Table_5_Metabolic profiling and gene expression analyses provide insights into cold adaptation of an Antarctic moss Pohlia nutans.XLSX

Antarctica is the coldest, driest, and most windy continent on earth. The major terrestrial vegetation consists of cryptogams (mosses and lichens) and two vascular plant species. However, the molecular mechanism of cold tolerance and relevant regulatory networks were largely unknown in these Antarct...

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Main Authors: Shenghao Liu, Tingting Li, Shuo Fang, Pengying Zhang, Dan Yi, Bailin Cong, Zhaohui Zhang, Linlin Zhao
Format: Dataset
Language:unknown
Published: 2022
Subjects:
Online Access:https://doi.org/10.3389/fpls.2022.1006991.s008
https://figshare.com/articles/dataset/Table_5_Metabolic_profiling_and_gene_expression_analyses_provide_insights_into_cold_adaptation_of_an_Antarctic_moss_Pohlia_nutans_XLSX/21090199
id ftfrontimediafig:oai:figshare.com:article/21090199
record_format openpolar
spelling ftfrontimediafig:oai:figshare.com:article/21090199 2024-09-15T17:43:12+00:00 Table_5_Metabolic profiling and gene expression analyses provide insights into cold adaptation of an Antarctic moss Pohlia nutans.XLSX Shenghao Liu Tingting Li Shuo Fang Pengying Zhang Dan Yi Bailin Cong Zhaohui Zhang Linlin Zhao 2022-09-13T13:00:27Z https://doi.org/10.3389/fpls.2022.1006991.s008 https://figshare.com/articles/dataset/Table_5_Metabolic_profiling_and_gene_expression_analyses_provide_insights_into_cold_adaptation_of_an_Antarctic_moss_Pohlia_nutans_XLSX/21090199 unknown doi:10.3389/fpls.2022.1006991.s008 https://figshare.com/articles/dataset/Table_5_Metabolic_profiling_and_gene_expression_analyses_provide_insights_into_cold_adaptation_of_an_Antarctic_moss_Pohlia_nutans_XLSX/21090199 CC BY 4.0 Botany Plant Biology Plant Systematics and Taxonomy Plant Cell and Molecular Biology Plant Developmental and Reproductive Biology Plant Pathology Plant Physiology Plant Biology not elsewhere classified cold stress flavonoids very-long-chain fatty acids metabolomic profiling transcriptomic sequencing bryophytes Dataset 2022 ftfrontimediafig https://doi.org/10.3389/fpls.2022.1006991.s008 2024-08-19T06:20:02Z Antarctica is the coldest, driest, and most windy continent on earth. The major terrestrial vegetation consists of cryptogams (mosses and lichens) and two vascular plant species. However, the molecular mechanism of cold tolerance and relevant regulatory networks were largely unknown in these Antarctic plants. Here, we investigated the global alterations in metabolites and regulatory pathways of an Antarctic moss (Pohlia nutans) under cold stress using an integrated multi-omics approach. We found that proline content and several antioxidant enzyme activities were significantly increased in P. nutans under cold stress, but the contents of chlorophyll and total flavonoids were markedly decreased. A total of 559 metabolites were detected using ultra high-performance liquid chromatography/electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS). We observed 39 and 71 differentially changed metabolites (DCMs) after 24 h and 60 h cold stress, indicating that several major pathways were differentially activated for producing fatty acids, alkaloids, flavonoids, terpenoids, and phenolic acids. In addition, the quantitative transcriptome sequencing was conducted to uncover the global transcriptional profiles of P. nutans under cold stress. The representative differentially expressed genes (DEGs) were identified and summarized to the function including Ca 2+ signaling, ABA signaling, jasmonate signaling, fatty acids biosynthesis, flavonoid biosynthesis, and other biological processes. The integrated dataset analyses of metabolome and transcriptome revealed that jasmonate signaling, auxin signaling, very-long-chain fatty acids and flavonoid biosynthesis pathways might contribute to P. nutans acclimating to cold stress. Overall, these observations provide insight into Antarctic moss adaptations to polar habitats and the impact of global climate change on Antarctic plants. Dataset Antarc* Antarctic Antarctica Frontiers: Figshare
institution Open Polar
collection Frontiers: Figshare
op_collection_id ftfrontimediafig
language unknown
topic Botany
Plant Biology
Plant Systematics and Taxonomy
Plant Cell and Molecular Biology
Plant Developmental and Reproductive Biology
Plant Pathology
Plant Physiology
Plant Biology not elsewhere classified
cold stress
flavonoids
very-long-chain fatty acids
metabolomic profiling
transcriptomic sequencing
bryophytes
