Table_1_Proton gradients across the coral calcifying cell layer: Effects of light, ocean acidification and carbonate chemistry.docx
In corals, pH regulation of the extracellular calcifying medium (ECM) by the calcifying cell layer is a crucial step in the calcification process and is potentially important to influencing how corals respond to ocean acidification. Here, we analyzed the growing edge of the reef coral Stylophora pis...
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ftfrontimediafig:oai:figshare.com:article/21194413 2024-09-15T18:27:43+00:00 Table_1_Proton gradients across the coral calcifying cell layer: Effects of light, ocean acidification and carbonate chemistry.docx Alexander A. Venn Eric Tambutté Steeve Comeau Sylvie Tambutté 2022-09-23T06:15:24Z https://doi.org/10.3389/fmars.2022.973908.s004 https://figshare.com/articles/dataset/Table_1_Proton_gradients_across_the_coral_calcifying_cell_layer_Effects_of_light_ocean_acidification_and_carbonate_chemistry_docx/21194413 unknown doi:10.3389/fmars.2022.973908.s004 https://figshare.com/articles/dataset/Table_1_Proton_gradients_across_the_coral_calcifying_cell_layer_Effects_of_light_ocean_acidification_and_carbonate_chemistry_docx/21194413 CC BY 4.0 Oceanography Marine Biology Marine Geoscience Biological Oceanography Chemical Oceanography Physical Oceanography Marine Engineering pH regulation biomineralization scleractinian physiology climate-change Dataset 2022 ftfrontimediafig https://doi.org/10.3389/fmars.2022.973908.s004 2024-08-19T06:19:49Z In corals, pH regulation of the extracellular calcifying medium (ECM) by the calcifying cell layer is a crucial step in the calcification process and is potentially important to influencing how corals respond to ocean acidification. Here, we analyzed the growing edge of the reef coral Stylophora pistillata to make the first characterization of the proton gradient across the coral calcifying epithelium. At seawater pH 8 we found that while the calcifying epithelium elevates pH in the ECM on its apical side above that of seawater, pH on its basal side in the mesoglea is markedly lower, highlighting that the calcifying cells are exposed to a microenvironment distinct from the external environment. Coral symbiont photosynthesis elevates pH in the mesoglea, but experimental ocean acidification and decreased seawater inorganic carbon concentration lead to large declines in mesoglea pH relative to the ECM, which is maintained relatively stable. Together, our results indicate that the coral calcifying epithelium is functionally polarized and that environmental variation impacts pH ECM regulation through its effects on the basal side of the calcifying cells. Dataset Ocean acidification Frontiers: Figshare |
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Open Polar |
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Frontiers: Figshare |
op_collection_id |
ftfrontimediafig |
language |
unknown |
topic |
Oceanography Marine Biology Marine Geoscience Biological Oceanography Chemical Oceanography Physical Oceanography Marine Engineering pH regulation biomineralization scleractinian physiology climate-change |
spellingShingle |
Oceanography Marine Biology Marine Geoscience Biological Oceanography Chemical Oceanography Physical Oceanography Marine Engineering pH regulation biomineralization scleractinian physiology climate-change Alexander A. Venn Eric Tambutté Steeve Comeau Sylvie Tambutté Table_1_Proton gradients across the coral calcifying cell layer: Effects of light, ocean acidification and carbonate chemistry.docx |
topic_facet |
Oceanography Marine Biology Marine Geoscience Biological Oceanography Chemical Oceanography Physical Oceanography Marine Engineering pH regulation biomineralization scleractinian physiology climate-change |
description |
In corals, pH regulation of the extracellular calcifying medium (ECM) by the calcifying cell layer is a crucial step in the calcification process and is potentially important to influencing how corals respond to ocean acidification. Here, we analyzed the growing edge of the reef coral Stylophora pistillata to make the first characterization of the proton gradient across the coral calcifying epithelium. At seawater pH 8 we found that while the calcifying epithelium elevates pH in the ECM on its apical side above that of seawater, pH on its basal side in the mesoglea is markedly lower, highlighting that the calcifying cells are exposed to a microenvironment distinct from the external environment. Coral symbiont photosynthesis elevates pH in the mesoglea, but experimental ocean acidification and decreased seawater inorganic carbon concentration lead to large declines in mesoglea pH relative to the ECM, which is maintained relatively stable. Together, our results indicate that the coral calcifying epithelium is functionally polarized and that environmental variation impacts pH ECM regulation through its effects on the basal side of the calcifying cells. |
format |
Dataset |
author |
Alexander A. Venn Eric Tambutté Steeve Comeau Sylvie Tambutté |
author_facet |
Alexander A. Venn Eric Tambutté Steeve Comeau Sylvie Tambutté |
author_sort |
Alexander A. Venn |
title |
Table_1_Proton gradients across the coral calcifying cell layer: Effects of light, ocean acidification and carbonate chemistry.docx |
title_short |
Table_1_Proton gradients across the coral calcifying cell layer: Effects of light, ocean acidification and carbonate chemistry.docx |
title_full |
Table_1_Proton gradients across the coral calcifying cell layer: Effects of light, ocean acidification and carbonate chemistry.docx |
title_fullStr |
Table_1_Proton gradients across the coral calcifying cell layer: Effects of light, ocean acidification and carbonate chemistry.docx |
title_full_unstemmed |
Table_1_Proton gradients across the coral calcifying cell layer: Effects of light, ocean acidification and carbonate chemistry.docx |
title_sort |
table_1_proton gradients across the coral calcifying cell layer: effects of light, ocean acidification and carbonate chemistry.docx |
publishDate |
2022 |
url |
https://doi.org/10.3389/fmars.2022.973908.s004 https://figshare.com/articles/dataset/Table_1_Proton_gradients_across_the_coral_calcifying_cell_layer_Effects_of_light_ocean_acidification_and_carbonate_chemistry_docx/21194413 |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_relation |
doi:10.3389/fmars.2022.973908.s004 https://figshare.com/articles/dataset/Table_1_Proton_gradients_across_the_coral_calcifying_cell_layer_Effects_of_light_ocean_acidification_and_carbonate_chemistry_docx/21194413 |
op_rights |
CC BY 4.0 |
op_doi |
https://doi.org/10.3389/fmars.2022.973908.s004 |
_version_ |
1810468957944020992 |