DataSheet1_Identification and Characterization of DNA Demethylase Genes and Their Association With Thermal Stress in Wheat (Triticum aestivum L.).PDF
DNA demethylases (dMTases) are essential proteins in plants that regulate DNA methylation levels. The dMTase genes have been explored in a number of plant species, however, members of this family have not been reported in wheat. We identified 12 wheat dMTase genes divided into two subfamilies: repre...
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ftfrontimediafig:oai:figshare.com:article/20357685 2023-05-15T16:02:03+02:00 DataSheet1_Identification and Characterization of DNA Demethylase Genes and Their Association With Thermal Stress in Wheat (Triticum aestivum L.).PDF Vijay Gahlaut Harsha Samtani Tinku Gautam Paramjit Khurana 2022-07-22T08:20:36Z https://doi.org/10.3389/fgene.2022.894020.s001 https://figshare.com/articles/dataset/DataSheet1_Identification_and_Characterization_of_DNA_Demethylase_Genes_and_Their_Association_With_Thermal_Stress_in_Wheat_Triticum_aestivum_L_PDF/20357685 unknown doi:10.3389/fgene.2022.894020.s001 https://figshare.com/articles/dataset/DataSheet1_Identification_and_Characterization_of_DNA_Demethylase_Genes_and_Their_Association_With_Thermal_Stress_in_Wheat_Triticum_aestivum_L_PDF/20357685 CC BY 4.0 CC-BY Genetics Genetic Engineering Biomarkers Developmental Genetics (incl. Sex Determination) Epigenetics (incl. Genome Methylation and Epigenomics) Gene Expression (incl. Microarray and other genome-wide approaches) Genome Structure and Regulation Genomics Genetically Modified Animals Livestock Cloning Gene and Molecular Therapy DNA demethylation bread wheat heat stress response cis-regulatory elements simple sequence repeats (SSR) Dataset 2022 ftfrontimediafig https://doi.org/10.3389/fgene.2022.894020.s001 2022-07-27T23:07:54Z DNA demethylases (dMTases) are essential proteins in plants that regulate DNA methylation levels. The dMTase genes have been explored in a number of plant species, however, members of this family have not been reported in wheat. We identified 12 wheat dMTase genes divided into two subfamilies: repressor of silencing 1 (ROS1) and DEMETER-Like (DML). The TadMTases in the same subfamily or clade in the phylogenetic tree have similar gene structures, protein motifs, and domains. The promoter sequence contains multiple cis-regulatory elements (CREs) that respond to abiotic stress, hormones, and light, suggesting that the majority of TadMTase genes play a role in wheat growth, development, and stress response. The nuclear localization signals (NLSs), subcellular localization, and SRR motifs were also analyzed. The expression profile analyses revealed that TadMTase genes showed differential gene expression patterns in distinct developmental stages and tissues as well as under heat stress (HS). Furthermore, the qRT-PCR analysis revealed that TadMTase gene expression differed amongst wheat cultivars with varying degrees of HS tolerance. Overall, this work contributes to the understanding of the biological function of wheat dMTases and lays the foundation for future investigations. Dataset DML Frontiers: Figshare |
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Frontiers: Figshare |
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ftfrontimediafig |
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unknown |
topic |
Genetics Genetic Engineering Biomarkers Developmental Genetics (incl. Sex Determination) Epigenetics (incl. Genome Methylation and Epigenomics) Gene Expression (incl. Microarray and other genome-wide approaches) Genome Structure and Regulation Genomics Genetically Modified Animals Livestock Cloning Gene and Molecular Therapy DNA demethylation bread wheat heat stress response cis-regulatory elements simple sequence repeats (SSR) |
spellingShingle |