spellingShingle Botany
Plant Biology
Plant Systematics and Taxonomy
Plant Cell and Molecular Biology
Plant Developmental and Reproductive Biology
Plant Pathology
Plant Physiology
Plant Biology not elsewhere classified
cold stress
flavonoids
very-long-chain fatty acids
metabolomic profiling
transcriptomic sequencing
bryophytes
Shenghao Liu
Tingting Li
Shuo Fang
Pengying Zhang
Dan Yi
Bailin Cong
Zhaohui Zhang
Linlin Zhao
Table_5_Metabolic profiling and gene expression analyses provide insights into cold adaptation of an Antarctic moss Pohlia nutans.XLSX
topic_facet Botany
Plant Biology
Plant Systematics and Taxonomy
Plant Cell and Molecular Biology
Plant Developmental and Reproductive Biology
Plant Pathology
Plant Physiology
Plant Biology not elsewhere classified
cold stress
flavonoids
very-long-chain fatty acids
metabolomic profiling
transcriptomic sequencing
bryophytes
description Antarctica is the coldest, driest, and most windy continent on earth. The major terrestrial vegetation consists of cryptogams (mosses and lichens) and two vascular plant species. However, the molecular mechanism of cold tolerance and relevant regulatory networks were largely unknown in these Antarctic plants. Here, we investigated the global alterations in metabolites and regulatory pathways of an Antarctic moss (Pohlia nutans) under cold stress using an integrated multi-omics approach. We found that proline content and several antioxidant enzyme activities were significantly increased in P. nutans under cold stress, but the contents of chlorophyll and total flavonoids were markedly decreased. A total of 559 metabolites were detected using ultra high-performance liquid chromatography/electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS). We observed 39 and 71 differentially changed metabolites (DCMs) after 24 h and 60 h cold stress, indicating that several major pathways were differentially activated for producing fatty acids, alkaloids, flavonoids, terpenoids, and phenolic acids. In addition, the quantitative transcriptome sequencing was conducted to uncover the global transcriptional profiles of P. nutans under cold stress. The representative differentially expressed genes (DEGs) were identified and summarized to the function including Ca 2+ signaling, ABA signaling, jasmonate signaling, fatty acids biosynthesis, flavonoid biosynthesis, and other biological processes. The integrated dataset analyses of metabolome and transcriptome revealed that jasmonate signaling, auxin signaling, very-long-chain fatty acids and flavonoid biosynthesis pathways might contribute to P. nutans acclimating to cold stress. Overall, these observations provide insight into Antarctic moss adaptations to polar habitats and the impact of global climate change on Antarctic plants.
format Dataset
author Shenghao Liu
Tingting Li
Shuo Fang
Pengying Zhang
Dan Yi
Bailin Cong
Zhaohui Zhang
Linlin Zhao
author_facet Shenghao Liu
Tingting Li
Shuo Fang
Pengying Zhang
Dan Yi
Bailin Cong
Zhaohui Zhang
Linlin Zhao
author_sort Shenghao Liu
title Table_5_Metabolic profiling and gene expression analyses provide insights into cold adaptation of an Antarctic moss Pohlia nutans.XLSX
title_short Table_5_Metabolic profiling and gene expression analyses provide insights into cold adaptation of an Antarctic moss Pohlia nutans.XLSX
title_full Table_5_Metabolic profiling and gene expression analyses provide insights into cold adaptation of an Antarctic moss Pohlia nutans.XLSX
title_fullStr Table_5_Metabolic profiling and gene expression analyses provide insights into cold adaptation of an Antarctic moss Pohlia nutans.XLSX
title_full_unstemmed Table_5_Metabolic profiling and gene expression analyses provide insights into cold adaptation of an Antarctic moss Pohlia nutans.XLSX
title_sort table_5_metabolic profiling and gene expression analyses provide insights into cold adaptation of an antarctic moss pohlia nutans.xlsx
publishDate 2022
url https://doi.org/10.3389/fpls.2022.1006991.s008
https://figshare.com/articles/dataset/Table_5_Metabolic_profiling_and_gene_expression_analyses_provide_insights_into_cold_adaptation_of_an_Antarctic_moss_Pohlia_nutans_XLSX/21090199
genre Antarc*
Antarctic
Antarctica
genre_facet Antarc*
Antarctic
Antarctica
op_relation doi:10.3389/fpls.2022.1006991.s008
https://figshare.com/articles/dataset/Table_5_Metabolic_profiling_and_gene_expression_analyses_provide_insights_into_cold_adaptation_of_an_Antarctic_moss_Pohlia_nutans_XLSX/21090199
op_rights CC BY 4.0
op_doi https://doi.org/10.3389/fpls.2022.1006991.s008
_version_ 1810490063591571456