Image1_Elevated exposures to persistent endocrine disrupting compounds impact the sperm methylome in regions associated with autism spectrum disorder.TIF
Environmental exposures to endocrine disrupting compounds (EDCs) such as the organochlorines have been linked with various diseases including neurodevelopmental disorders. Autism spectrum disorder (ASD) is a highly complex neurodevelopmental disorder that is considered strongly genetic in origin due...
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ftfrontimediafig:oai:figshare.com:article/20471613 2024-09-15T18:05:36+00:00 Image1_Elevated exposures to persistent endocrine disrupting compounds impact the sperm methylome in regions associated with autism spectrum disorder.TIF Angela G. Maggio Henry T. Shu Benjamin I. Laufer Chongfeng Bi Yinglei Lai Janine M. LaSalle Valerie W. Hu 2022-08-11T05:42:32Z https://doi.org/10.3389/fgene.2022.929471.s002 https://figshare.com/articles/figure/Image1_Elevated_exposures_to_persistent_endocrine_disrupting_compounds_impact_the_sperm_methylome_in_regions_associated_with_autism_spectrum_disorder_TIF/20471613 unknown doi:10.3389/fgene.2022.929471.s002 https://figshare.com/articles/figure/Image1_Elevated_exposures_to_persistent_endocrine_disrupting_compounds_impact_the_sperm_methylome_in_regions_associated_with_autism_spectrum_disorder_TIF/20471613 CC BY 4.0 Genetics Genetic Engineering Biomarkers Developmental Genetics (incl. Sex Determination) Epigenetics (incl. Genome Methylation and Epigenomics) Gene Expression (incl. Microarray and other genome-wide approaches) Genome Structure and Regulation Genomics Genetically Modified Animals Livestock Cloning Gene and Molecular Therapy endocrine disrupting compounds DNA methylation sperm Faroe Islands autism Image Figure 2022 ftfrontimediafig https://doi.org/10.3389/fgene.2022.929471.s002 2024-08-19T06:19:48Z Environmental exposures to endocrine disrupting compounds (EDCs) such as the organochlorines have been linked with various diseases including neurodevelopmental disorders. Autism spectrum disorder (ASD) is a highly complex neurodevelopmental disorder that is considered strongly genetic in origin due to its high heritability. However, the rapidly rising prevalence of ASD suggests that environmental factors may also influence risk for ASD. In the present study, whole genome bisulfite sequencing was used to identify genome-wide differentially methylated regions (DMRs) in a total of 52 sperm samples from a cohort of men from the Faroe Islands (Denmark) who were equally divided into high and low exposure groups based on their serum levels of the long-lived organochlorine 1,1-dichloro-2,2-bis(p-chlorophenyl)ethylene (DDE), a primary breakdown product of the now banned insecticide dichlorodiphenyltrichloroethane (DDT). Aside from being considered a genetic isolate, inhabitants of the Faroe Islands have a native diet that potentially exposes them to a wide range of seafood neurotoxicants in the form of persistent organic pollutants (POPs). The DMRs were mapped to the human genome using Bismark, a 3-letter aligner used for methyl-seq analyses. Gene ontology, functional, and pathway analyses of the DMR-associated genes showed significant enrichment for genes involved in neurological functions and neurodevelopmental processes frequently impacted by ASD. Notably, these genes also significantly overlap with autism risk genes as well as those previously identified in sperm from fathers of children with ASD in comparison to that of fathers of neurotypical children. These results collectively suggest a possible mechanism involving altered methylation of a significant number of neurologically relevant ASD risk genes for introducing epigenetic changes associated with environmental exposures into the sperm methylome. Such changes may provide the potential for transgenerational inheritance of ASD as well as other disorders. Still Image Faroe Islands Frontiers: Figshare |
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Frontiers: Figshare |
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ftfrontimediafig |
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unknown |
topic |
Genetics Genetic Engineering Biomarkers Developmental Genetics (incl. Sex Determination) Epigenetics (incl. Genome Methylation and Epigenomics) Gene Expression (incl. Microarray and other genome-wide approaches) Genome Structure and Regulation Genomics Genetically Modified Animals Livestock Cloning Gene and Molecular Therapy endocrine disrupting compounds DNA methylation sperm Faroe Islands autism |
spellingShingle |
