Table_1_Effect of diet on molecular relationships between Atlantic cod larval muscle growth dynamics, metabolism, and antioxidant defense system.xlsx
We studied molecular effects (RNAseq and qPCR) of first feeding prey types (copepods or rotifers/Artemia) on skeletal muscle myogenesis and growth dynamics (proliferation, differentiation), metabolism (glycolysis, gluconeogenesis, oxidative phosphorylation), and antioxidant defense system (productio...
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ftfrontimediafig:oai:figshare.com:article/20462007 2024-09-09T19:29:47+00:00 Table_1_Effect of diet on molecular relationships between Atlantic cod larval muscle growth dynamics, metabolism, and antioxidant defense system.xlsx Tu A. Vo Trina F. Galloway Augustine Arukwe Rolf B. Edvardsen Kristin Hamre Ørjan Karlsen Ivar Rønnestad Elin Kjørsvik 2022-08-10T06:43:59Z https://doi.org/10.3389/fmars.2022.814022.s001 https://figshare.com/articles/dataset/Table_1_Effect_of_diet_on_molecular_relationships_between_Atlantic_cod_larval_muscle_growth_dynamics_metabolism_and_antioxidant_defense_system_xlsx/20462007 unknown doi:10.3389/fmars.2022.814022.s001 https://figshare.com/articles/dataset/Table_1_Effect_of_diet_on_molecular_relationships_between_Atlantic_cod_larval_muscle_growth_dynamics_metabolism_and_antioxidant_defense_system_xlsx/20462007 CC BY 4.0 Oceanography Marine Biology Marine Geoscience Biological Oceanography Chemical Oceanography Physical Oceanography Marine Engineering Atlantic cod larvae larval nutrition muscle hypertrophy and hyperplasia larval metabolism antioxidant defense system muscle growth and differentiation Gadus morhua (L.) larval growth Dataset 2022 ftfrontimediafig https://doi.org/10.3389/fmars.2022.814022.s001 2024-08-19T06:19:48Z We studied molecular effects (RNAseq and qPCR) of first feeding prey types (copepods or rotifers/Artemia) on skeletal muscle myogenesis and growth dynamics (proliferation, differentiation), metabolism (glycolysis, gluconeogenesis, oxidative phosphorylation), and antioxidant defense system (production/regulation of reactive oxygen species (ROS) in cod (Gadus morhua) larval skeletal muscle. Larval somatic growth rates were significantly higher in copepod fed larvae, although shifts in gene expressions related to muscle growth dynamics between hypertrophy and hyperplasia and generation and regulation of ROS mostly occurred around 5-, 10-, and 15-mm standard length (SL) for both groups. Gene expression for cell membrane proteins (such as nox1 and igf1r) peaked at 7 mm SL in all larvae, corresponding with increased ROS expressions in cod muscle during the exponential stratified hyperplasia phase from 7 mm SL. Expression for muscle differentiation (mef2a) occurred continuously (strongest from 10 mm SL). Expressions for muscle proliferation (pcna) and hydrogen peroxide (H 2 O 2 ) generation (sod1 and sod2) occurred in the 5 - 15 mm SL range, peaking at 10 mm SL in all larvae. A downregulation of sod1 and sod2 in skeletal muscle from 15 mm SL indicated the first response of the defense antioxidant system. Gene expressions related to glucose metabolism (slc2A11, pfk, fpb2, ldha) was 3 - 10 times higher in copepod-fed larvae than in rotifer/Artemia-fed larvae between 7 – 10 mm (live prey period). Copepods move faster than rotifers, and cod larvae will also gradually increase their active swimming periods, due to less viscous forces. Active swimming during the strongest muscle stratified hyperplasia phase (7 – 10 mm SL) could promote a better delivery and transport across the muscle membrane and intracellular flux through glycolysis and oxidative phosphorylation and would contribute to the observed earlier and more effective glucose metabolism in the larvae fed copepods. We suggest that active swimming is an important ... Dataset atlantic cod Gadus morhua Copepods Rotifer Frontiers: Figshare |
institution |
Open Polar |
collection |
Frontiers: Figshare |
op_collection_id |
ftfrontimediafig |
language |
unknown |
topic |
Oceanography Marine Biology Marine Geoscience Biological Oceanography Chemical Oceanography Physical Oceanography Marine Engineering Atlantic cod larvae larval nutrition muscle hypertrophy and hyperplasia larval metabolism antioxidant defense system muscle growth and differentiation Gadus morhua (L.) larval growth |
spellingShingle |
Oceanography Marine Biology Marine Geoscience Biological Oceanography Chemical Oceanography Physical Oceanography Marine Engineering Atlantic cod larvae larval nutrition muscle hypertrophy and hyperplasia larval metabolism antioxidant defense system muscle growth and differentiation Gadus morhua (L.) larval growth Tu A. Vo Trina F. Galloway Augustine Arukwe Rolf B. Edvardsen Kristin Hamre Ørjan Karlsen Ivar Rønnestad Elin Kjørsvik Table_1_Effect of diet on molecular relationships between Atlantic cod larval muscle growth dynamics, metabolism, and antioxidant defense system.xlsx |
