Table_2_Integrated transcriptome and metabolome analyses reveal the adaptation of Antarctic moss Pohlia nutans to drought stress.XLSX
Most regions of the Antarctic continent are experiencing increased dryness due to global climate change. Mosses and lichens are the dominant vegetation of the ice-free areas of Antarctica. However, the molecular mechanisms of these Antarctic plants adapting to drought stress are less documented. Her...
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ftfrontimediafig:oai:figshare.com:article/20471271 2023-05-15T13:55:09+02:00 Table_2_Integrated transcriptome and metabolome analyses reveal the adaptation of Antarctic moss Pohlia nutans to drought stress.XLSX Shuo Fang Tingting Li Pengying Zhang Chenlin Liu Bailin Cong Shenghao Liu 2022-08-11T05:25:02Z https://doi.org/10.3389/fpls.2022.924162.s002 https://figshare.com/articles/dataset/Table_2_Integrated_transcriptome_and_metabolome_analyses_reveal_the_adaptation_of_Antarctic_moss_Pohlia_nutans_to_drought_stress_XLSX/20471271 unknown doi:10.3389/fpls.2022.924162.s002 https://figshare.com/articles/dataset/Table_2_Integrated_transcriptome_and_metabolome_analyses_reveal_the_adaptation_of_Antarctic_moss_Pohlia_nutans_to_drought_stress_XLSX/20471271 CC BY 4.0 CC-BY Botany Plant Biology Plant Systematics and Taxonomy Plant Cell and Molecular Biology Plant Developmental and Reproductive Biology Plant Pathology Plant Physiology Plant Biology not elsewhere classified abiotic stress flavonoids metabolome transcriptome abscisic acid bryophytes Dataset 2022 ftfrontimediafig https://doi.org/10.3389/fpls.2022.924162.s002 2022-08-17T23:10:36Z Most regions of the Antarctic continent are experiencing increased dryness due to global climate change. Mosses and lichens are the dominant vegetation of the ice-free areas of Antarctica. However, the molecular mechanisms of these Antarctic plants adapting to drought stress are less documented. Here, transcriptome and metabolome analyses were employed to reveal the responses of an Antarctic moss (Pohlia nutans subsp. LIU) to drought stress. We found that drought stress made the gametophytes turn yellow and curled, and enhanced the contents of malondialdehyde and proline, and the activities of antioxidant enzymes. Totally, 2,451 differentially expressed genes (DEGs) were uncovered under drought treatment. The representative DEGs are mainly involved in ROS-scavenging and detoxification, flavonoid metabolism pathway, plant hormone signaling pathway, lipids metabolism pathway, transcription factors and signal-related genes. Meanwhile, a total of 354 differentially changed metabolites (DCMs) were detected in the metabolome analysis. Flavonoids and lipids were the most abundant metabolites and they accounted for 41.53% of the significantly changed metabolites. In addition, integrated transcriptome and metabolome analyses revealed co-expression patterns of flavonoid and long-chain fatty acid biosynthesis genes and their metabolites. Finally, qPCR analysis demonstrated that the expression levels of stress-related genes were significantly increased. These genes included those involved in ABA signaling pathway (NCED3, PP2C, PYL, and SnAK2), jasmonate signaling pathway (AOC, AOS, JAZ, and OPR), flavonoid pathway (CHS, F3’,5’H, F3H, FLS, FNS, and UFGT), antioxidant and detoxifying functions (POD, GSH-Px, Prx and DTX), and transcription factors (ERF and DREB). In summary, we speculated that P. nutans were highly dependent on ABA and jasmonate signaling pathways, ROS scavenging, flavonoids and fatty acid metabolism in response to drought stress. These findings present an important knowledge for assessing the impact of ... Dataset Antarc* Antarctic Antarctica Frontiers: Figshare Antarctic The Antarctic |
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Open Polar |
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Frontiers: Figshare |
op_collection_id |
ftfrontimediafig |
language |
unknown |
topic |
Botany Plant Biology Plant Systematics and Taxonomy Plant Cell and Molecular Biology Plant Developmental and Reproductive Biology Plant Pathology Plant Physiology Plant Biology not elsewhere classified abiotic stress flavonoids metabolome transcriptome abscisic acid bryophytes |
spellingShingle |
