DataSheet1_Expression patterns of the poplar NF-Y gene family in response to Alternaria alternata and hormone treatment and the role of PdbNF-YA11 in disease resistance.docx
Plant nuclear factor-Y (NF-Y) transcription factors (TFs) are key regulators of growth and stress resistance. However, the role of NF-Y TFs in poplar in response to biotic stress is still unclear. In this study, we cloned 26 PdbNF-Y encoding genes in the hybrid poplar P. davidiana × P. bollena, incl...
Main Authors: | , , , , , , |
---|---|
Format: | Dataset |
Language: | unknown |
Published: |
2022
|
Subjects: | |
Online Access: | https://doi.org/10.3389/fbioe.2022.956271.s001 https://figshare.com/articles/dataset/DataSheet1_Expression_patterns_of_the_poplar_NF-Y_gene_family_in_response_to_Alternaria_alternata_and_hormone_treatment_and_the_role_of_PdbNF-YA11_in_disease_resistance_docx/21128329 |
id |
ftfrontimediafig:oai:figshare.com:article/21128329 |
---|---|
record_format |
openpolar |
spelling |
ftfrontimediafig:oai:figshare.com:article/21128329 2023-05-15T17:54:03+02:00 DataSheet1_Expression patterns of the poplar NF-Y gene family in response to Alternaria alternata and hormone treatment and the role of PdbNF-YA11 in disease resistance.docx Ying Huang Huijun Ma Xiaodong Wang Tianxiang Cui Gang Han Yu Zhang Chao Wang 2022-09-16T04:13:28Z https://doi.org/10.3389/fbioe.2022.956271.s001 https://figshare.com/articles/dataset/DataSheet1_Expression_patterns_of_the_poplar_NF-Y_gene_family_in_response_to_Alternaria_alternata_and_hormone_treatment_and_the_role_of_PdbNF-YA11_in_disease_resistance_docx/21128329 unknown doi:10.3389/fbioe.2022.956271.s001 https://figshare.com/articles/dataset/DataSheet1_Expression_patterns_of_the_poplar_NF-Y_gene_family_in_response_to_Alternaria_alternata_and_hormone_treatment_and_the_role_of_PdbNF-YA11_in_disease_resistance_docx/21128329 CC BY 4.0 CC-BY Biotechnology Biological Engineering Genetic Engineering Biomarkers Biomaterials Biomechanical Engineering Biomedical Engineering not elsewhere classified Synthetic Biology Agricultural Marine Biotechnology Bioremediation Bioprocessing Bioproduction and Bioproducts Industrial Biotechnology Diagnostics (incl. Biosensors) Industrial Microbiology (incl. Biofeedstocks) Industrial Molecular Engineering of Nucleic Acids and Proteins Industrial Biotechnology not elsewhere classified Medical Biotechnology Diagnostics (incl. Biosensors) Medical Molecular Engineering of Nucleic Acids and Proteins Regenerative Medicine (incl. Stem Cells and Tissue Engineering) Medical Biotechnology not elsewhere classified poplar NF-Y transcription factor expression pattern JA signal disease resistance Dataset 2022 ftfrontimediafig https://doi.org/10.3389/fbioe.2022.956271.s001 2022-09-21T23:10:11Z Plant nuclear factor-Y (NF-Y) transcription factors (TFs) are key regulators of growth and stress resistance. However, the role of NF-Y TFs in poplar in response to biotic stress is still unclear. In this study, we cloned 26 PdbNF-Y encoding genes in the hybrid poplar P. davidiana × P. bollena, including 12 PdbNF-YAs, six PdbNF-YBs, and eight PdbNF-YCs. Their physical and chemical parameters, conserved domains, and phylogeny were subsequently analyzed. The protein–protein interaction (PPI) network showed that the three PdbNF-Y subunits may interact with NF-Y proteins belonging to two other subfamilies and other TFs. Tissue expression analysis revealed that PdbNF-Ys exhibited three distinct expression patterns in three tissues. Cis-elements related to stress-responsiveness were found in the promoters of PdbNF-Ys, and most PdbNF-Ys were shown to be differentially