Data_Sheet_1_Comprehensive Metabolic and Taxonomic Reconstruction of an Ancient Microbial Mat From the McMurdo Ice Shelf (Antarctica) by Integrating Genetic, Metaproteomic and Lipid Biomarker Analyses.pdf
Paleobiological reconstructions based on molecular fossils may be limited by degradation processes causing differential preservation of biomolecules, the distinct taxonomic specificity of each biomolecule type, and analytical biases. Here, we combined the analysis of DNA, proteins and lipid biomarke...
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2022
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Online Access: | https://doi.org/10.3389/fmicb.2022.799360.s001 https://figshare.com/articles/dataset/Data_Sheet_1_Comprehensive_Metabolic_and_Taxonomic_Reconstruction_of_an_Ancient_Microbial_Mat_From_the_McMurdo_Ice_Shelf_Antarctica_by_Integrating_Genetic_Metaproteomic_and_Lipid_Biomarker_Analyses_pdf/20338611 |
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ftfrontimediafig:oai:figshare.com:article/20338611 2023-05-15T13:48:56+02:00 Data_Sheet_1_Comprehensive Metabolic and Taxonomic Reconstruction of an Ancient Microbial Mat From the McMurdo Ice Shelf (Antarctica) by Integrating Genetic, Metaproteomic and Lipid Biomarker Analyses.pdf María Ángeles Lezcano Laura Sánchez-García Antonio Quesada Daniel Carrizo Miguel Ángel Fernández-Martínez Erika Cavalcante-Silva Víctor Parro 2022-07-19T14:20:15Z https://doi.org/10.3389/fmicb.2022.799360.s001 https://figshare.com/articles/dataset/Data_Sheet_1_Comprehensive_Metabolic_and_Taxonomic_Reconstruction_of_an_Ancient_Microbial_Mat_From_the_McMurdo_Ice_Shelf_Antarctica_by_Integrating_Genetic_Metaproteomic_and_Lipid_Biomarker_Analyses_pdf/20338611 unknown doi:10.3389/fmicb.2022.799360.s001 https://figshare.com/articles/dataset/Data_Sheet_1_Comprehensive_Metabolic_and_Taxonomic_Reconstruction_of_an_Ancient_Microbial_Mat_From_the_McMurdo_Ice_Shelf_Antarctica_by_Integrating_Genetic_Metaproteomic_and_Lipid_Biomarker_Analyses_pdf/20338611 CC BY 4.0 CC-BY Microbiology Microbial Genetics Microbial Ecology Mycology microbial mat communities microbial metabolism DNA metabarcoding metaproteomics lipid biomarkers McMurdo Ice Shelf Antarctica Dataset 2022 ftfrontimediafig https://doi.org/10.3389/fmicb.2022.799360.s001 2022-07-20T23:03:28Z Paleobiological reconstructions based on molecular fossils may be limited by degradation processes causing differential preservation of biomolecules, the distinct taxonomic specificity of each biomolecule type, and analytical biases. Here, we combined the analysis of DNA, proteins and lipid biomarkers using 16S and 18S rRNA gene metabarcoding, metaproteomics and lipid analysis to reconstruct the taxonomic composition and metabolisms of a desiccated microbial mat from the McMurdo Ice Shelf (MIS) (Antarctica) dated ~1,000 years BP. The different lability, taxonomic resolution and analytical bias of each biomolecule type led to a distinct microbial community profile. DNA analysis showed selective preservation of DNA remnants from the most resistant taxa (e.g., spore-formers). In contrast, the proteins profile revealed microorganisms missed by DNA sequencing, such as Cyanobacteria, and showed a microbial composition similar to fresh microbial mats in the MIS. Lipid hydrocarbons also confirmed Cyanobacteria and suggested the presence of mosses or vascular plant remnants from a period in Antarctica when the climate was warmer (e.g., Mid-Miocene or Eocene). The combined analysis of the three biomolecule types also revealed diverse metabolisms that operated in the microbial mat before desiccation: oxygenic and anoxygenic photosynthesis, nitrogen fixation, nitrification, denitrification, sulfur reduction and oxidation, and methanogenesis. Therefore, the joint analysis of DNA, proteins and lipids resulted in a powerful approach that improved taxonomic and metabolic reconstructions overcoming information gaps derived from using individual biomolecules types. Dataset Antarc* Antarctica Ice Shelf McMurdo Ice Shelf Frontiers: Figshare McMurdo Ice Shelf ENVELOPE(166.500,166.500,-78.000,-78.000) |
institution |
Open Polar |
collection |
Frontiers: Figshare |
op_collection_id |
ftfrontimediafig |
language |
unknown |
topic |
Microbiology Microbial Genetics Microbial Ecology Mycology microbial mat communities microbial metabolism DNA metabarcoding metaproteomics lipid biomarkers McMurdo Ice Shelf Antarctica |
spellingShingle |
Microbiology Microbial Genetics Microbial Ecology Mycology microbial mat communities microbial metabolism DNA metabarcoding metaproteomics lipid biomarkers McMurdo Ice Shelf Antarctica María Ángeles Lezcano Laura Sánchez-García Antonio Quesada Daniel Carrizo Miguel Ángel Fernández-Martínez Erika Cavalcante-Silva Víctor Parro Data_Sheet_1_Comprehensive Metabolic and Taxonomic Reconstruction of an Ancient Microbial Mat From the McMurdo Ice Shelf (Antarctica) by Integrating Genetic, Metaproteomic and Lipid Biomarker Analyses.pdf |
