Data_Sheet_1_Coastal water bacteriophages infect various sets of Vibrio parahaemolyticus sequence types.zip
Introduction Gastrointestinal illnesses associated with the consumption of shellfish contaminated with Vibrio parahaemolyticus have a negative impact on the shellfish industry due to recalls and loss of consumer confidence in products. This bacterial pathogen is very diverse and specific sequence ty...
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ftfrontimediafig:oai:figshare.com:article/21748703 2023-05-15T17:41:33+02:00 Data_Sheet_1_Coastal water bacteriophages infect various sets of Vibrio parahaemolyticus sequence types.zip Kari A. Brossard Stoos Jennifer Ren Robin R. Shields-Cutler Kelly L. Sams Shannon Caldwell Marvin B. Ho Gregg Rivara Cheryl A. Whistler Stephen H. Jones Martin Wiedmann Jamie DeMent Rodman G. Getchell Hélène Marquis 2022-12-19T04:14:31Z https://doi.org/10.3389/fmicb.2022.1041942.s001 https://figshare.com/articles/dataset/Data_Sheet_1_Coastal_water_bacteriophages_infect_various_sets_of_Vibrio_parahaemolyticus_sequence_types_zip/21748703 unknown doi:10.3389/fmicb.2022.1041942.s001 https://figshare.com/articles/dataset/Data_Sheet_1_Coastal_water_bacteriophages_infect_various_sets_of_Vibrio_parahaemolyticus_sequence_types_zip/21748703 CC BY 4.0 CC-BY Microbiology Microbial Genetics Microbial Ecology Mycology Vibrio parahaemolyticus vibriophage sequence type phage ST36 Dataset 2022 ftfrontimediafig https://doi.org/10.3389/fmicb.2022.1041942.s001 2022-12-22T00:09:38Z Introduction Gastrointestinal illnesses associated with the consumption of shellfish contaminated with Vibrio parahaemolyticus have a negative impact on the shellfish industry due to recalls and loss of consumer confidence in products. This bacterial pathogen is very diverse and specific sequence types (STs), ST631 and ST36, have emerged as prevalent causes of Vibrio foodborne disease outbreaks in the US, though other STs have been implicated in sporadic cases. We investigated whether bacteriophages could be used as a proxy to monitor for the presence of distinct V. parahaemolyticus STs in coastal waters. Methods For this purpose, bacteriophages infecting V. parahaemolyticus were isolated from water samples collected on the Northeast Atlantic coast. The isolated phages were tested against a collection of 29 V. parahaemolyticus isolates representing 18 STs, including six clonal complexes (CC). Four distinct phages were identified based on their ability to infect different sets of V. parahaemolyticus isolates. Results and Discussion Overall, the 29 bacterial isolates segregated into one of eight patterns of susceptibility, ranging from resistance to all four phages to susceptibility to any number of phages. STs represented by more than one bacterial isolate segregated within the same pattern of susceptibility except for one V. parahaemolyticus ST. Other patterns of susceptibility included exclusively clinical isolates represented by distinct STs. Overall, this study suggests that phages populating coastal waters could be exploited to monitor for the presence of V. parahaemolyticus STs known to cause foodborne outbreaks. Dataset Northeast Atlantic Frontiers: Figshare |
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Frontiers: Figshare |
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ftfrontimediafig |
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unknown |
topic |
Microbiology Microbial Genetics Microbial Ecology Mycology Vibrio parahaemolyticus vibriophage sequence type phage ST36 |
spellingShingle |
Microbiology Microbial Genetics Microbial Ecology Mycology Vibrio parahaemolyticus vibriophage sequence type phage ST36 Kari A. Brossard Stoos Jennifer Ren Robin R. Shields-Cutler Kelly L. Sams Shannon Caldwell Marvin B. Ho Gregg Rivara Cheryl A. Whistler Stephen H. Jones Martin Wiedmann Jamie DeMent Rodman G. Getchell Hélène Marquis Data_Sheet_1_Coastal water bacteriophages infect various sets of Vibrio parahaemolyticus sequence types.zip |
