DataSheet_2_Single cell transcriptomics of Atlantic salmon (Salmo salar L.) liver reveals cellular heterogeneity and immunological responses to challenge by Aeromonas salmonicida.pdf
The liver is a multitasking organ with essential functions for vertebrate health spanning metabolism and immunity. In contrast to mammals, our understanding of liver cellular heterogeneity and its role in regulating immunological status remains poorly defined in fishes. Addressing this knowledge gap...
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Online Access: | https://doi.org/10.3389/fimmu.2022.984799.s002 https://figshare.com/articles/dataset/DataSheet_2_Single_cell_transcriptomics_of_Atlantic_salmon_Salmo_salar_L_liver_reveals_cellular_heterogeneity_and_immunological_responses_to_challenge_by_Aeromonas_salmonicida_pdf/20587086 |
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ftfrontimediafig:oai:figshare.com:article/20587086 2024-09-15T17:56:20+00:00 DataSheet_2_Single cell transcriptomics of Atlantic salmon (Salmo salar L.) liver reveals cellular heterogeneity and immunological responses to challenge by Aeromonas salmonicida.pdf Richard S. Taylor Rose Ruiz Daniels Ross Dobie Shahmir Naseer Thomas C. Clark Neil C. Henderson Pierre Boudinot Samuel A.M. Martin Daniel J. Macqueen 2022-08-24T10:58:05Z https://doi.org/10.3389/fimmu.2022.984799.s002 https://figshare.com/articles/dataset/DataSheet_2_Single_cell_transcriptomics_of_Atlantic_salmon_Salmo_salar_L_liver_reveals_cellular_heterogeneity_and_immunological_responses_to_challenge_by_Aeromonas_salmonicida_pdf/20587086 unknown doi:10.3389/fimmu.2022.984799.s002 https://figshare.com/articles/dataset/DataSheet_2_Single_cell_transcriptomics_of_Atlantic_salmon_Salmo_salar_L_liver_reveals_cellular_heterogeneity_and_immunological_responses_to_challenge_by_Aeromonas_salmonicida_pdf/20587086 CC BY 4.0 Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology liver single cell transcriptomics bacterial infection salmonid fish immune-metabolism cross talk Dataset 2022 ftfrontimediafig https://doi.org/10.3389/fimmu.2022.984799.s002 2024-08-19T06:20:02Z The liver is a multitasking organ with essential functions for vertebrate health spanning metabolism and immunity. In contrast to mammals, our understanding of liver cellular heterogeneity and its role in regulating immunological status remains poorly defined in fishes. Addressing this knowledge gap, we generated a transcriptomic atlas of 47,432 nuclei isolated from the liver of Atlantic salmon (Salmo salar L.) contrasting control fish with those challenged with a pathogenic strain of Aeromonas salmonicida, a problematic bacterial pathogen in global aquaculture. We identified the major liver cell types and their sub-populations, revealing poor conservation of many hepatic cell marker genes utilized in mammals, while identifying novel heterogeneity within the hepatocyte, lymphoid, and myeloid lineages. This included polyploid hepatocytes, multiple T cell populations including γδ T cells, and candidate populations of monocytes/macrophages and dendritic cells. A dominant hepatocyte population radically remodeled its transcriptome following infection to activate the acute phase response and other defense functions, while repressing routine functions such as metabolism. These defense-specialized hepatocytes showed strong activation of genes controlling protein synthesis and secretion, presumably to support the release of acute phase proteins into circulation. The infection response further involved up-regulation of numerous genes in an immune-cell specific manner, reflecting functions in pathogen recognition and killing, antigen presentation, phagocytosis, regulation of inflammation, B cell differentiation and T cell activation. Overall, this study greatly enhances our understanding of the multifaceted role played by liver immune and non-immune cells in host defense and metabolic remodeling following infection and provides many novel cell-specific marker genes to empower future studies of this organ in fishes. Dataset Atlantic salmon Salmo salar Frontiers: Figshare |
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Open Polar |
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Frontiers: Figshare |
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ftfrontimediafig |
language |
unknown |
topic |
Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology liver single cell transcriptomics bacterial infection salmonid fish immune-metabolism cross talk |
spellingShingle |
Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology liver single cell transcriptomics bacterial infection salmonid fish immune-metabolism cross talk Richard S. Taylor Rose Ruiz Daniels Ross Dobie Shahmir Naseer Thomas C. Clark Neil C. Henderson Pierre Boudinot Samuel A.M. Martin Daniel J. Macqueen DataSheet_2_Single cell transcriptomics of Atlantic salmon (Salmo salar L.) liver reveals cellular heterogeneity and immunological responses to challenge by Aeromonas salmonicida.pdf |
topic_facet |
Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology liver single cell transcriptomics bacterial infection salmonid fish immune-metabolism cross talk |
description |
The liver is a multitasking organ with essential functions for vertebrate health spanning metabolism and immunity. In contrast to mammals, our understanding of liver cellular heterogeneity and its role in regulating immunological status remains poorly defined in fishes. Addressing this knowledge gap, we generated a transcriptomic atlas of 47,432 nuclei isolated from the liver of Atlantic salmon (Salmo salar L.) contrasting control fish with those challenged with a pathogenic strain of Aeromonas salmonicida, a problematic bacterial pathogen in global aquaculture. We identified the major liver cell types and their sub-populations, revealing poor conservation of many hepatic cell marker genes utilized in mammals, while identifying novel heterogeneity within the hepatocyte, lymphoid, and myeloid lineages. This included polyploid hepatocytes, multiple T cell populations including γδ T cells, and candidate populations of monocytes/macrophages and dendritic cells. A dominant hepatocyte population radically remodeled its transcriptome following infection to activate the acute phase response and other defense functions, while repressing routine functions such as metabolism. These defense-specialized hepatocytes showed strong activation of genes controlling protein synthesis and secretion, presumably to support the release of acute phase proteins into circulation. The infection response further involved up-regulation of numerous genes in an immune-cell specific manner, reflecting functions in pathogen recognition and killing, antigen presentation, phagocytosis, regulation of inflammation, B cell differentiation and T cell activation. Overall, this study greatly enhances our understanding of the multifaceted role played by liver immune and non-immune cells in host defense and metabolic remodeling following infection and provides many novel cell-specific marker genes to empower future studies of this organ in fishes. |
format |
Dataset |
author |
Richard S. Taylor Rose Ruiz Daniels Ross Dobie Shahmir Naseer Thomas C. Clark Neil C. Henderson Pierre Boudinot Samuel A.M. Martin Daniel J. Macqueen |
author_facet |
Richard S. Taylor Rose Ruiz Daniels Ross Dobie Shahmir Naseer Thomas C. Clark Neil C. Henderson Pierre Boudinot Samuel A.M. Martin Daniel J. Macqueen |
author_sort |
Richard S. Taylor |
title |
DataSheet_2_Single cell transcriptomics of Atlantic salmon (Salmo salar L.) liver reveals cellular heterogeneity and immunological responses to challenge by Aeromonas salmonicida.pdf |
title_short |
DataSheet_2_Single cell transcriptomics of Atlantic salmon (Salmo salar L.) liver reveals cellular heterogeneity and immunological responses to challenge by Aeromonas salmonicida.pdf |
title_full |
DataSheet_2_Single cell transcriptomics of Atlantic salmon (Salmo salar L.) liver reveals cellular heterogeneity and immunological responses to challenge by Aeromonas salmonicida.pdf |
title_fullStr |
DataSheet_2_Single cell transcriptomics of Atlantic salmon (Salmo salar L.) liver reveals cellular heterogeneity and immunological responses to challenge by Aeromonas salmonicida.pdf |
title_full_unstemmed |
DataSheet_2_Single cell transcriptomics of Atlantic salmon (Salmo salar L.) liver reveals cellular heterogeneity and immunological responses to challenge by Aeromonas salmonicida.pdf |
title_sort |
datasheet_2_single cell transcriptomics of atlantic salmon (salmo salar l.) liver reveals cellular heterogeneity and immunological responses to challenge by aeromonas salmonicida.pdf |
publishDate |
2022 |
url |
https://doi.org/10.3389/fimmu.2022.984799.s002 https://figshare.com/articles/dataset/DataSheet_2_Single_cell_transcriptomics_of_Atlantic_salmon_Salmo_salar_L_liver_reveals_cellular_heterogeneity_and_immunological_responses_to_challenge_by_Aeromonas_salmonicida_pdf/20587086 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
doi:10.3389/fimmu.2022.984799.s002 https://figshare.com/articles/dataset/DataSheet_2_Single_cell_transcriptomics_of_Atlantic_salmon_Salmo_salar_L_liver_reveals_cellular_heterogeneity_and_immunological_responses_to_challenge_by_Aeromonas_salmonicida_pdf/20587086 |
op_rights |
CC BY 4.0 |
op_doi |
https://doi.org/10.3389/fimmu.2022.984799.s002 |
_version_ |
1810432547229794304 |