Image_1_RGD-Labeled Hemocytes With High Migration Activity Display a Potential Immunomodulatory Role in the Pacific Oyster Crassostrea gigas.tif

Immunocyte migration to infection sites is important for host cellular defense, but the main types of migrating hemocytes and their mechanisms against pathogen invasions are unclear in invertebrates. In the present study, a population of hemocytes in the Pacific oyster Crassostrea gigas labeled with...

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Main Authors: Zhao Lv, Limei Qiu, Weilin Wang, Zhaoqun Liu, Qing Liu, Lingling Wang, Linsheng Song
Format: Still Image
Language:unknown
Published: 2022
Subjects:
Online Access:https://doi.org/10.3389/fimmu.2022.914899.s001
https://figshare.com/articles/figure/Image_1_RGD-Labeled_Hemocytes_With_High_Migration_Activity_Display_a_Potential_Immunomodulatory_Role_in_the_Pacific_Oyster_Crassostrea_gigas_tif/20225286
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spelling ftfrontimediafig:oai:figshare.com:article/20225286 2023-05-15T15:58:57+02:00 Image_1_RGD-Labeled Hemocytes With High Migration Activity Display a Potential Immunomodulatory Role in the Pacific Oyster Crassostrea gigas.tif Zhao Lv Limei Qiu Weilin Wang Zhaoqun Liu Qing Liu Lingling Wang Linsheng Song 2022-07-05T04:44:42Z https://doi.org/10.3389/fimmu.2022.914899.s001 https://figshare.com/articles/figure/Image_1_RGD-Labeled_Hemocytes_With_High_Migration_Activity_Display_a_Potential_Immunomodulatory_Role_in_the_Pacific_Oyster_Crassostrea_gigas_tif/20225286 unknown doi:10.3389/fimmu.2022.914899.s001 https://figshare.com/articles/figure/Image_1_RGD-Labeled_Hemocytes_With_High_Migration_Activity_Display_a_Potential_Immunomodulatory_Role_in_the_Pacific_Oyster_Crassostrea_gigas_tif/20225286 CC BY 4.0 CC-BY Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology C. gigas RGD labeled hemocytes migration activity immunomodulatory antimicrobial immunity Image Figure 2022 ftfrontimediafig https://doi.org/10.3389/fimmu.2022.914899.s001 2022-07-06T23:05:28Z Immunocyte migration to infection sites is important for host cellular defense, but the main types of migrating hemocytes and their mechanisms against pathogen invasions are unclear in invertebrates. In the present study, a population of hemocytes in the Pacific oyster Crassostrea gigas labeled with a fluorescein isothiocyanate (FITC)-conjugated Arg-Gly-Asp (RGD)-containing peptide was sorted. RGD + hemocytes were characterized by a smaller cell size and cytoplasmic-nucleo ratio, fewer cytoplasmic granules, and higher levels of myeloperoxidase, reactive oxygen species, and intracellular free calcium concentration. RGD + hemocytes exhibited a high level of migration activity, which was further induced after V. splendidus infection. Transcriptome analysis revealed that RGD + hemocytes highly expressed a series of migration-related genes, which together with migration-promoting genes were significantly upregulated after V. splendidus infection. The neuroendocrine system was also proven to regulate the migration activity of RGD + hemocytes, especially with the excitatory neuroendocrine factor dopamine, which promoted migration activity as confirmed by receptor blocking assays. Meanwhile, RGD + hemocytes could highly express immunomodulatory factor interleukin (IL)-17s and their receptor genes, which was positively related to the production of antimicrobial peptides in whole hemocytes after V. splendidus infection. Collectively, this study identified a specific hemocyte population, i.e., RGD + hemocytes, that shows high migration activity in response to pathogen infection and exerts a potential immunomodulatory role by highly expressing IL-17s that might enhance the hemocytes’ antimicrobial peptide production in oysters. Still Image Crassostrea gigas Pacific oyster Frontiers: Figshare Pacific
institution Open Polar
collection Frontiers: Figshare
op_collection_id ftfrontimediafig
language unknown
topic Immunology
Applied Immunology (incl. Antibody Engineering
Xenotransplantation and T-cell Therapies)
Autoimmunity
Cellular Immunology
Humoural Immunology and Immunochemistry
Immunogenetics (incl. Genetic Immunology)
Innate Immunity
Transplantation Immunology
Tumour Immunology
Immunology not elsewhere classified
Genetic Immunology
Animal Immunology
Veterinary Immunology
C. gigas
RGD labeled hemocytes
migration activity
immunomodulatory
antimicrobial immunity
spellingShingle Immunology
Applied Immunology (incl. Antibody Engineering
Xenotransplantation and T-cell Therapies)
Autoimmunity
Cellular Immunology
Humoural Immunology and Immunochemistry
Immunogenetics (incl. Genetic Immunology)
