Table_1_A Comparative Study of Mini-FLOTAC With Traditional Coprological Techniques in the Analysis of Cetacean Fecal Samples.DOCX
Free-ranging cetaceans are considered sentinels for the marine ecosystem's health. New and non-invasive methods have been set up for the collection of fecal samples from free-ranging big whales at sea, permitting to gain an excellent epidemiological picture of parasitic infections in wild popul...
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ftfrontimediafig:oai:figshare.com:article/20156564 2023-05-15T15:36:43+02:00 Table_1_A Comparative Study of Mini-FLOTAC With Traditional Coprological Techniques in the Analysis of Cetacean Fecal Samples.DOCX Federica Marcer Rudi Cassini Nancy Parisotto Cinzia Tessarin Erica Marchiori 2022-06-27T05:25:54Z https://doi.org/10.3389/fvets.2022.908486.s001 https://figshare.com/articles/dataset/Table_1_A_Comparative_Study_of_Mini-FLOTAC_With_Traditional_Coprological_Techniques_in_the_Analysis_of_Cetacean_Fecal_Samples_DOCX/20156564 unknown doi:10.3389/fvets.2022.908486.s001 https://figshare.com/articles/dataset/Table_1_A_Comparative_Study_of_Mini-FLOTAC_With_Traditional_Coprological_Techniques_in_the_Analysis_of_Cetacean_Fecal_Samples_DOCX/20156564 CC BY 4.0 CC-BY Animal Systematics and Taxonomy Animal Physiology - Biophysics Animal Physiology - Cell Animal Physiology - Systems Animal Behaviour Animal Cell and Molecular Biology Animal Developmental and Reproductive Biology Animal Immunology Animal Neurobiology Animal Physiological Ecology Animal Structure and Function Veterinary Anaesthesiology and Intensive Care Veterinary Anatomy and Physiology Veterinary Diagnosis and Diagnostics Veterinary Epidemiology Veterinary Immunology Veterinary Medicine Veterinary Microbiology (excl. Virology) Veterinary Parasitology Veterinary Pathology Veterinary Pharmacology Veterinary Surgery Veterinary Virology Veterinary Sciences not elsewhere classified cetacean Mini-FLOTAC copromicroscopy sensitivity helminths Mediterranean Sea Dataset 2022 ftfrontimediafig https://doi.org/10.3389/fvets.2022.908486.s001 2022-06-29T23:06:21Z Free-ranging cetaceans are considered sentinels for the marine ecosystem's health. New and non-invasive methods have been set up for the collection of fecal samples from free-ranging big whales at sea, permitting to gain an excellent epidemiological picture of parasitic infections in wild populations. To select the best protocol to be used for copromicroscopic examination in cetaceans stool samples, we evaluated the sensitivity of two commonly used techniques, i.e., a sedimentation-floatation method and the Mini-FLOTAC through validation by helminth isolation from the digestive tract. For this aim, gastrointestinal content and fecal samples were collected during necropsy from 44 cetaceans, including bottlenose dolphins (Tursiops truncatus), striped dolphins (Stenella coeruleoalba), sperm whales (Physeter macrocephalus), Risso's dolphins (Grampus griseus), Cuvier's beaked whales (Ziphius cavirostris), pilot whale (Globicephala melas), and fin whales (Balaenoptera physalus). Helminths were recovered through washing and filtering of the gastrointestinal contents and morphologically identified. Copromicroscopic examinations were performed on formalin-preserved fecal samples, using a sodium nitrate, sodium thiosulphate, and sucrose solution (s.g. = 1.450) for both methods. Helminths belonging to 9 taxa (i.e., the trematodes Synthesium tursionis, Synthesium delamurei, Campula palliata, Braunina cordiformis, Pholeter gastrophilus, the nematode Anisakis sp., cestodes of the family Tetrabothriidae and the acanthocephalan Bolbosoma sp.) were isolated. Eggs referable to the same taxa, with the exception of cestodes, were found in copromicroscopic analyses. Sensitivity of the Mini-FLOTAC method appeared higher or equal for all taxa, proving superior to the sedimentation-flotation method for the detection of all except Anisakis sp. The concordance of the two tests indeed revealed a moderate to perfect agreement (kappa values 0.42–1). Not excluding the limitations inherent to the techniques themselves, explanations for ... Dataset Balaenoptera physalus Physeter macrocephalus Frontiers: Figshare |
institution |
Open Polar |
collection |
Frontiers: Figshare |
op_collection_id |
ftfrontimediafig |
language |
unknown |
topic |
Animal Systematics and Taxonomy Animal Physiology - Biophysics Animal Physiology - Cell Animal Physiology - Systems Animal Behaviour Animal Cell and Molecular Biology Animal Developmental and Reproductive Biology Animal Immunology Animal Neurobiology Animal Physiological Ecology Animal Structure and Function Veterinary Anaesthesiology and Intensive Care Veterinary Anatomy and Physiology Veterinary Diagnosis and Diagnostics Veterinary Epidemiology Veterinary Immunology Veterinary Medicine Veterinary Microbiology (excl. Virology) Veterinary Parasitology Veterinary Pathology Veterinary Pharmacology Veterinary Surgery Veterinary Virology Veterinary Sciences not elsewhere classified cetacean Mini-FLOTAC copromicroscopy sensitivity helminths Mediterranean Sea |
spellingShingle |
