Table_1_Characterization and genomic analysis of two novel psychrotolerant Acidithiobacillus ferrooxidans strains from polar and subpolar environments.DOCX
The bioleaching process is carried out by aerobic acidophilic iron-oxidizing bacteria that are mainly mesophilic or moderately thermophilic. However, many mining sites are located in areas where the mean temperature is lower than the optimal growth temperature of these microorganisms. In this work,...
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ftfrontimediafig:oai:figshare.com:article/20578911 2024-09-15T17:43:37+00:00 Table_1_Characterization and genomic analysis of two novel psychrotolerant Acidithiobacillus ferrooxidans strains from polar and subpolar environments.DOCX Claudia Muñoz-Villagrán Jonnathan Grossolli-Gálvez Javiera Acevedo-Arbunic Ximena Valenzuela Alonso Ferrer Beatriz Díez Gloria Levicán 2022-08-24T05:01:52Z https://doi.org/10.3389/fmicb.2022.960324.s002 https://figshare.com/articles/dataset/Table_1_Characterization_and_genomic_analysis_of_two_novel_psychrotolerant_Acidithiobacillus_ferrooxidans_strains_from_polar_and_subpolar_environments_DOCX/20578911 unknown doi:10.3389/fmicb.2022.960324.s002 https://figshare.com/articles/dataset/Table_1_Characterization_and_genomic_analysis_of_two_novel_psychrotolerant_Acidithiobacillus_ferrooxidans_strains_from_polar_and_subpolar_environments_DOCX/20578911 CC BY 4.0 Microbiology Microbial Genetics Microbial Ecology Mycology Acidithiobacillus iron-oxidizing bacteria cold adaptations acidophiles Antarctic Chilean Patagonia Dataset 2022 ftfrontimediafig https://doi.org/10.3389/fmicb.2022.960324.s002 2024-08-19T06:20:02Z The bioleaching process is carried out by aerobic acidophilic iron-oxidizing bacteria that are mainly mesophilic or moderately thermophilic. However, many mining sites are located in areas where the mean temperature is lower than the optimal growth temperature of these microorganisms. In this work, we report the obtaining and characterization of two psychrotolerant bioleaching bacterial strains from low-temperature sites that included an abandoned mine site in Chilean Patagonia (PG05) and an acid rock drainage in Marian Cove, King George Island in Antarctic (MC2.2). The PG05 and MC2.2 strains showed significant iron-oxidation activity and grew optimally at 20°C. Genome sequence analyses showed chromosomes of 2.76 and 2.84 Mbp for PG05 and MC2.2, respectively, and an average nucleotide identity estimation indicated that both strains clustered with the acidophilic iron-oxidizing bacterium Acidithiobacillus ferrooxidans. The Patagonian PG05 strain had a high content of genes coding for tolerance to metals such as lead, zinc, and copper. Concordantly, electron microscopy revealed the intracellular presence of polyphosphate-like granules, likely involved in tolerance to metals and other stress conditions. The Antarctic MC2.2 strain showed a high dosage of genes for mercury resistance and low temperature adaptation. This report of cold-adapted cultures of the At. ferrooxidans species opens novel perspectives to satisfy the current challenges of the metal bioleaching industry. Dataset Antarc* Antarctic King George Island Frontiers: Figshare |
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Frontiers: Figshare |
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Microbiology Microbial Genetics Microbial Ecology Mycology Acidithiobacillus iron-oxidizing bacteria cold adaptations acidophiles Antarctic Chilean Patagonia |
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Microbiology Microbial Genetics Microbial Ecology Mycology Acidithiobacillus iron-oxidizing bacteria cold adaptations acidophiles Antarctic Chilean Patagonia Claudia Muñoz-Villagrán Jonnathan Grossolli-Gálvez Javiera Acevedo-Arbunic Ximena Valenzuela Alonso Ferrer Beatriz Díez Gloria Levicán Table_1_Characterization and genomic analysis of two novel psychrotolerant Acidithiobacillus ferrooxidans strains from polar and subpolar environments.DOCX |
