Table_5_Ubiquity and Diversity of Cold Adapted Denitrifying Bacteria Isolated From Diverse Antarctic Ecosystems.DOCX
Nitrogen cycle has been poorly investigated in Antarctic ecosystems. In particular, how extreme conditions of low temperature, dryness, and high radiation select the microorganisms involved in the cycle is not yet understood. Denitrification is an important step in the nitrogen cycle in which nitrat...
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ftfrontimediafig:oai:figshare.com:article/20328486 2023-05-15T13:48:56+02:00 Table_5_Ubiquity and Diversity of Cold Adapted Denitrifying Bacteria Isolated From Diverse Antarctic Ecosystems.DOCX Angela Cabezas Gastón Azziz Patricia Bovio-Winkler Laura Fuentes Lucía Braga Jorge Wenzel Silvia Sabaris Silvana Tarlera Claudia Etchebehere 2022-07-18T04:56:30Z https://doi.org/10.3389/fmicb.2022.827228.s005 https://figshare.com/articles/dataset/Table_5_Ubiquity_and_Diversity_of_Cold_Adapted_Denitrifying_Bacteria_Isolated_From_Diverse_Antarctic_Ecosystems_DOCX/20328486 unknown doi:10.3389/fmicb.2022.827228.s005 https://figshare.com/articles/dataset/Table_5_Ubiquity_and_Diversity_of_Cold_Adapted_Denitrifying_Bacteria_Isolated_From_Diverse_Antarctic_Ecosystems_DOCX/20328486 CC BY 4.0 CC-BY Microbiology Microbial Genetics Microbial Ecology Mycology denitrification isolation Antarctica denitrifying bacteria nitrogen cycle cold environments Dataset 2022 ftfrontimediafig https://doi.org/10.3389/fmicb.2022.827228.s005 2022-07-20T23:05:25Z Nitrogen cycle has been poorly investigated in Antarctic ecosystems. In particular, how extreme conditions of low temperature, dryness, and high radiation select the microorganisms involved in the cycle is not yet understood. Denitrification is an important step in the nitrogen cycle in which nitrate is reduced stepwise to the gases NO, N 2 O, and N 2 . Denitrification is carried out by a wide group of microorganisms spread in the phylogenetic tree. The aim of this work was to isolate and characterize denitrifying bacteria present in different cold environments from Antarctica. Bacterial isolates were obtained from lake, meltwater, sea, glacier ice, ornithogenic soil, and penguin feces samples from King George Island, Fildes peninsula in the Antarctic. Samples were taken during the deicing season in five sampling campaigns. From all the samples we were able to isolate denitrifying strains. A total of 199 bacterial isolates with the capacity to grow in anaerobic mineral media reducing nitrate at 4°C were obtained. The characterization of the isolates by 16S rRNA gene sequence analysis showed a high predominance of the genus Pseudomonas, followed by Janthinobacterium, Flavobacterium, Psychrobacter, and Yersinia. Other minor genera detected were Cryobacterium, Iodobacter, Kaistella, and Carnobacterium. The capacity to denitrify was not previously described for most of the bacteria related to our isolates and in many of them denitrifying genes were not present suggesting the presence of new genes in this extreme environment. Our work demonstrates the ubiquity of denitrification in the Maritime Antarctica and gives important information linking denitrification at cold temperature with taxa in an unequivocal way. Dataset Antarc* Antarctic Antarctica King George Island Frontiers: Figshare Antarctic The Antarctic King George Island Fildes ENVELOPE(-58.817,-58.817,-62.217,-62.217) Fildes peninsula ENVELOPE(-58.948,-58.948,-62.182,-62.182) |
institution |
Open Polar |
collection |
Frontiers: Figshare |
op_collection_id |
ftfrontimediafig |
language |
unknown |
topic |
Microbiology Microbial Genetics Microbial Ecology Mycology denitrification isolation Antarctica denitrifying bacteria nitrogen cycle cold environments |
spellingShingle |
Microbiology Microbial Genetics Microbial Ecology Mycology denitrification isolation Antarctica denitrifying bacteria nitrogen cycle cold environments Angela Cabezas Gastón Azziz Patricia Bovio-Winkler Laura Fuentes Lucía Braga Jorge Wenzel Silvia Sabaris Silvana Tarlera Claudia Etchebehere Table_5_Ubiquity and Diversity of Cold Adapted Denitrifying Bacteria Isolated From Diverse Antarctic Ecosystems.DOCX |
