Table_2_Molecular cloning, characterization, and expression of two 5-HTRs from the pearl oyster Pinctada fucata martensii.xlsx
The receptors of serotonin, also known as 5-hydroxytryptamine receptor (5-HTR) can mediate regulatory metamorphosis processes in a variety of mollusks. Studying the mechanisms of metamorphosis of the pearl oyster is significant to elucidate breeding, resource recovery and marine pearl production. In...
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ftfrontimediafig:oai:figshare.com:article/21193672 2023-05-15T15:58:51+02:00 Table_2_Molecular cloning, characterization, and expression of two 5-HTRs from the pearl oyster Pinctada fucata martensii.xlsx Shaojie Zhu Yubo He Qiongyu Xu Jiabin Zhang Chuangye Yang Ruijuan Hao Junhui Li Yuewen Deng 2022-09-23T05:22:53Z https://doi.org/10.3389/fmars.2022.1011420.s002 https://figshare.com/articles/dataset/Table_2_Molecular_cloning_characterization_and_expression_of_two_5-HTRs_from_the_pearl_oyster_Pinctada_fucata_martensii_xlsx/21193672 unknown doi:10.3389/fmars.2022.1011420.s002 https://figshare.com/articles/dataset/Table_2_Molecular_cloning_characterization_and_expression_of_two_5-HTRs_from_the_pearl_oyster_Pinctada_fucata_martensii_xlsx/21193672 CC BY 4.0 CC-BY Oceanography Marine Biology Marine Geoscience Biological Oceanography Chemical Oceanography Physical Oceanography Marine Engineering Pinctada fucata martensii Pm5-HTR2 Pm5-HTR4 larval metamorphosis molecular cloning Dataset 2022 ftfrontimediafig https://doi.org/10.3389/fmars.2022.1011420.s002 2022-09-28T23:12:04Z The receptors of serotonin, also known as 5-hydroxytryptamine receptor (5-HTR) can mediate regulatory metamorphosis processes in a variety of mollusks. Studying the mechanisms of metamorphosis of the pearl oyster is significant to elucidate breeding, resource recovery and marine pearl production. In this study, two 5-HTR genes from Pinctada fucata martensii (Pm5-HTR2 and Pm5-HTR4) were cloned. A total of 1623 bp open reading frame was identified in Pm5-HTR2, and a 1185 bp open reading frame was detected in Pm5-HTR4; these open reading frames encoded a 540-residue polypeptide and a 394-residue polypeptide, respectively. We also conducted a domain analysis, which indicated that Pm5-HTR2 and Pm5-HTR4 contained a seven-transmembrane domain and revealed that the receptors had high similarity to Crassostrea gigas 5-HTR2 (54.62%) and 5-HTR4 (66.23%). Sequence analysis demonstrated conserved advanced structure and motifs (the DRY/ERY and NPXXY motifs). The expression pattern analysis revealed high expression levels of Pm5-HTR2 and Pm5-HTR4 during the developmental stages. ISH analysis showed that Pm5-HTR2 was primarily expressed in the FE, B, T, EU, and EL stages and Pm5-HTR4 was mainly expressed in the FE, B, T, D, EU, and EL stages. These results suggest that 5-HTRs may play key roles in P. f. martensii larval metamorphosis. Dataset Crassostrea gigas Frontiers: Figshare |
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Frontiers: Figshare |
op_collection_id |
ftfrontimediafig |
language |
unknown |
topic |
Oceanography Marine Biology Marine Geoscience Biological Oceanography Chemical Oceanography Physical Oceanography Marine Engineering Pinctada fucata martensii Pm5-HTR2 Pm5-HTR4 larval metamorphosis molecular cloning |
spellingShingle |
Oceanography Marine Biology Marine Geoscience Biological Oceanography Chemical Oceanography Physical Oceanography Marine Engineering Pinctada fucata martensii Pm5-HTR2 Pm5-HTR4 larval metamorphosis molecular cloning Shaojie Zhu Yubo He Qiongyu Xu Jiabin Zhang Chuangye Yang Ruijuan Hao Junhui Li Yuewen Deng Table_2_Molecular cloning, characterization, and expression of two 5-HTRs from the pearl oyster Pinctada fucata martensii.xlsx |
