Data_Sheet_1_Elevated CO2 reduces copper accumulation and toxicity in the diatom Thalassiosira pseudonana.PDF

The projected ocean acidification (OA) associated with increasing atmospheric CO 2 alters seawater chemistry and hence the bio-toxicity of metal ions. However, it is still unclear how OA might affect the long-term resilience of globally important marine microalgae to anthropogenic metal stress. To e...

Full description

Bibliographic Details
Main Authors: Dong Xu, Shujie Huang, Xiao Fan, Xiaowen Zhang, Yitao Wang, Wei Wang, John Beardall, Georgina Brennan, Naihao Ye
Format: Dataset
Language:unknown
Published: 2023
Subjects:
Microbiology
Microbial Genetics
Microbial Ecology
Mycology
ocean acidification
copper accumulation
copper toxicity
adaptation
Thalassiosira pseudonana
Ocean acidification
Online Access:https://doi.org/10.3389/fmicb.2022.1113388.s001
https://figshare.com/articles/dataset/Data_Sheet_1_Elevated_CO2_reduces_copper_accumulation_and_toxicity_in_the_diatom_Thalassiosira_pseudonana_PDF/21827394
Description
Summary:The projected ocean acidification (OA) associated with increasing atmospheric CO 2 alters seawater chemistry and hence the bio-toxicity of metal ions. However, it is still unclear how OA might affect the long-term resilience of globally important marine microalgae to anthropogenic metal stress. To explore the effect of increasing pCO 2 on copper metabolism in the diatom Thalassiosira pseudonana (CCMP 1335), we employed an integrated eco-physiological, analytical chemistry, and transcriptomic approach to clarify the effect of increasing pCO 2 on copper metabolism of Thalassiosira pseudonana across different temporal (short-term vs. long-term) and spatial (indoor laboratory experiments vs. outdoor mesocosms experiments) scales. We found that increasing pCO 2 (1,000 and 2,000 μatm) promoted growth and photosynthesis, but decreased copper accumulation and alleviated its bio-toxicity to T. pseudonana. Transcriptomics results indicated that T. pseudonana altered the copper detoxification strategy under OA by decreasing copper uptake and enhancing copper-thiol complexation and copper efflux. Biochemical analysis further showed that the activities of the antioxidant enzymes glutathione peroxidase (GPX), catalase (CAT), and phytochelatin synthetase (PCS) were enhanced to mitigate oxidative damage of copper stress under elevated CO 2 . Our results provide a basis for a better understanding of the bioremediation capacity of marine primary producers, which may have profound effect on the security of seafood quality and marine ecosystem sustainability under further climate change.

Similar Items