topic_facet |
Microbiology Microbial Genetics Microbial Ecology Mycology Acidithiobacillus iron-oxidizing bacteria cold adaptations acidophiles Antarctic Chilean Patagonia |
description |
The bioleaching process is carried out by aerobic acidophilic iron-oxidizing bacteria that are mainly mesophilic or moderately thermophilic. However, many mining sites are located in areas where the mean temperature is lower than the optimal growth temperature of these microorganisms. In this work, we report the obtaining and characterization of two psychrotolerant bioleaching bacterial strains from low-temperature sites that included an abandoned mine site in Chilean Patagonia (PG05) and an acid rock drainage in Marian Cove, King George Island in Antarctic (MC2.2). The PG05 and MC2.2 strains showed significant iron-oxidation activity and grew optimally at 20°C. Genome sequence analyses showed chromosomes of 2.76 and 2.84 Mbp for PG05 and MC2.2, respectively, and an average nucleotide identity estimation indicated that both strains clustered with the acidophilic iron-oxidizing bacterium Acidithiobacillus ferrooxidans. The Patagonian PG05 strain had a high content of genes coding for tolerance to metals such as lead, zinc, and copper. Concordantly, electron microscopy revealed the intracellular presence of polyphosphate-like granules, likely involved in tolerance to metals and other stress conditions. The Antarctic MC2.2 strain showed a high dosage of genes for mercury resistance and low temperature adaptation. This report of cold-adapted cultures of the At. ferrooxidans species opens novel perspectives to satisfy the current challenges of the metal bioleaching industry. |
format |
Dataset |
author |
Claudia Muñoz-Villagrán Jonnathan Grossolli-Gálvez Javiera Acevedo-Arbunic Ximena Valenzuela Alonso Ferrer Beatriz Díez Gloria Levicán |
author_facet |
Claudia Muñoz-Villagrán Jonnathan Grossolli-Gálvez Javiera Acevedo-Arbunic Ximena Valenzuela Alonso Ferrer Beatriz Díez Gloria Levicán |
author_sort |
Claudia Muñoz-Villagrán |
title |
Table_1_Characterization and genomic analysis of two novel psychrotolerant Acidithiobacillus ferrooxidans strains from polar and subpolar environments.DOCX |
title_short |
Table_1_Characterization and genomic analysis of two novel psychrotolerant Acidithiobacillus ferrooxidans strains from polar and subpolar environments.DOCX |
title_full |
Table_1_Characterization and genomic analysis of two novel psychrotolerant Acidithiobacillus ferrooxidans strains from polar and subpolar environments.DOCX |
title_fullStr |
Table_1_Characterization and genomic analysis of two novel psychrotolerant Acidithiobacillus ferrooxidans strains from polar and subpolar environments.DOCX |
title_full_unstemmed |
Table_1_Characterization and genomic analysis of two novel psychrotolerant Acidithiobacillus ferrooxidans strains from polar and subpolar environments.DOCX |
title_sort |
table_1_characterization and genomic analysis of two novel psychrotolerant acidithiobacillus ferrooxidans strains from polar and subpolar environments.docx |
publishDate |
2022 |
url |
https://doi.org/10.3389/fmicb.2022.960324.s002 https://figshare.com/articles/dataset/Table_1_Characterization_and_genomic_analysis_of_two_novel_psychrotolerant_Acidithiobacillus_ferrooxidans_strains_from_polar_and_subpolar_environments_DOCX/20578911 |
genre |
Antarc* Antarctic King George Island |
genre_facet |
Antarc* Antarctic King George Island |
op_relation |
doi:10.3389/fmicb.2022.960324.s002 https://figshare.com/articles/dataset/Table_1_Characterization_and_genomic_analysis_of_two_novel_psychrotolerant_Acidithiobacillus_ferrooxidans_strains_from_polar_and_subpolar_environments_DOCX/20578911 |
op_rights |
CC BY 4.0 |
op_doi |
https://doi.org/10.3389/fmicb.2022.960324.s002 |
_version_ |
1810490655336562688 |