topic_facet |
Microbiology Microbial Genetics Microbial Ecology Mycology microbial mat communities microbial metabolism DNA metabarcoding metaproteomics lipid biomarkers McMurdo Ice Shelf Antarctica |
description |
Paleobiological reconstructions based on molecular fossils may be limited by degradation processes causing differential preservation of biomolecules, the distinct taxonomic specificity of each biomolecule type, and analytical biases. Here, we combined the analysis of DNA, proteins and lipid biomarkers using 16S and 18S rRNA gene metabarcoding, metaproteomics and lipid analysis to reconstruct the taxonomic composition and metabolisms of a desiccated microbial mat from the McMurdo Ice Shelf (MIS) (Antarctica) dated ~1,000 years BP. The different lability, taxonomic resolution and analytical bias of each biomolecule type led to a distinct microbial community profile. DNA analysis showed selective preservation of DNA remnants from the most resistant taxa (e.g., spore-formers). In contrast, the proteins profile revealed microorganisms missed by DNA sequencing, such as Cyanobacteria, and showed a microbial composition similar to fresh microbial mats in the MIS. Lipid hydrocarbons also confirmed Cyanobacteria and suggested the presence of mosses or vascular plant remnants from a period in Antarctica when the climate was warmer (e.g., Mid-Miocene or Eocene). The combined analysis of the three biomolecule types also revealed diverse metabolisms that operated in the microbial mat before desiccation: oxygenic and anoxygenic photosynthesis, nitrogen fixation, nitrification, denitrification, sulfur reduction and oxidation, and methanogenesis. Therefore, the joint analysis of DNA, proteins and lipids resulted in a powerful approach that improved taxonomic and metabolic reconstructions overcoming information gaps derived from using individual biomolecules types. |
format |
Dataset |
author |
María Ángeles Lezcano Laura Sánchez-García Antonio Quesada Daniel Carrizo Miguel Ángel Fernández-Martínez Erika Cavalcante-Silva Víctor Parro |
author_facet |
María Ángeles Lezcano Laura Sánchez-García Antonio Quesada Daniel Carrizo Miguel Ángel Fernández-Martínez Erika Cavalcante-Silva Víctor Parro |
author_sort |
María Ángeles Lezcano |
title |
Data_Sheet_1_Comprehensive Metabolic and Taxonomic Reconstruction of an Ancient Microbial Mat From the McMurdo Ice Shelf (Antarctica) by Integrating Genetic, Metaproteomic and Lipid Biomarker Analyses.pdf |
title_short |
Data_Sheet_1_Comprehensive Metabolic and Taxonomic Reconstruction of an Ancient Microbial Mat From the McMurdo Ice Shelf (Antarctica) by Integrating Genetic, Metaproteomic and Lipid Biomarker Analyses.pdf |
title_full |
Data_Sheet_1_Comprehensive Metabolic and Taxonomic Reconstruction of an Ancient Microbial Mat From the McMurdo Ice Shelf (Antarctica) by Integrating Genetic, Metaproteomic and Lipid Biomarker Analyses.pdf |
title_fullStr |
Data_Sheet_1_Comprehensive Metabolic and Taxonomic Reconstruction of an Ancient Microbial Mat From the McMurdo Ice Shelf (Antarctica) by Integrating Genetic, Metaproteomic and Lipid Biomarker Analyses.pdf |
title_full_unstemmed |
Data_Sheet_1_Comprehensive Metabolic and Taxonomic Reconstruction of an Ancient Microbial Mat From the McMurdo Ice Shelf (Antarctica) by Integrating Genetic, Metaproteomic and Lipid Biomarker Analyses.pdf |
title_sort |
data_sheet_1_comprehensive metabolic and taxonomic reconstruction of an ancient microbial mat from the mcmurdo ice shelf (antarctica) by integrating genetic, metaproteomic and lipid biomarker analyses.pdf |
publishDate |
2022 |
url |
https://doi.org/10.3389/fmicb.2022.799360.s001 https://figshare.com/articles/dataset/Data_Sheet_1_Comprehensive_Metabolic_and_Taxonomic_Reconstruction_of_an_Ancient_Microbial_Mat_From_the_McMurdo_Ice_Shelf_Antarctica_by_Integrating_Genetic_Metaproteomic_and_Lipid_Biomarker_Analyses_pdf/20338611 |
long_lat |
ENVELOPE(166.500,166.500,-78.000,-78.000) |
geographic |
McMurdo Ice Shelf |
geographic_facet |
McMurdo Ice Shelf |
genre |
Antarc* Antarctica Ice Shelf McMurdo Ice Shelf |
genre_facet |
Antarc* Antarctica Ice Shelf McMurdo Ice Shelf |
op_relation |
doi:10.3389/fmicb.2022.799360.s001 https://figshare.com/articles/dataset/Data_Sheet_1_Comprehensive_Metabolic_and_Taxonomic_Reconstruction_of_an_Ancient_Microbial_Mat_From_the_McMurdo_Ice_Shelf_Antarctica_by_Integrating_Genetic_Metaproteomic_and_Lipid_Biomarker_Analyses_pdf/20338611 |
op_rights |
CC BY 4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fmicb.2022.799360.s001 |
_version_ |
1766249993740484608 |