Animal Systematics and Taxonomy Animal Physiology - Biophysics Animal Physiology - Cell Animal Physiology - Systems Animal Behaviour Animal Cell and Molecular Biology Animal Developmental and Reproductive Biology Animal Immunology Animal Neurobiology Animal Physiological Ecology Animal Structure and Function Veterinary Anaesthesiology and Intensive Care Veterinary Anatomy and Physiology Veterinary Diagnosis and Diagnostics Veterinary Epidemiology Veterinary Immunology Veterinary Medicine Veterinary Microbiology (excl. Virology) Veterinary Parasitology Veterinary Pathology Veterinary Pharmacology Veterinary Surgery Veterinary Virology Veterinary Sciences not elsewhere classified cetacean Mini-FLOTAC copromicroscopy sensitivity helminths Mediterranean Sea Federica Marcer Rudi Cassini Nancy Parisotto Cinzia Tessarin Erica Marchiori Table_1_A Comparative Study of Mini-FLOTAC With Traditional Coprological Techniques in the Analysis of Cetacean Fecal Samples.DOCX |
topic_facet |
Animal Systematics and Taxonomy Animal Physiology - Biophysics Animal Physiology - Cell Animal Physiology - Systems Animal Behaviour Animal Cell and Molecular Biology Animal Developmental and Reproductive Biology Animal Immunology Animal Neurobiology Animal Physiological Ecology Animal Structure and Function Veterinary Anaesthesiology and Intensive Care Veterinary Anatomy and Physiology Veterinary Diagnosis and Diagnostics Veterinary Epidemiology Veterinary Immunology Veterinary Medicine Veterinary Microbiology (excl. Virology) Veterinary Parasitology Veterinary Pathology Veterinary Pharmacology Veterinary Surgery Veterinary Virology Veterinary Sciences not elsewhere classified cetacean Mini-FLOTAC copromicroscopy sensitivity helminths Mediterranean Sea |
description |
Free-ranging cetaceans are considered sentinels for the marine ecosystem's health. New and non-invasive methods have been set up for the collection of fecal samples from free-ranging big whales at sea, permitting to gain an excellent epidemiological picture of parasitic infections in wild populations. To select the best protocol to be used for copromicroscopic examination in cetaceans stool samples, we evaluated the sensitivity of two commonly used techniques, i.e., a sedimentation-floatation method and the Mini-FLOTAC through validation by helminth isolation from the digestive tract. For this aim, gastrointestinal content and fecal samples were collected during necropsy from 44 cetaceans, including bottlenose dolphins (Tursiops truncatus), striped dolphins (Stenella coeruleoalba), sperm whales (Physeter macrocephalus), Risso's dolphins (Grampus griseus), Cuvier's beaked whales (Ziphius cavirostris), pilot whale (Globicephala melas), and fin whales (Balaenoptera physalus). Helminths were recovered through washing and filtering of the gastrointestinal contents and morphologically identified. Copromicroscopic examinations were performed on formalin-preserved fecal samples, using a sodium nitrate, sodium thiosulphate, and sucrose solution (s.g. = 1.450) for both methods. Helminths belonging to 9 taxa (i.e., the trematodes Synthesium tursionis, Synthesium delamurei, Campula palliata, Braunina cordiformis, Pholeter gastrophilus, the nematode Anisakis sp., cestodes of the family Tetrabothriidae and the acanthocephalan Bolbosoma sp.) were isolated. Eggs referable to the same taxa, with the exception of cestodes, were found in copromicroscopic analyses. Sensitivity of the Mini-FLOTAC method appeared higher or equal for all taxa, proving superior to the sedimentation-flotation method for the detection of all except Anisakis sp. The concordance of the two tests indeed revealed a moderate to perfect agreement (kappa values 0.42–1). Not excluding the limitations inherent to the techniques themselves, explanations for ... |
format |
Dataset |
author |
Federica Marcer Rudi Cassini Nancy Parisotto Cinzia Tessarin Erica Marchiori |
author_facet |
Federica Marcer Rudi Cassini Nancy Parisotto Cinzia Tessarin Erica Marchiori |
author_sort |
Federica Marcer |
title |
Table_1_A Comparative Study of Mini-FLOTAC With Traditional Coprological Techniques in the Analysis of Cetacean Fecal Samples.DOCX |
title_short |
Table_1_A Comparative Study of Mini-FLOTAC With Traditional Coprological Techniques in the Analysis of Cetacean Fecal Samples.DOCX |
title_full |
Table_1_A Comparative Study of Mini-FLOTAC With Traditional Coprological Techniques in the Analysis of Cetacean Fecal Samples.DOCX |
title_fullStr |
Table_1_A Comparative Study of Mini-FLOTAC With Traditional Coprological Techniques in the Analysis of Cetacean Fecal Samples.DOCX |
title_full_unstemmed |
Table_1_A Comparative Study of Mini-FLOTAC With Traditional Coprological Techniques in the Analysis of Cetacean Fecal Samples.DOCX |
title_sort |
table_1_a comparative study of mini-flotac with traditional coprological techniques in the analysis of cetacean fecal samples.docx |
publishDate |
2022 |
url |
https://doi.org/10.3389/fvets.2022.908486.s001 https://figshare.com/articles/dataset/Table_1_A_Comparative_Study_of_Mini-FLOTAC_With_Traditional_Coprological_Techniques_in_the_Analysis_of_Cetacean_Fecal_Samples_DOCX/20156564 |
genre |
Balaenoptera physalus Physeter macrocephalus |
genre_facet |
Balaenoptera physalus Physeter macrocephalus |
op_relation |
doi:10.3389/fvets.2022.908486.s001 https://figshare.com/articles/dataset/Table_1_A_Comparative_Study_of_Mini-FLOTAC_With_Traditional_Coprological_Techniques_in_the_Analysis_of_Cetacean_Fecal_Samples_DOCX/20156564 |
op_rights |
CC BY 4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fvets.2022.908486.s001 |
_version_ |
1766367090122424320 |