Genetics Genetic Engineering Biomarkers Developmental Genetics (incl. Sex Determination) Epigenetics (incl. Genome Methylation and Epigenomics) Gene Expression (incl. Microarray and other genome-wide approaches) Genome Structure and Regulation Genomics Genetically Modified Animals Livestock Cloning Gene and Molecular Therapy DNA demethylation bread wheat heat stress response cis-regulatory elements simple sequence repeats (SSR) Vijay Gahlaut Harsha Samtani Tinku Gautam Paramjit Khurana DataSheet1_Identification and Characterization of DNA Demethylase Genes and Their Association With Thermal Stress in Wheat (Triticum aestivum L.).PDF |
topic_facet |
Genetics Genetic Engineering Biomarkers Developmental Genetics (incl. Sex Determination) Epigenetics (incl. Genome Methylation and Epigenomics) Gene Expression (incl. Microarray and other genome-wide approaches) Genome Structure and Regulation Genomics Genetically Modified Animals Livestock Cloning Gene and Molecular Therapy DNA demethylation bread wheat heat stress response cis-regulatory elements simple sequence repeats (SSR) |
description |
DNA demethylases (dMTases) are essential proteins in plants that regulate DNA methylation levels. The dMTase genes have been explored in a number of plant species, however, members of this family have not been reported in wheat. We identified 12 wheat dMTase genes divided into two subfamilies: repressor of silencing 1 (ROS1) and DEMETER-Like (DML). The TadMTases in the same subfamily or clade in the phylogenetic tree have similar gene structures, protein motifs, and domains. The promoter sequence contains multiple cis-regulatory elements (CREs) that respond to abiotic stress, hormones, and light, suggesting that the majority of TadMTase genes play a role in wheat growth, development, and stress response. The nuclear localization signals (NLSs), subcellular localization, and SRR motifs were also analyzed. The expression profile analyses revealed that TadMTase genes showed differential gene expression patterns in distinct developmental stages and tissues as well as under heat stress (HS). Furthermore, the qRT-PCR analysis revealed that TadMTase gene expression differed amongst wheat cultivars with varying degrees of HS tolerance. Overall, this work contributes to the understanding of the biological function of wheat dMTases and lays the foundation for future investigations. |
format |
Dataset |
author |
Vijay Gahlaut Harsha Samtani Tinku Gautam Paramjit Khurana |
author_facet |
Vijay Gahlaut Harsha Samtani Tinku Gautam Paramjit Khurana |
author_sort |
Vijay Gahlaut |
title |
DataSheet1_Identification and Characterization of DNA Demethylase Genes and Their Association With Thermal Stress in Wheat (Triticum aestivum L.).PDF |
title_short |
DataSheet1_Identification and Characterization of DNA Demethylase Genes and Their Association With Thermal Stress in Wheat (Triticum aestivum L.).PDF |
title_full |
DataSheet1_Identification and Characterization of DNA Demethylase Genes and Their Association With Thermal Stress in Wheat (Triticum aestivum L.).PDF |
title_fullStr |
DataSheet1_Identification and Characterization of DNA Demethylase Genes and Their Association With Thermal Stress in Wheat (Triticum aestivum L.).PDF |
title_full_unstemmed |
DataSheet1_Identification and Characterization of DNA Demethylase Genes and Their Association With Thermal Stress in Wheat (Triticum aestivum L.).PDF |
title_sort |
datasheet1_identification and characterization of dna demethylase genes and their association with thermal stress in wheat (triticum aestivum l.).pdf |
publishDate |
2022 |
url |
https://doi.org/10.3389/fgene.2022.894020.s001 https://figshare.com/articles/dataset/DataSheet1_Identification_and_Characterization_of_DNA_Demethylase_Genes_and_Their_Association_With_Thermal_Stress_in_Wheat_Triticum_aestivum_L_PDF/20357685 |
genre |
DML |
genre_facet |
DML |
op_relation |
doi:10.3389/fgene.2022.894020.s001 https://figshare.com/articles/dataset/DataSheet1_Identification_and_Characterization_of_DNA_Demethylase_Genes_and_Their_Association_With_Thermal_Stress_in_Wheat_Triticum_aestivum_L_PDF/20357685 |
op_rights |
CC BY 4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fgene.2022.894020.s001 |
_version_ |
1766397678994849792 |