DataSheet_1_Vitamin D3 deficiency induced intestinal inflammatory response of turbot through nuclear factor-κB/inflammasome pathway, accompanied by the mutually exclusive apoptosis and autophagy.pdf
Vitamin D 3 (VD 3 ) participated widely in the nuclear factor-κB (NF-κB)-mediated inflammation, apoptosis, and autophagy through the vitamin D receptor (VDR). However, the molecular mechanisms remain not understood in teleost. The present study investigated the functions of VD 3 /VDR on intestinal i...
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Online Access: | https://doi.org/10.3389/fimmu.2022.986593.s001 https://figshare.com/articles/dataset/DataSheet_1_Vitamin_D3_deficiency_induced_intestinal_inflammatory_response_of_turbot_through_nuclear_factor-_B_inflammasome_pathway_accompanied_by_the_mutually_exclusive_apoptosis_and_autophagy_pdf/21061714 |
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ftfrontimediafig:oai:figshare.com:article/21061714 2024-09-15T18:39:59+00:00 DataSheet_1_Vitamin D3 deficiency induced intestinal inflammatory response of turbot through nuclear factor-κB/inflammasome pathway, accompanied by the mutually exclusive apoptosis and autophagy.pdf Zhichu Chen Dong Huang Prakaiwan Yongyut Guangbin Li María Ángeles Esteban Orapint Jintasataporn Junming Deng Wenbing Zhang Qinghui Ai Kangsen Mai Yanjiao Zhang 2022-09-08T06:04:05Z https://doi.org/10.3389/fimmu.2022.986593.s001 https://figshare.com/articles/dataset/DataSheet_1_Vitamin_D3_deficiency_induced_intestinal_inflammatory_response_of_turbot_through_nuclear_factor-_B_inflammasome_pathway_accompanied_by_the_mutually_exclusive_apoptosis_and_autophagy_pdf/21061714 unknown doi:10.3389/fimmu.2022.986593.s001 https://figshare.com/articles/dataset/DataSheet_1_Vitamin_D3_deficiency_induced_intestinal_inflammatory_response_of_turbot_through_nuclear_factor-_B_inflammasome_pathway_accompanied_by_the_mutually_exclusive_apoptosis_and_autophagy_pdf/21061714 CC BY 4.0 Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology vitamin D3 vitamin D3 receptor NF-κB inflammasome inflammation apoptosis autophagy Dataset 2022 ftfrontimediafig https://doi.org/10.3389/fimmu.2022.986593.s001 2024-08-19T06:19:49Z Vitamin D 3 (VD 3 ) participated widely in the nuclear factor-κB (NF-κB)-mediated inflammation, apoptosis, and autophagy through the vitamin D receptor (VDR). However, the molecular mechanisms remain not understood in teleost. The present study investigated the functions of VD 3 /VDR on intestinal inflammation, autophagy, and apoptosis of turbot in vivo and in vitro. Triple replicates of 30 fish were fed with each of three diets with graded levels of 32.0 (D 0 ), 1012.6 (D 1 ), and 3978.2 (D 2 ) IU/kg VD 3 . Obvious intestinal enteritis was observed in the D 0 group and followed with dysfunction of intestinal mucosal barriers. The intestinal inflammatory response induced by VD 3 deficiency was regulated by the NF-κB/inflammasome signalling. The promotion of intestinal apoptosis and suppression of intestinal autophagy were also observed in the D 0 group. Similarly, VD 3 deficiency in vitro induced more intense inflammation regulated by NF-κB/inflammasome signalling. The mutually exclusive apoptosis and autophagy were also observed in the group without 1,25(OH) 2 D 3 in vitro, accompanied by similar changes in apoptosis and autophagy increased apoptosis. The gene expression of VDRs was significantly increased with the increasing VD 3 supplementation both in vivo and in vitro. Moreover, VDR knockdown in turbot resulted in intestinal inflammation, and this process relied on the activation of inflammasome mediated by NF-κB signalling. Simultaneously, intestinal apoptosis was promoted, whereas intestinal autophagy was inhibited. In conclusion, VD 3 deficiency could induce intestinal inflammation via activation of the NF-κB/inflammasome pathway, intestinal apoptosis, and autophagy formed a mutually exclusive relation in teleost. And VDR is the critical molecule in those processes. Dataset Turbot Frontiers: Figshare |
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Frontiers: Figshare |
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ftfrontimediafig |
language |
unknown |
topic |
Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology vitamin D3 vitamin D3 receptor NF-κB inflammasome inflammation apoptosis autophagy |
spellingShingle |
Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology vitamin D3 vitamin D3 receptor NF-κB inflammasome inflammation apoptosis autophagy Zhichu Chen Dong Huang Prakaiwan Yongyut Guangbin Li María Ángeles Esteban Orapint Jintasataporn Junming Deng Wenbing Zhang Qinghui Ai Kangsen Mai Yanjiao Zhang DataSheet_1_Vitamin D3 deficiency induced intestinal inflammatory response of turbot through nuclear factor-κB/inflammasome pathway, accompanied by the mutually exclusive apoptosis and autophagy.pdf |