topic_facet |
Oceanography Marine Biology Marine Geoscience Biological Oceanography Chemical Oceanography Physical Oceanography Marine Engineering Atlantic cod larvae larval nutrition muscle hypertrophy and hyperplasia larval metabolism antioxidant defense system muscle growth and differentiation Gadus morhua (L.) larval growth |
description |
We studied molecular effects (RNAseq and qPCR) of first feeding prey types (copepods or rotifers/Artemia) on skeletal muscle myogenesis and growth dynamics (proliferation, differentiation), metabolism (glycolysis, gluconeogenesis, oxidative phosphorylation), and antioxidant defense system (production/regulation of reactive oxygen species (ROS) in cod (Gadus morhua) larval skeletal muscle. Larval somatic growth rates were significantly higher in copepod fed larvae, although shifts in gene expressions related to muscle growth dynamics between hypertrophy and hyperplasia and generation and regulation of ROS mostly occurred around 5-, 10-, and 15-mm standard length (SL) for both groups. Gene expression for cell membrane proteins (such as nox1 and igf1r) peaked at 7 mm SL in all larvae, corresponding with increased ROS expressions in cod muscle during the exponential stratified hyperplasia phase from 7 mm SL. Expression for muscle differentiation (mef2a) occurred continuously (strongest from 10 mm SL). Expressions for muscle proliferation (pcna) and hydrogen peroxide (H 2 O 2 ) generation (sod1 and sod2) occurred in the 5 - 15 mm SL range, peaking at 10 mm SL in all larvae. A downregulation of sod1 and sod2 in skeletal muscle from 15 mm SL indicated the first response of the defense antioxidant system. Gene expressions related to glucose metabolism (slc2A11, pfk, fpb2, ldha) was 3 - 10 times higher in copepod-fed larvae than in rotifer/Artemia-fed larvae between 7 – 10 mm (live prey period). Copepods move faster than rotifers, and cod larvae will also gradually increase their active swimming periods, due to less viscous forces. Active swimming during the strongest muscle stratified hyperplasia phase (7 – 10 mm SL) could promote a better delivery and transport across the muscle membrane and intracellular flux through glycolysis and oxidative phosphorylation and would contribute to the observed earlier and more effective glucose metabolism in the larvae fed copepods. We suggest that active swimming is an important ... |
format |
Dataset |
author |
Tu A. Vo Trina F. Galloway Augustine Arukwe Rolf B. Edvardsen Kristin Hamre Ørjan Karlsen Ivar Rønnestad Elin Kjørsvik |
author_facet |
Tu A. Vo Trina F. Galloway Augustine Arukwe Rolf B. Edvardsen Kristin Hamre Ørjan Karlsen Ivar Rønnestad Elin Kjørsvik |
author_sort |
Tu A. Vo |
title |
Table_1_Effect of diet on molecular relationships between Atlantic cod larval muscle growth dynamics, metabolism, and antioxidant defense system.xlsx |
title_short |
Table_1_Effect of diet on molecular relationships between Atlantic cod larval muscle growth dynamics, metabolism, and antioxidant defense system.xlsx |
title_full |
Table_1_Effect of diet on molecular relationships between Atlantic cod larval muscle growth dynamics, metabolism, and antioxidant defense system.xlsx |
title_fullStr |
Table_1_Effect of diet on molecular relationships between Atlantic cod larval muscle growth dynamics, metabolism, and antioxidant defense system.xlsx |
title_full_unstemmed |
Table_1_Effect of diet on molecular relationships between Atlantic cod larval muscle growth dynamics, metabolism, and antioxidant defense system.xlsx |
title_sort |
table_1_effect of diet on molecular relationships between atlantic cod larval muscle growth dynamics, metabolism, and antioxidant defense system.xlsx |
publishDate |
2022 |
url |
https://doi.org/10.3389/fmars.2022.814022.s001 https://figshare.com/articles/dataset/Table_1_Effect_of_diet_on_molecular_relationships_between_Atlantic_cod_larval_muscle_growth_dynamics_metabolism_and_antioxidant_defense_system_xlsx/20462007 |
genre |
atlantic cod Gadus morhua Copepods Rotifer |
genre_facet |
atlantic cod Gadus morhua Copepods Rotifer |
op_relation |
doi:10.3389/fmars.2022.814022.s001 https://figshare.com/articles/dataset/Table_1_Effect_of_diet_on_molecular_relationships_between_Atlantic_cod_larval_muscle_growth_dynamics_metabolism_and_antioxidant_defense_system_xlsx/20462007 |
op_rights |
CC BY 4.0 |
op_doi |
https://doi.org/10.3389/fmars.2022.814022.s001 |
_version_ |
1809898889749200896 |