topic_facet |
Oceanography Marine Biology Marine Geoscience Biological Oceanography Chemical Oceanography Physical Oceanography Marine Engineering Pinctada fucata martensii Pm5-HTR2 Pm5-HTR4 larval metamorphosis molecular cloning |
description |
The receptors of serotonin, also known as 5-hydroxytryptamine receptor (5-HTR) can mediate regulatory metamorphosis processes in a variety of mollusks. Studying the mechanisms of metamorphosis of the pearl oyster is significant to elucidate breeding, resource recovery and marine pearl production. In this study, two 5-HTR genes from Pinctada fucata martensii (Pm5-HTR2 and Pm5-HTR4) were cloned. A total of 1623 bp open reading frame was identified in Pm5-HTR2, and a 1185 bp open reading frame was detected in Pm5-HTR4; these open reading frames encoded a 540-residue polypeptide and a 394-residue polypeptide, respectively. We also conducted a domain analysis, which indicated that Pm5-HTR2 and Pm5-HTR4 contained a seven-transmembrane domain and revealed that the receptors had high similarity to Crassostrea gigas 5-HTR2 (54.62%) and 5-HTR4 (66.23%). Sequence analysis demonstrated conserved advanced structure and motifs (the DRY/ERY and NPXXY motifs). The expression pattern analysis revealed high expression levels of Pm5-HTR2 and Pm5-HTR4 during the developmental stages. ISH analysis showed that Pm5-HTR2 was primarily expressed in the FE, B, T, EU, and EL stages and Pm5-HTR4 was mainly expressed in the FE, B, T, D, EU, and EL stages. These results suggest that 5-HTRs may play key roles in P. f. martensii larval metamorphosis. |
format |
Dataset |
author |
Shaojie Zhu Yubo He Qiongyu Xu Jiabin Zhang Chuangye Yang Ruijuan Hao Junhui Li Yuewen Deng |
author_facet |
Shaojie Zhu Yubo He Qiongyu Xu Jiabin Zhang Chuangye Yang Ruijuan Hao Junhui Li Yuewen Deng |
author_sort |
Shaojie Zhu |
title |
Table_2_Molecular cloning, characterization, and expression of two 5-HTRs from the pearl oyster Pinctada fucata martensii.xlsx |
title_short |
Table_2_Molecular cloning, characterization, and expression of two 5-HTRs from the pearl oyster Pinctada fucata martensii.xlsx |
title_full |
Table_2_Molecular cloning, characterization, and expression of two 5-HTRs from the pearl oyster Pinctada fucata martensii.xlsx |
title_fullStr |
Table_2_Molecular cloning, characterization, and expression of two 5-HTRs from the pearl oyster Pinctada fucata martensii.xlsx |
title_full_unstemmed |
Table_2_Molecular cloning, characterization, and expression of two 5-HTRs from the pearl oyster Pinctada fucata martensii.xlsx |
title_sort |
table_2_molecular cloning, characterization, and expression of two 5-htrs from the pearl oyster pinctada fucata martensii.xlsx |
publishDate |
2022 |
url |
https://doi.org/10.3389/fmars.2022.1011420.s002 https://figshare.com/articles/dataset/Table_2_Molecular_cloning_characterization_and_expression_of_two_5-HTRs_from_the_pearl_oyster_Pinctada_fucata_martensii_xlsx/21193672 |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_relation |
doi:10.3389/fmars.2022.1011420.s002 https://figshare.com/articles/dataset/Table_2_Molecular_cloning_characterization_and_expression_of_two_5-HTRs_from_the_pearl_oyster_Pinctada_fucata_martensii_xlsx/21193672 |
op_rights |
CC BY 4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fmars.2022.1011420.s002 |
_version_ |
1766394616845697024 |