topic_facet |
Microbiology Microbial Genetics Microbial Ecology Mycology denitrification isolation Antarctica denitrifying bacteria nitrogen cycle cold environments |
description |
Nitrogen cycle has been poorly investigated in Antarctic ecosystems. In particular, how extreme conditions of low temperature, dryness, and high radiation select the microorganisms involved in the cycle is not yet understood. Denitrification is an important step in the nitrogen cycle in which nitrate is reduced stepwise to the gases NO, N 2 O, and N 2 . Denitrification is carried out by a wide group of microorganisms spread in the phylogenetic tree. The aim of this work was to isolate and characterize denitrifying bacteria present in different cold environments from Antarctica. Bacterial isolates were obtained from lake, meltwater, sea, glacier ice, ornithogenic soil, and penguin feces samples from King George Island, Fildes peninsula in the Antarctic. Samples were taken during the deicing season in five sampling campaigns. From all the samples we were able to isolate denitrifying strains. A total of 199 bacterial isolates with the capacity to grow in anaerobic mineral media reducing nitrate at 4°C were obtained. The characterization of the isolates by 16S rRNA gene sequence analysis showed a high predominance of the genus Pseudomonas, followed by Janthinobacterium, Flavobacterium, Psychrobacter, and Yersinia. Other minor genera detected were Cryobacterium, Iodobacter, Kaistella, and Carnobacterium. The capacity to denitrify was not previously described for most of the bacteria related to our isolates and in many of them denitrifying genes were not present suggesting the presence of new genes in this extreme environment. Our work demonstrates the ubiquity of denitrification in the Maritime Antarctica and gives important information linking denitrification at cold temperature with taxa in an unequivocal way. |
format |
Dataset |
author |
Angela Cabezas Gastón Azziz Patricia Bovio-Winkler Laura Fuentes Lucía Braga Jorge Wenzel Silvia Sabaris Silvana Tarlera Claudia Etchebehere |
author_facet |
Angela Cabezas Gastón Azziz Patricia Bovio-Winkler Laura Fuentes Lucía Braga Jorge Wenzel Silvia Sabaris Silvana Tarlera Claudia Etchebehere |
author_sort |
Angela Cabezas |
title |
Table_5_Ubiquity and Diversity of Cold Adapted Denitrifying Bacteria Isolated From Diverse Antarctic Ecosystems.DOCX |
title_short |
Table_5_Ubiquity and Diversity of Cold Adapted Denitrifying Bacteria Isolated From Diverse Antarctic Ecosystems.DOCX |
title_full |
Table_5_Ubiquity and Diversity of Cold Adapted Denitrifying Bacteria Isolated From Diverse Antarctic Ecosystems.DOCX |
title_fullStr |
Table_5_Ubiquity and Diversity of Cold Adapted Denitrifying Bacteria Isolated From Diverse Antarctic Ecosystems.DOCX |
title_full_unstemmed |
Table_5_Ubiquity and Diversity of Cold Adapted Denitrifying Bacteria Isolated From Diverse Antarctic Ecosystems.DOCX |
title_sort |
table_5_ubiquity and diversity of cold adapted denitrifying bacteria isolated from diverse antarctic ecosystems.docx |
publishDate |
2022 |
url |
https://doi.org/10.3389/fmicb.2022.827228.s005 https://figshare.com/articles/dataset/Table_5_Ubiquity_and_Diversity_of_Cold_Adapted_Denitrifying_Bacteria_Isolated_From_Diverse_Antarctic_Ecosystems_DOCX/20328486 |
long_lat |
ENVELOPE(-58.817,-58.817,-62.217,-62.217) ENVELOPE(-58.948,-58.948,-62.182,-62.182) |
geographic |
Antarctic The Antarctic King George Island Fildes Fildes peninsula |
geographic_facet |
Antarctic The Antarctic King George Island Fildes Fildes peninsula |
genre |
Antarc* Antarctic Antarctica King George Island |
genre_facet |
Antarc* Antarctic Antarctica King George Island |
op_relation |
doi:10.3389/fmicb.2022.827228.s005 https://figshare.com/articles/dataset/Table_5_Ubiquity_and_Diversity_of_Cold_Adapted_Denitrifying_Bacteria_Isolated_From_Diverse_Antarctic_Ecosystems_DOCX/20328486 |
op_rights |
CC BY 4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fmicb.2022.827228.s005 |
_version_ |
1766249994508042240 |