Data_Sheet_1_Elevated CO2 reduces copper accumulation and toxicity in the diatom Thalassiosira pseudonana.PDF
The projected ocean acidification (OA) associated with increasing atmospheric CO 2 alters seawater chemistry and hence the bio-toxicity of metal ions. However, it is still unclear how OA might affect the long-term resilience of globally important marine microalgae to anthropogenic metal stress. To e...
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ftfrontimediafig:oai:figshare.com:article/21827394 2024-09-15T18:27:57+00:00 Data_Sheet_1_Elevated CO2 reduces copper accumulation and toxicity in the diatom Thalassiosira pseudonana.PDF Dong Xu Shujie Huang Xiao Fan Xiaowen Zhang Yitao Wang Wei Wang John Beardall Georgina Brennan Naihao Ye 2023-01-06T06:11:00Z https://doi.org/10.3389/fmicb.2022.1113388.s001 https://figshare.com/articles/dataset/Data_Sheet_1_Elevated_CO2_reduces_copper_accumulation_and_toxicity_in_the_diatom_Thalassiosira_pseudonana_PDF/21827394 unknown doi:10.3389/fmicb.2022.1113388.s001 https://figshare.com/articles/dataset/Data_Sheet_1_Elevated_CO2_reduces_copper_accumulation_and_toxicity_in_the_diatom_Thalassiosira_pseudonana_PDF/21827394 CC BY 4.0 Microbiology Microbial Genetics Microbial Ecology Mycology ocean acidification copper accumulation copper toxicity adaptation Thalassiosira pseudonana Dataset 2023 ftfrontimediafig https://doi.org/10.3389/fmicb.2022.1113388.s001 2024-08-19T06:19:59Z The projected ocean acidification (OA) associated with increasing atmospheric CO 2 alters seawater chemistry and hence the bio-toxicity of metal ions. However, it is still unclear how OA might affect the long-term resilience of globally important marine microalgae to anthropogenic metal stress. To explore the effect of increasing pCO 2 on copper metabolism in the diatom Thalassiosira pseudonana (CCMP 1335), we employed an integrated eco-physiological, analytical chemistry, and transcriptomic approach to clarify the effect of increasing pCO 2 on copper metabolism of Thalassiosira pseudonana across different temporal (short-term vs. long-term) and spatial (indoor laboratory experiments vs. outdoor mesocosms experiments) scales. We found that increasing pCO 2 (1,000 and 2,000 μatm) promoted growth and photosynthesis, but decreased copper accumulation and alleviated its bio-toxicity to T. pseudonana. Transcriptomics results indicated that T. pseudonana altered the copper detoxification strategy under OA by decreasing copper uptake and enhancing copper-thiol complexation and copper efflux. Biochemical analysis further showed that the activities of the antioxidant enzymes glutathione peroxidase (GPX), catalase (CAT), and phytochelatin synthetase (PCS) were enhanced to mitigate oxidative damage of copper stress under elevated CO 2 . Our results provide a basis for a better understanding of the bioremediation capacity of marine primary producers, which may have profound effect on the security of seafood quality and marine ecosystem sustainability under further climate change. Dataset Ocean acidification Frontiers: Figshare |
institution |
Open Polar |
collection |
Frontiers: Figshare |
op_collection_id |
ftfrontimediafig |
language |
unknown |
topic |
Microbiology Microbial Genetics Microbial Ecology Mycology ocean acidification copper accumulation copper toxicity adaptation Thalassiosira pseudonana |
spellingShingle |
Microbiology Microbial Genetics Microbial Ecology Mycology ocean acidification copper accumulation copper toxicity adaptation Thalassiosira pseudonana Dong Xu Shujie Huang Xiao Fan Xiaowen Zhang Yitao Wang Wei Wang John Beardall Georgina Brennan Naihao Ye Data_Sheet_1_Elevated CO2 reduces copper accumulation and toxicity in the diatom Thalassiosira pseudonana.PDF |
topic_facet |
Microbiology Microbial Genetics Microbial Ecology Mycology ocean acidification copper accumulation copper toxicity adaptation Thalassiosira pseudonana |
description |
The projected ocean acidification (OA) associated with increasing atmospheric CO 2 alters seawater chemistry and hence the bio-toxicity of metal ions. However, it is still unclear how OA might affect the long-term resilience of globally important marine microalgae to anthropogenic metal stress. To explore the effect of increasing pCO 2 on copper metabolism in the diatom Thalassiosira pseudonana (CCMP 1335), we employed an integrated eco-physiological, analytical chemistry, and transcriptomic approach to clarify the effect of increasing pCO 2 on copper metabolism of Thalassiosira pseudonana across different temporal (short-term vs. long-term) and spatial (indoor laboratory experiments vs. outdoor mesocosms experiments) scales. We found that increasing pCO 2 (1,000 and 2,000 μatm) promoted growth and photosynthesis, but decreased copper accumulation and alleviated its bio-toxicity to T. pseudonana. Transcriptomics results indicated that T. pseudonana altered the copper detoxification strategy under OA by decreasing copper uptake and enhancing copper-thiol complexation and copper efflux. Biochemical analysis further showed that the activities of the antioxidant enzymes glutathione peroxidase (GPX), catalase (CAT), and phytochelatin synthetase (PCS) were enhanced to mitigate oxidative damage of copper stress under elevated CO 2 . Our results provide a basis for a better understanding of the bioremediation capacity of marine primary producers, which may have profound effect on the security of seafood quality and marine ecosystem sustainability under further climate change. |
format |
Dataset |
author |
Dong Xu Shujie Huang Xiao Fan Xiaowen Zhang Yitao Wang Wei Wang John Beardall Georgina Brennan Naihao Ye |
author_facet |
Dong Xu Shujie Huang Xiao Fan Xiaowen Zhang Yitao Wang Wei Wang John Beardall Georgina Brennan Naihao Ye |
author_sort |
Dong Xu |
title |
Data_Sheet_1_Elevated CO2 reduces copper accumulation and toxicity in the diatom Thalassiosira pseudonana.PDF |
title_short |
Data_Sheet_1_Elevated CO2 reduces copper accumulation and toxicity in the diatom Thalassiosira pseudonana.PDF |
title_full |
Data_Sheet_1_Elevated CO2 reduces copper accumulation and toxicity in the diatom Thalassiosira pseudonana.PDF |
title_fullStr |
Data_Sheet_1_Elevated CO2 reduces copper accumulation and toxicity in the diatom Thalassiosira pseudonana.PDF |
title_full_unstemmed |
Data_Sheet_1_Elevated CO2 reduces copper accumulation and toxicity in the diatom Thalassiosira pseudonana.PDF |
title_sort |
data_sheet_1_elevated co2 reduces copper accumulation and toxicity in the diatom thalassiosira pseudonana.pdf |
publishDate |
2023 |
url |
https://doi.org/10.3389/fmicb.2022.1113388.s001 https://figshare.com/articles/dataset/Data_Sheet_1_Elevated_CO2_reduces_copper_accumulation_and_toxicity_in_the_diatom_Thalassiosira_pseudonana_PDF/21827394 |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_relation |
doi:10.3389/fmicb.2022.1113388.s001 https://figshare.com/articles/dataset/Data_Sheet_1_Elevated_CO2_reduces_copper_accumulation_and_toxicity_in_the_diatom_Thalassiosira_pseudonana_PDF/21827394 |
op_rights |
CC BY 4.0 |
op_doi |
https://doi.org/10.3389/fmicb.2022.1113388.s001 |
_version_ |
1810469239401742336 |