topic_facet |
Microbiology Microbial Genetics Microbial Ecology Mycology Vibrio parahaemolyticus vibriophage sequence type phage ST36 |
description |
Introduction Gastrointestinal illnesses associated with the consumption of shellfish contaminated with Vibrio parahaemolyticus have a negative impact on the shellfish industry due to recalls and loss of consumer confidence in products. This bacterial pathogen is very diverse and specific sequence types (STs), ST631 and ST36, have emerged as prevalent causes of Vibrio foodborne disease outbreaks in the US, though other STs have been implicated in sporadic cases. We investigated whether bacteriophages could be used as a proxy to monitor for the presence of distinct V. parahaemolyticus STs in coastal waters. Methods For this purpose, bacteriophages infecting V. parahaemolyticus were isolated from water samples collected on the Northeast Atlantic coast. The isolated phages were tested against a collection of 29 V. parahaemolyticus isolates representing 18 STs, including six clonal complexes (CC). Four distinct phages were identified based on their ability to infect different sets of V. parahaemolyticus isolates. Results and Discussion Overall, the 29 bacterial isolates segregated into one of eight patterns of susceptibility, ranging from resistance to all four phages to susceptibility to any number of phages. STs represented by more than one bacterial isolate segregated within the same pattern of susceptibility except for one V. parahaemolyticus ST. Other patterns of susceptibility included exclusively clinical isolates represented by distinct STs. Overall, this study suggests that phages populating coastal waters could be exploited to monitor for the presence of V. parahaemolyticus STs known to cause foodborne outbreaks. |
format |
Dataset |
author |
Kari A. Brossard Stoos Jennifer Ren Robin R. Shields-Cutler Kelly L. Sams Shannon Caldwell Marvin B. Ho Gregg Rivara Cheryl A. Whistler Stephen H. Jones Martin Wiedmann Jamie DeMent Rodman G. Getchell Hélène Marquis |
author_facet |
Kari A. Brossard Stoos Jennifer Ren Robin R. Shields-Cutler Kelly L. Sams Shannon Caldwell Marvin B. Ho Gregg Rivara Cheryl A. Whistler Stephen H. Jones Martin Wiedmann Jamie DeMent Rodman G. Getchell Hélène Marquis |
author_sort |
Kari A. Brossard Stoos |
title |
Data_Sheet_1_Coastal water bacteriophages infect various sets of Vibrio parahaemolyticus sequence types.zip |
title_short |
Data_Sheet_1_Coastal water bacteriophages infect various sets of Vibrio parahaemolyticus sequence types.zip |
title_full |
Data_Sheet_1_Coastal water bacteriophages infect various sets of Vibrio parahaemolyticus sequence types.zip |
title_fullStr |
Data_Sheet_1_Coastal water bacteriophages infect various sets of Vibrio parahaemolyticus sequence types.zip |
title_full_unstemmed |
Data_Sheet_1_Coastal water bacteriophages infect various sets of Vibrio parahaemolyticus sequence types.zip |
title_sort |
data_sheet_1_coastal water bacteriophages infect various sets of vibrio parahaemolyticus sequence types.zip |
publishDate |
2022 |
url |
https://doi.org/10.3389/fmicb.2022.1041942.s001 https://figshare.com/articles/dataset/Data_Sheet_1_Coastal_water_bacteriophages_infect_various_sets_of_Vibrio_parahaemolyticus_sequence_types_zip/21748703 |
genre |
Northeast Atlantic |
genre_facet |
Northeast Atlantic |
op_relation |
doi:10.3389/fmicb.2022.1041942.s001 https://figshare.com/articles/dataset/Data_Sheet_1_Coastal_water_bacteriophages_infect_various_sets_of_Vibrio_parahaemolyticus_sequence_types_zip/21748703 |
op_rights |
CC BY 4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fmicb.2022.1041942.s001 |
_version_ |
1766143161487327232 |