Genetics Genetic Engineering Biomarkers Developmental Genetics (incl. Sex Determination) Epigenetics (incl. Genome Methylation and Epigenomics) Gene Expression (incl. Microarray and other genome-wide approaches) Genome Structure and Regulation Genomics Genetically Modified Animals Livestock Cloning Gene and Molecular Therapy endocrine disrupting compounds DNA methylation sperm Faroe Islands autism Angela G. Maggio Henry T. Shu Benjamin I. Laufer Chongfeng Bi Yinglei Lai Janine M. LaSalle Valerie W. Hu Image1_Elevated exposures to persistent endocrine disrupting compounds impact the sperm methylome in regions associated with autism spectrum disorder.TIF |
topic_facet |
Genetics Genetic Engineering Biomarkers Developmental Genetics (incl. Sex Determination) Epigenetics (incl. Genome Methylation and Epigenomics) Gene Expression (incl. Microarray and other genome-wide approaches) Genome Structure and Regulation Genomics Genetically Modified Animals Livestock Cloning Gene and Molecular Therapy endocrine disrupting compounds DNA methylation sperm Faroe Islands autism |
description |
Environmental exposures to endocrine disrupting compounds (EDCs) such as the organochlorines have been linked with various diseases including neurodevelopmental disorders. Autism spectrum disorder (ASD) is a highly complex neurodevelopmental disorder that is considered strongly genetic in origin due to its high heritability. However, the rapidly rising prevalence of ASD suggests that environmental factors may also influence risk for ASD. In the present study, whole genome bisulfite sequencing was used to identify genome-wide differentially methylated regions (DMRs) in a total of 52 sperm samples from a cohort of men from the Faroe Islands (Denmark) who were equally divided into high and low exposure groups based on their serum levels of the long-lived organochlorine 1,1-dichloro-2,2-bis(p-chlorophenyl)ethylene (DDE), a primary breakdown product of the now banned insecticide dichlorodiphenyltrichloroethane (DDT). Aside from being considered a genetic isolate, inhabitants of the Faroe Islands have a native diet that potentially exposes them to a wide range of seafood neurotoxicants in the form of persistent organic pollutants (POPs). The DMRs were mapped to the human genome using Bismark, a 3-letter aligner used for methyl-seq analyses. Gene ontology, functional, and pathway analyses of the DMR-associated genes showed significant enrichment for genes involved in neurological functions and neurodevelopmental processes frequently impacted by ASD. Notably, these genes also significantly overlap with autism risk genes as well as those previously identified in sperm from fathers of children with ASD in comparison to that of fathers of neurotypical children. These results collectively suggest a possible mechanism involving altered methylation of a significant number of neurologically relevant ASD risk genes for introducing epigenetic changes associated with environmental exposures into the sperm methylome. Such changes may provide the potential for transgenerational inheritance of ASD as well as other disorders. |
format |
Still Image |
author |
Angela G. Maggio Henry T. Shu Benjamin I. Laufer Chongfeng Bi Yinglei Lai Janine M. LaSalle Valerie W. Hu |
author_facet |
Angela G. Maggio Henry T. Shu Benjamin I. Laufer Chongfeng Bi Yinglei Lai Janine M. LaSalle Valerie W. Hu |
author_sort |
Angela G. Maggio |
title |
Image1_Elevated exposures to persistent endocrine disrupting compounds impact the sperm methylome in regions associated with autism spectrum disorder.TIF |
title_short |
Image1_Elevated exposures to persistent endocrine disrupting compounds impact the sperm methylome in regions associated with autism spectrum disorder.TIF |
title_full |
Image1_Elevated exposures to persistent endocrine disrupting compounds impact the sperm methylome in regions associated with autism spectrum disorder.TIF |
title_fullStr |
Image1_Elevated exposures to persistent endocrine disrupting compounds impact the sperm methylome in regions associated with autism spectrum disorder.TIF |
title_full_unstemmed |
Image1_Elevated exposures to persistent endocrine disrupting compounds impact the sperm methylome in regions associated with autism spectrum disorder.TIF |
title_sort |
image1_elevated exposures to persistent endocrine disrupting compounds impact the sperm methylome in regions associated with autism spectrum disorder.tif |
publishDate |
2022 |
url |
https://doi.org/10.3389/fgene.2022.929471.s002 https://figshare.com/articles/figure/Image1_Elevated_exposures_to_persistent_endocrine_disrupting_compounds_impact_the_sperm_methylome_in_regions_associated_with_autism_spectrum_disorder_TIF/20471613 |
genre |
Faroe Islands |
genre_facet |
Faroe Islands |
op_relation |
doi:10.3389/fgene.2022.929471.s002 https://figshare.com/articles/figure/Image1_Elevated_exposures_to_persistent_endocrine_disrupting_compounds_impact_the_sperm_methylome_in_regions_associated_with_autism_spectrum_disorder_TIF/20471613 |
op_rights |
CC BY 4.0 |
op_doi |
https://doi.org/10.3389/fgene.2022.929471.s002 |
_version_ |
1810443138190278656 |