expressed under Alternaria alternata and hormone treatments. Compared with the PdbNF-YB and PdbNF-YC subfamilies, more PdbNF-YAs were significantly induced under the two treatments. Moreover, loss- and gain-of-function analyses showed that PdbNF-YA11 plays a positive role in poplar resistance to A. alternata. Additionally, RT‒qPCR analyses showed that overexpression and silencing PdbNF-YA11 altered the transcript levels of JA-related genes, including LOX, AOS, AOC, COI, JAZ, ORCA, and MYC, suggesting that PdbNF-YA11-mediated disease resistance is related to activation of the JA pathway. Our findings will contribute to functional analysis of NF-Y genes in woody plants, especially their roles in response to biotic stress. Dataset Orca Frontiers: Figshare |
institution |
Open Polar |
collection |
Frontiers: Figshare |
op_collection_id |
ftfrontimediafig |
language |
unknown |
topic |
Biotechnology Biological Engineering Genetic Engineering Biomarkers Biomaterials Biomechanical Engineering Biomedical Engineering not elsewhere classified Synthetic Biology Agricultural Marine Biotechnology Bioremediation Bioprocessing Bioproduction and Bioproducts Industrial Biotechnology Diagnostics (incl. Biosensors) Industrial Microbiology (incl. Biofeedstocks) Industrial Molecular Engineering of Nucleic Acids and Proteins Industrial Biotechnology not elsewhere classified Medical Biotechnology Diagnostics (incl. Biosensors) Medical Molecular Engineering of Nucleic Acids and Proteins Regenerative Medicine (incl. Stem Cells and Tissue Engineering) Medical Biotechnology not elsewhere classified poplar NF-Y transcription factor expression pattern JA signal disease resistance |
spellingShingle |
Biotechnology Biological Engineering Genetic Engineering Biomarkers Biomaterials Biomechanical Engineering Biomedical Engineering not elsewhere classified Synthetic Biology Agricultural Marine Biotechnology Bioremediation Bioprocessing Bioproduction and Bioproducts Industrial Biotechnology Diagnostics (incl. Biosensors) Industrial Microbiology (incl. Biofeedstocks) Industrial Molecular Engineering of Nucleic Acids and Proteins Industrial Biotechnology not elsewhere classified Medical Biotechnology Diagnostics (incl. Biosensors) Medical Molecular Engineering of Nucleic Acids and Proteins Regenerative Medicine (incl. Stem Cells and Tissue Engineering) Medical Biotechnology not elsewhere classified poplar NF-Y transcription factor expression pattern JA signal disease resistance Ying Huang Huijun Ma Xiaodong Wang Tianxiang Cui Gang Han Yu Zhang Chao Wang DataSheet1_Expression patterns of the poplar NF-Y gene family in response to Alternaria alternata and hormone treatment and the role of PdbNF-YA11 in disease resistance.docx |
topic_facet |
Biotechnology Biological Engineering Genetic Engineering Biomarkers Biomaterials Biomechanical Engineering Biomedical Engineering not elsewhere classified Synthetic Biology Agricultural Marine Biotechnology Bioremediation Bioprocessing Bioproduction and Bioproducts Industrial Biotechnology Diagnostics (incl. Biosensors) Industrial Microbiology (incl. Biofeedstocks) Industrial Molecular Engineering of Nucleic Acids and Proteins Industrial Biotechnology not elsewhere classified Medical Biotechnology Diagnostics (incl. Biosensors) Medical Molecular Engineering of Nucleic Acids and Proteins Regenerative Medicine (incl. Stem Cells and Tissue Engineering) Medical Biotechnology not elsewhere classified poplar NF-Y transcription factor expression pattern JA signal disease resistance |
description |
