DataSheet1_Investigating the relationship between the skin microbiome and flame retardant exposure of the endangered St. Lawrence Estuary beluga.zip
The endangered beluga (Delphinapterus leucas) population in the St. Lawrence Estuary (SLE) in eastern Canada, the largest estuary in the world, is declining. Elevated tissue concentrations of a wide range of environmental contaminants, for example, halogenated flame retardants (HFRs) including polyb...
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Online Access: | https://doi.org/10.3389/fenvs.2022.954060.s001 https://figshare.com/articles/dataset/DataSheet1_Investigating_the_relationship_between_the_skin_microbiome_and_flame_retardant_exposure_of_the_endangered_St_Lawrence_Estuary_beluga_zip/21352773 |
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ftfrontimediafig:oai:figshare.com:article/21352773 2023-05-15T15:41:31+02:00 DataSheet1_Investigating the relationship between the skin microbiome and flame retardant exposure of the endangered St. Lawrence Estuary beluga.zip Baofeng Jia Emma Garlock Michael J. Allison Robert Michaud Raymond Lo Jessica M. Round Caren C. Helbing Jonathan Verreault Fiona S. L. Brinkman 2022-10-18T05:21:35Z https://doi.org/10.3389/fenvs.2022.954060.s001 https://figshare.com/articles/dataset/DataSheet1_Investigating_the_relationship_between_the_skin_microbiome_and_flame_retardant_exposure_of_the_endangered_St_Lawrence_Estuary_beluga_zip/21352773 unknown doi:10.3389/fenvs.2022.954060.s001 https://figshare.com/articles/dataset/DataSheet1_Investigating_the_relationship_between_the_skin_microbiome_and_flame_retardant_exposure_of_the_endangered_St_Lawrence_Estuary_beluga_zip/21352773 CC BY 4.0 CC-BY Environmental Science Climate Science Environmental Impact Assessment Environmental Management Soil Biology Water Treatment Processes Environmental Engineering Design Environmental Engineering Modelling Environmental Technologies bacteria belugas Delphinapterus leucas halogenated flame retardants metagenomics functional microbiome St. Lawrence Estuary skins swab Dataset 2022 ftfrontimediafig https://doi.org/10.3389/fenvs.2022.954060.s001 2022-10-19T23:07:17Z The endangered beluga (Delphinapterus leucas) population in the St. Lawrence Estuary (SLE) in eastern Canada, the largest estuary in the world, is declining. Elevated tissue concentrations of a wide range of environmental contaminants, for example, halogenated flame retardants (HFRs) including polybrominated diphenyl ethers (PBDEs), might play a role in the non-recovery of this population. In mammals, HFRs have been reported to impair the metabolic regulation, including amino acid and fatty acid pathways. In the present study, we collected both blubber and skin swab samples from tissue biopsies from 56 adult SLE belugas and analyzed their blubber for the concentrations of a comprehensive suite of PBDEs and other HFRs. Using 16S rRNA marker and shotgun metagenomic approaches using skin swabs, we investigated, for the first time, the SLE beluga skin microbiome and the SLE water microbiome, providing valuable comparative taxonomic and functional microbiome information. We found that belugas have a unique skin microbiome that is distinct from surrounding SLE water, regardless of the beluga sex or location in the SLE. We further characterized the core microbiome of SLE beluga skin and surrounding SLE water, and identified bacterial taxa and gene functional pathways associated with the skin microbiome that correlated with beluga blubber HFR concentrations. Namely, we identified the phylum Nitrospinae and candidate phylum PAUC34f as potential taxa of interest that are associated with blubber HFR concentrations. We hypothesize that the biodegradation of HFRs within the beluga blubber and skin results in an increase in local metabolite concentrations that leads to the proliferation of Nitrospinae and PAUC32f. This work demonstrates the utility of studying the core microbiome of the SLE beluga skin using a swab method that could be adapted to field sampling. Further studies of the temporal effects of contaminant exposure on SLE beluga skin and SLE water microbiomes is warranted for potentially better monitoring and ... Dataset Beluga Beluga* Delphinapterus leucas Frontiers: Figshare Canada |
institution |
Open Polar |
collection |
Frontiers: Figshare |
op_collection_id |
ftfrontimediafig |
language |
unknown |
topic |
Environmental Science Climate Science Environmental Impact Assessment Environmental Management Soil Biology Water Treatment Processes Environmental Engineering Design Environmental Engineering Modelling Environmental Technologies bacteria belugas Delphinapterus leucas halogenated flame retardants metagenomics functional microbiome St. Lawrence Estuary skins swab |
spellingShingle |
