reticulum. Altogether, our results demonstrate that MHC class I from Atlantic cod is sorted to the endolysosomal system, which may indicate that it interacts with exogenous peptides for potential cross presentation. Dataset atlantic cod Gadus morhua Frontiers: Figshare |
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Frontiers: Figshare |
op_collection_id |
ftfrontimediafig |
language |
unknown |
topic |
Cell Biology Marine Biology Cell Development Proliferation and Death Cell Metabolism Cell Neurochemistry Cellular Interactions (incl. Adhesion Matrix Cell Wall) Atlantic cod (Gadus morhua) MHC I endolysosomes sorting signals cross-presentation |
spellingShingle |
Cell Biology Marine Biology Cell Development Proliferation and Death Cell Metabolism Cell Neurochemistry Cellular Interactions (incl. Adhesion Matrix Cell Wall) Atlantic cod (Gadus morhua) MHC I endolysosomes sorting signals cross-presentation Synne Arstad Bjørnestad Monica Hongrø Solbakken Kjetill S. Jakobsen Sissel Jentoft Oddmund Bakke Cinzia Progida DataSheet3_Atlantic cod (Gadus morhua) MHC I localizes to endolysosomal compartments independently of cytosolic sorting signals.FASTA |
topic_facet |
Cell Biology Marine Biology Cell Development Proliferation and Death Cell Metabolism Cell Neurochemistry Cellular Interactions (incl. Adhesion Matrix Cell Wall) Atlantic cod (Gadus morhua) MHC I endolysosomes sorting signals cross-presentation |
description |
Major histocompatibility complex (MHC) class I and II are crucial for the adaptive immune system because they are involved in peptide presentation to T cells. Until recently, it was believed that MHC genes and their associated immune components had been conserved since their evolutionary emergence in jawed fish. However, sequencing of the Atlantic cod (Gadus morhua) genome revealed a loss of MHC class II genes, and an extreme expansion of MHC class I genes. These findings lead to the hypothesis that a loss of the MHC class II pathway coincided with a more versatile use of MHC class I, but so far there is no direct experimental evidence in support of this. To gain a deeper understanding of the function of the expanded MHC class I, we selected five MHC class I gene variants representing five of the six clades identified in previous studies and investigated their intracellular localization in human and Atlantic cod larval cells. Intriguingly, we uncovered that all selected MHC class I variants localize to endolysosomal compartments in Atlantic cod cells. Additionally, by introducing point mutations or deletions in the cytosolic tail, we found that hypothetical sorting signals in the MHC class I cytosolic tail do not influence MHC class I trafficking. Moreover, we demonstrated that in Atlantic cod, tapasin and MHC class I colocalize on endolysosomes suggesting that peptide-loading assistance and stabilization of MHC class I occurs outside the endoplasmic reticulum. Altogether, our results demonstrate that MHC class I from Atlantic cod is sorted to the endolysosomal system, which may indicate that it interacts with exogenous peptides for potential cross presentation. |
format |
Dataset |
author |
Synne Arstad Bjørnestad Monica Hongrø Solbakken Kjetill S. Jakobsen Sissel Jentoft Oddmund Bakke Cinzia Progida |
author_facet |
Synne Arstad Bjørnestad Monica Hongrø Solbakken Kjetill S. Jakobsen Sissel Jentoft Oddmund Bakke Cinzia Progida |
author_sort |
Synne Arstad Bjørnestad |
title |
DataSheet3_Atlantic cod (Gadus morhua) MHC I localizes to endolysosomal compartments independently of cytosolic sorting signals.FASTA |
title_short |
DataSheet3_Atlantic cod (Gadus morhua) MHC I localizes to endolysosomal compartments independently of cytosolic sorting signals.FASTA |
title_full |
DataSheet3_Atlantic cod (Gadus morhua) MHC I localizes to endolysosomal compartments independently of cytosolic sorting signals.FASTA |
title_fullStr |
DataSheet3_Atlantic cod (Gadus morhua) MHC I localizes to endolysosomal compartments independently of cytosolic sorting signals.FASTA |
title_full_unstemmed |
DataSheet3_Atlantic cod (Gadus morhua) MHC I localizes to endolysosomal compartments independently of cytosolic sorting signals.FASTA |
title_sort |
datasheet3_atlantic cod (gadus morhua) mhc i localizes to endolysosomal compartments independently of cytosolic sorting signals.fasta |
publishDate |
2023 |
url |
https://doi.org/10.3389/fcell.2023.1050323.s002 https://figshare.com/articles/dataset/DataSheet3_Atlantic_cod_Gadus_morhua_MHC_I_localizes_to_endolysosomal_compartments_independently_of_cytosolic_sorting_signals_FASTA/21952046 |
genre |
atlantic cod Gadus morhua |
genre_facet |
atlantic cod Gadus morhua |
op_relation |
doi:10.3389/fcell.2023.1050323.s002 https://figshare.com/articles/dataset/DataSheet3_Atlantic_cod_Gadus_morhua_MHC_I_localizes_to_endolysosomal_compartments_independently_of_cytosolic_sorting_signals_FASTA/21952046 |
op_rights |
CC BY 4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fcell.2023.1050323.s002 |
_version_ |
1766357215531237376 |