topic_facet |
Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology vitamin D3 vitamin D3 receptor NF-κB inflammasome inflammation apoptosis autophagy |
description |
Vitamin D 3 (VD 3 ) participated widely in the nuclear factor-κB (NF-κB)-mediated inflammation, apoptosis, and autophagy through the vitamin D receptor (VDR). However, the molecular mechanisms remain not understood in teleost. The present study investigated the functions of VD 3 /VDR on intestinal inflammation, autophagy, and apoptosis of turbot in vivo and in vitro. Triple replicates of 30 fish were fed with each of three diets with graded levels of 32.0 (D 0 ), 1012.6 (D 1 ), and 3978.2 (D 2 ) IU/kg VD 3 . Obvious intestinal enteritis was observed in the D 0 group and followed with dysfunction of intestinal mucosal barriers. The intestinal inflammatory response induced by VD 3 deficiency was regulated by the NF-κB/inflammasome signalling. The promotion of intestinal apoptosis and suppression of intestinal autophagy were also observed in the D 0 group. Similarly, VD 3 deficiency in vitro induced more intense inflammation regulated by NF-κB/inflammasome signalling. The mutually exclusive apoptosis and autophagy were also observed in the group without 1,25(OH) 2 D 3 in vitro, accompanied by similar changes in apoptosis and autophagy increased apoptosis. The gene expression of VDRs was significantly increased with the increasing VD 3 supplementation both in vivo and in vitro. Moreover, VDR knockdown in turbot resulted in intestinal inflammation, and this process relied on the activation of inflammasome mediated by NF-κB signalling. Simultaneously, intestinal apoptosis was promoted, whereas intestinal autophagy was inhibited. In conclusion, VD 3 deficiency could induce intestinal inflammation via activation of the NF-κB/inflammasome pathway, intestinal apoptosis, and autophagy formed a mutually exclusive relation in teleost. And VDR is the critical molecule in those processes. |
format |
Dataset |
author |
Zhichu Chen Dong Huang Prakaiwan Yongyut Guangbin Li María Ángeles Esteban Orapint Jintasataporn Junming Deng Wenbing Zhang Qinghui Ai Kangsen Mai Yanjiao Zhang |
author_facet |
Zhichu Chen Dong Huang Prakaiwan Yongyut Guangbin Li María Ángeles Esteban Orapint Jintasataporn Junming Deng Wenbing Zhang Qinghui Ai Kangsen Mai Yanjiao Zhang |
author_sort |
Zhichu Chen |
title |
DataSheet_1_Vitamin D3 deficiency induced intestinal inflammatory response of turbot through nuclear factor-κB/inflammasome pathway, accompanied by the mutually exclusive apoptosis and autophagy.pdf |
title_short |
DataSheet_1_Vitamin D3 deficiency induced intestinal inflammatory response of turbot through nuclear factor-κB/inflammasome pathway, accompanied by the mutually exclusive apoptosis and autophagy.pdf |
title_full |
DataSheet_1_Vitamin D3 deficiency induced intestinal inflammatory response of turbot through nuclear factor-κB/inflammasome pathway, accompanied by the mutually exclusive apoptosis and autophagy.pdf |
title_fullStr |
DataSheet_1_Vitamin D3 deficiency induced intestinal inflammatory response of turbot through nuclear factor-κB/inflammasome pathway, accompanied by the mutually exclusive apoptosis and autophagy.pdf |
title_full_unstemmed |
DataSheet_1_Vitamin D3 deficiency induced intestinal inflammatory response of turbot through nuclear factor-κB/inflammasome pathway, accompanied by the mutually exclusive apoptosis and autophagy.pdf |
title_sort |
datasheet_1_vitamin d3 deficiency induced intestinal inflammatory response of turbot through nuclear factor-κb/inflammasome pathway, accompanied by the mutually exclusive apoptosis and autophagy.pdf |
publishDate |
2022 |
url |
https://doi.org/10.3389/fimmu.2022.986593.s001 https://figshare.com/articles/dataset/DataSheet_1_Vitamin_D3_deficiency_induced_intestinal_inflammatory_response_of_turbot_through_nuclear_factor-_B_inflammasome_pathway_accompanied_by_the_mutually_exclusive_apoptosis_and_autophagy_pdf/21061714 |
genre |
Turbot |
genre_facet |
Turbot |
op_relation |
doi:10.3389/fimmu.2022.986593.s001 https://figshare.com/articles/dataset/DataSheet_1_Vitamin_D3_deficiency_induced_intestinal_inflammatory_response_of_turbot_through_nuclear_factor-_B_inflammasome_pathway_accompanied_by_the_mutually_exclusive_apoptosis_and_autophagy_pdf/21061714 |
op_rights |
CC BY 4.0 |
op_doi |
https://doi.org/10.3389/fimmu.2022.986593.s001 |
_version_ |
1810484325257314304 |