Botany Plant Biology Plant Systematics and Taxonomy Plant Cell and Molecular Biology Plant Developmental and Reproductive Biology Plant Pathology Plant Physiology Plant Biology not elsewhere classified abiotic stress flavonoids metabolome transcriptome abscisic acid bryophytes Shuo Fang Tingting Li Pengying Zhang Chenlin Liu Bailin Cong Shenghao Liu Table_2_Integrated transcriptome and metabolome analyses reveal the adaptation of Antarctic moss Pohlia nutans to drought stress.XLSX |
topic_facet |
Botany Plant Biology Plant Systematics and Taxonomy Plant Cell and Molecular Biology Plant Developmental and Reproductive Biology Plant Pathology Plant Physiology Plant Biology not elsewhere classified abiotic stress flavonoids metabolome transcriptome abscisic acid bryophytes |
description |
Most regions of the Antarctic continent are experiencing increased dryness due to global climate change. Mosses and lichens are the dominant vegetation of the ice-free areas of Antarctica. However, the molecular mechanisms of these Antarctic plants adapting to drought stress are less documented. Here, transcriptome and metabolome analyses were employed to reveal the responses of an Antarctic moss (Pohlia nutans subsp. LIU) to drought stress. We found that drought stress made the gametophytes turn yellow and curled, and enhanced the contents of malondialdehyde and proline, and the activities of antioxidant enzymes. Totally, 2,451 differentially expressed genes (DEGs) were uncovered under drought treatment. The representative DEGs are mainly involved in ROS-scavenging and detoxification, flavonoid metabolism pathway, plant hormone signaling pathway, lipids metabolism pathway, transcription factors and signal-related genes. Meanwhile, a total of 354 differentially changed metabolites (DCMs) were detected in the metabolome analysis. Flavonoids and lipids were the most abundant metabolites and they accounted for 41.53% of the significantly changed metabolites. In addition, integrated transcriptome and metabolome analyses revealed co-expression patterns of flavonoid and long-chain fatty acid biosynthesis genes and their metabolites. Finally, qPCR analysis demonstrated that the expression levels of stress-related genes were significantly increased. These genes included those involved in ABA signaling pathway (NCED3, PP2C, PYL, and SnAK2), jasmonate signaling pathway (AOC, AOS, JAZ, and OPR), flavonoid pathway (CHS, F3’,5’H, F3H, FLS, FNS, and UFGT), antioxidant and detoxifying functions (POD, GSH-Px, Prx and DTX), and transcription factors (ERF and DREB). In summary, we speculated that P. nutans were highly dependent on ABA and jasmonate signaling pathways, ROS scavenging, flavonoids and fatty acid metabolism in response to drought stress. These findings present an important knowledge for assessing the impact of ... |
format |
Dataset |
author |
Shuo Fang Tingting Li Pengying Zhang Chenlin Liu Bailin Cong Shenghao Liu |
author_facet |
Shuo Fang Tingting Li Pengying Zhang Chenlin Liu Bailin Cong Shenghao Liu |
author_sort |
Shuo Fang |
title |
Table_2_Integrated transcriptome and metabolome analyses reveal the adaptation of Antarctic moss Pohlia nutans to drought stress.XLSX |
title_short |
Table_2_Integrated transcriptome and metabolome analyses reveal the adaptation of Antarctic moss Pohlia nutans to drought stress.XLSX |
title_full |
Table_2_Integrated transcriptome and metabolome analyses reveal the adaptation of Antarctic moss Pohlia nutans to drought stress.XLSX |
title_fullStr |
Table_2_Integrated transcriptome and metabolome analyses reveal the adaptation of Antarctic moss Pohlia nutans to drought stress.XLSX |
title_full_unstemmed |
Table_2_Integrated transcriptome and metabolome analyses reveal the adaptation of Antarctic moss Pohlia nutans to drought stress.XLSX |
title_sort |
table_2_integrated transcriptome and metabolome analyses reveal the adaptation of antarctic moss pohlia nutans to drought stress.xlsx |
publishDate |
2022 |
url |
https://doi.org/10.3389/fpls.2022.924162.s002 https://figshare.com/articles/dataset/Table_2_Integrated_transcriptome_and_metabolome_analyses_reveal_the_adaptation_of_Antarctic_moss_Pohlia_nutans_to_drought_stress_XLSX/20471271 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic Antarctica |
genre_facet |
Antarc* Antarctic Antarctica |
op_relation |
doi:10.3389/fpls.2022.924162.s002 https://figshare.com/articles/dataset/Table_2_Integrated_transcriptome_and_metabolome_analyses_reveal_the_adaptation_of_Antarctic_moss_Pohlia_nutans_to_drought_stress_XLSX/20471271 |
op_rights |
CC BY 4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fpls.2022.924162.s002 |
_version_ |
1766261421361856512 |