Plant nuclear factor-Y (NF-Y) transcription factors (TFs) are key regulators of growth and stress resistance. However, the role of NF-Y TFs in poplar in response to biotic stress is still unclear. In this study, we cloned 26 PdbNF-Y encoding genes in the hybrid poplar P. davidiana × P. bollena, including 12 PdbNF-YAs, six PdbNF-YBs, and eight PdbNF-YCs. Their physical and chemical parameters, conserved domains, and phylogeny were subsequently analyzed. The protein–protein interaction (PPI) network showed that the three PdbNF-Y subunits may interact with NF-Y proteins belonging to two other subfamilies and other TFs. Tissue expression analysis revealed that PdbNF-Ys exhibited three distinct expression patterns in three tissues. Cis-elements related to stress-responsiveness were found in the promoters of PdbNF-Ys, and most PdbNF-Ys were shown to be differentially expressed under Alternaria alternata and hormone treatments. Compared with the PdbNF-YB and PdbNF-YC subfamilies, more PdbNF-YAs were significantly induced under the two treatments. Moreover, loss- and gain-of-function analyses showed that PdbNF-YA11 plays a positive role in poplar resistance to A. alternata. Additionally, RT‒qPCR analyses showed that overexpression and silencing PdbNF-YA11 altered the transcript levels of JA-related genes, including LOX, AOS, AOC, COI, JAZ, ORCA, and MYC, suggesting that PdbNF-YA11-mediated disease resistance is related to activation of the JA pathway. Our findings will contribute to functional analysis of NF-Y genes in woody plants, especially their roles in response to biotic stress. |
format |
Dataset |
author |
Ying Huang Huijun Ma Xiaodong Wang Tianxiang Cui Gang Han Yu Zhang Chao Wang |
author_facet |
Ying Huang Huijun Ma Xiaodong Wang Tianxiang Cui Gang Han Yu Zhang Chao Wang |
author_sort |
Ying Huang |
title |
DataSheet1_Expression patterns of the poplar NF-Y gene family in response to Alternaria alternata and hormone treatment and the role of PdbNF-YA11 in disease resistance.docx |
title_short |
DataSheet1_Expression patterns of the poplar NF-Y gene family in response to Alternaria alternata and hormone treatment and the role of PdbNF-YA11 in disease resistance.docx |
title_full |
DataSheet1_Expression patterns of the poplar NF-Y gene family in response to Alternaria alternata and hormone treatment and the role of PdbNF-YA11 in disease resistance.docx |
title_fullStr |
DataSheet1_Expression patterns of the poplar NF-Y gene family in response to Alternaria alternata and hormone treatment and the role of PdbNF-YA11 in disease resistance.docx |
title_full_unstemmed |
DataSheet1_Expression patterns of the poplar NF-Y gene family in response to Alternaria alternata and hormone treatment and the role of PdbNF-YA11 in disease resistance.docx |
title_sort |
datasheet1_expression patterns of the poplar nf-y gene family in response to alternaria alternata and hormone treatment and the role of pdbnf-ya11 in disease resistance.docx |
publishDate |
2022 |
url |
https://doi.org/10.3389/fbioe.2022.956271.s001 https://figshare.com/articles/dataset/DataSheet1_Expression_patterns_of_the_poplar_NF-Y_gene_family_in_response_to_Alternaria_alternata_and_hormone_treatment_and_the_role_of_PdbNF-YA11_in_disease_resistance_docx/21128329 |
genre |
Orca |
genre_facet |
Orca |
op_relation |
doi:10.3389/fbioe.2022.956271.s001 https://figshare.com/articles/dataset/DataSheet1_Expression_patterns_of_the_poplar_NF-Y_gene_family_in_response_to_Alternaria_alternata_and_hormone_treatment_and_the_role_of_PdbNF-YA11_in_disease_resistance_docx/21128329 |
op_rights |
CC BY 4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fbioe.2022.956271.s001 |
_version_ |
1766161761848786944 |