Innate Immunity
Transplantation Immunology
Tumour Immunology
Immunology not elsewhere classified
Genetic Immunology
Animal Immunology
Veterinary Immunology
C. gigas
RGD labeled hemocytes
migration activity
immunomodulatory
antimicrobial immunity
Zhao Lv
Limei Qiu
Weilin Wang
Zhaoqun Liu
Qing Liu
Lingling Wang
Linsheng Song
Image_1_RGD-Labeled Hemocytes With High Migration Activity Display a Potential Immunomodulatory Role in the Pacific Oyster Crassostrea gigas.tif
topic_facet Immunology
Applied Immunology (incl. Antibody Engineering
Xenotransplantation and T-cell Therapies)
Autoimmunity
Cellular Immunology
Humoural Immunology and Immunochemistry
Immunogenetics (incl. Genetic Immunology)
Innate Immunity
Transplantation Immunology
Tumour Immunology
Immunology not elsewhere classified
Genetic Immunology
Animal Immunology
Veterinary Immunology
C. gigas
RGD labeled hemocytes
migration activity
immunomodulatory
antimicrobial immunity
description Immunocyte migration to infection sites is important for host cellular defense, but the main types of migrating hemocytes and their mechanisms against pathogen invasions are unclear in invertebrates. In the present study, a population of hemocytes in the Pacific oyster Crassostrea gigas labeled with a fluorescein isothiocyanate (FITC)-conjugated Arg-Gly-Asp (RGD)-containing peptide was sorted. RGD + hemocytes were characterized by a smaller cell size and cytoplasmic-nucleo ratio, fewer cytoplasmic granules, and higher levels of myeloperoxidase, reactive oxygen species, and intracellular free calcium concentration. RGD + hemocytes exhibited a high level of migration activity, which was further induced after V. splendidus infection. Transcriptome analysis revealed that RGD + hemocytes highly expressed a series of migration-related genes, which together with migration-promoting genes were significantly upregulated after V. splendidus infection. The neuroendocrine system was also proven to regulate the migration activity of RGD + hemocytes, especially with the excitatory neuroendocrine factor dopamine, which promoted migration activity as confirmed by receptor blocking assays. Meanwhile, RGD + hemocytes could highly express immunomodulatory factor interleukin (IL)-17s and their receptor genes, which was positively related to the production of antimicrobial peptides in whole hemocytes after V. splendidus infection. Collectively, this study identified a specific hemocyte population, i.e., RGD + hemocytes, that shows high migration activity in response to pathogen infection and exerts a potential immunomodulatory role by highly expressing IL-17s that might enhance the hemocytes’ antimicrobial peptide production in oysters.
format Still Image
author Zhao Lv
Limei Qiu
Weilin Wang
Zhaoqun Liu
Qing Liu
Lingling Wang
Linsheng Song
author_facet Zhao Lv
Limei Qiu
Weilin Wang
Zhaoqun Liu
Qing Liu
Lingling Wang
Linsheng Song
author_sort Zhao Lv
title Image_1_RGD-Labeled Hemocytes With High Migration Activity Display a Potential Immunomodulatory Role in the Pacific Oyster Crassostrea gigas.tif
title_short Image_1_RGD-Labeled Hemocytes With High Migration Activity Display a Potential Immunomodulatory Role in the Pacific Oyster Crassostrea gigas.tif
title_full Image_1_RGD-Labeled Hemocytes With High Migration Activity Display a Potential Immunomodulatory Role in the Pacific Oyster Crassostrea gigas.tif
title_fullStr Image_1_RGD-Labeled Hemocytes With High Migration Activity Display a Potential Immunomodulatory Role in the Pacific Oyster Crassostrea gigas.tif
title_full_unstemmed Image_1_RGD-Labeled Hemocytes With High Migration Activity Display a Potential Immunomodulatory Role in the Pacific Oyster Crassostrea gigas.tif
title_sort image_1_rgd-labeled hemocytes with high migration activity display a potential immunomodulatory role in the pacific oyster crassostrea gigas.tif
publishDate 2022
url https://doi.org/10.3389/fimmu.2022.914899.s001
https://figshare.com/articles/figure/Image_1_RGD-Labeled_Hemocytes_With_High_Migration_Activity_Display_a_Potential_Immunomodulatory_Role_in_the_Pacific_Oyster_Crassostrea_gigas_tif/20225286
geographic Pacific
geographic_facet Pacific
genre Crassostrea gigas
Pacific oyster
genre_facet Crassostrea gigas
Pacific oyster
op_relation doi:10.3389/fimmu.2022.914899.s001
https://figshare.com/articles/figure/Image_1_RGD-Labeled_Hemocytes_With_High_Migration_Activity_Display_a_Potential_Immunomodulatory_Role_in_the_Pacific_Oyster_Crassostrea_gigas_tif/20225286
op_rights CC BY 4.0
op_rightsnorm CC-BY
op_doi https://doi.org/10.3389/fimmu.2022.914899.s001
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