Environmental Science Climate Science Environmental Impact Assessment Environmental Management Soil Biology Water Treatment Processes Environmental Engineering Design Environmental Engineering Modelling Environmental Technologies bacteria belugas Delphinapterus leucas halogenated flame retardants metagenomics functional microbiome St. Lawrence Estuary skins swab Baofeng Jia Emma Garlock Michael J. Allison Robert Michaud Raymond Lo Jessica M. Round Caren C. Helbing Jonathan Verreault Fiona S. L. Brinkman DataSheet1_Investigating the relationship between the skin microbiome and flame retardant exposure of the endangered St. Lawrence Estuary beluga.zip |
topic_facet |
Environmental Science Climate Science Environmental Impact Assessment Environmental Management Soil Biology Water Treatment Processes Environmental Engineering Design Environmental Engineering Modelling Environmental Technologies bacteria belugas Delphinapterus leucas halogenated flame retardants metagenomics functional microbiome St. Lawrence Estuary skins swab |
description |
The endangered beluga (Delphinapterus leucas) population in the St. Lawrence Estuary (SLE) in eastern Canada, the largest estuary in the world, is declining. Elevated tissue concentrations of a wide range of environmental contaminants, for example, halogenated flame retardants (HFRs) including polybrominated diphenyl ethers (PBDEs), might play a role in the non-recovery of this population. In mammals, HFRs have been reported to impair the metabolic regulation, including amino acid and fatty acid pathways. In the present study, we collected both blubber and skin swab samples from tissue biopsies from 56 adult SLE belugas and analyzed their blubber for the concentrations of a comprehensive suite of PBDEs and other HFRs. Using 16S rRNA marker and shotgun metagenomic approaches using skin swabs, we investigated, for the first time, the SLE beluga skin microbiome and the SLE water microbiome, providing valuable comparative taxonomic and functional microbiome information. We found that belugas have a unique skin microbiome that is distinct from surrounding SLE water, regardless of the beluga sex or location in the SLE. We further characterized the core microbiome of SLE beluga skin and surrounding SLE water, and identified bacterial taxa and gene functional pathways associated with the skin microbiome that correlated with beluga blubber HFR concentrations. Namely, we identified the phylum Nitrospinae and candidate phylum PAUC34f as potential taxa of interest that are associated with blubber HFR concentrations. We hypothesize that the biodegradation of HFRs within the beluga blubber and skin results in an increase in local metabolite concentrations that leads to the proliferation of Nitrospinae and PAUC32f. This work demonstrates the utility of studying the core microbiome of the SLE beluga skin using a swab method that could be adapted to field sampling. Further studies of the temporal effects of contaminant exposure on SLE beluga skin and SLE water microbiomes is warranted for potentially better monitoring and ... |
format |
Dataset |
author |
Baofeng Jia Emma Garlock Michael J. Allison Robert Michaud Raymond Lo Jessica M. Round Caren C. Helbing Jonathan Verreault Fiona S. L. Brinkman |
author_facet |
Baofeng Jia Emma Garlock Michael J. Allison Robert Michaud Raymond Lo Jessica M. Round Caren C. Helbing Jonathan Verreault Fiona S. L. Brinkman |
author_sort |
Baofeng Jia |
title |
DataSheet1_Investigating the relationship between the skin microbiome and flame retardant exposure of the endangered St. Lawrence Estuary beluga.zip |
title_short |
DataSheet1_Investigating the relationship between the skin microbiome and flame retardant exposure of the endangered St. Lawrence Estuary beluga.zip |
title_full |
DataSheet1_Investigating the relationship between the skin microbiome and flame retardant exposure of the endangered St. Lawrence Estuary beluga.zip |
title_fullStr |
DataSheet1_Investigating the relationship between the skin microbiome and flame retardant exposure of the endangered St. Lawrence Estuary beluga.zip |
title_full_unstemmed |
DataSheet1_Investigating the relationship between the skin microbiome and flame retardant exposure of the endangered St. Lawrence Estuary beluga.zip |
title_sort |
datasheet1_investigating the relationship between the skin microbiome and flame retardant exposure of the endangered st. lawrence estuary beluga.zip |
publishDate |
2022 |
url |
https://doi.org/10.3389/fenvs.2022.954060.s001 https://figshare.com/articles/dataset/DataSheet1_Investigating_the_relationship_between_the_skin_microbiome_and_flame_retardant_exposure_of_the_endangered_St_Lawrence_Estuary_beluga_zip/21352773 |
geographic |
Canada |
geographic_facet |
Canada |
genre |
Beluga Beluga* Delphinapterus leucas |
genre_facet |
Beluga Beluga* Delphinapterus leucas |
op_relation |
doi:10.3389/fenvs.2022.954060.s001 https://figshare.com/articles/dataset/DataSheet1_Investigating_the_relationship_between_the_skin_microbiome_and_flame_retardant_exposure_of_the_endangered_St_Lawrence_Estuary_beluga_zip/21352773 |
op_rights |
CC BY 4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fenvs.2022.954060.s001 |
_version_ |
1766374402150105088 |