Data_Sheet_1_Identification of a New Antifungal Peptide W1 From a Marine Bacillus amyloliquefaciens Reveals Its Potential in Controlling Fungal Plant Diseases.docx
A bacterium, Bacillus amyloliquefaciens W0101, isolated from the Arctic Ocean, showed potent antifungal activity against several plant pathogenic fungi. An antifungal peptide W1, with a molecular weight of approximately 2.4 kDa, was purified from the culture supernatant of the strain W0101 using ion...
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Online Access: | https://doi.org/10.3389/fmicb.2022.922454.s001 https://figshare.com/articles/dataset/Data_Sheet_1_Identification_of_a_New_Antifungal_Peptide_W1_From_a_Marine_Bacillus_amyloliquefaciens_Reveals_Its_Potential_in_Controlling_Fungal_Plant_Diseases_docx/20066435 |
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ftfrontimediafig:oai:figshare.com:article/20066435 2023-05-15T14:57:55+02:00 Data_Sheet_1_Identification of a New Antifungal Peptide W1 From a Marine Bacillus amyloliquefaciens Reveals Its Potential in Controlling Fungal Plant Diseases.docx Qiao Wen Ruizhe Liu Zhenxiao Ouyang Tianliang He Weini Zhang Xinhua Chen 2022-06-14T11:33:12Z https://doi.org/10.3389/fmicb.2022.922454.s001 https://figshare.com/articles/dataset/Data_Sheet_1_Identification_of_a_New_Antifungal_Peptide_W1_From_a_Marine_Bacillus_amyloliquefaciens_Reveals_Its_Potential_in_Controlling_Fungal_Plant_Diseases_docx/20066435 unknown doi:10.3389/fmicb.2022.922454.s001 https://figshare.com/articles/dataset/Data_Sheet_1_Identification_of_a_New_Antifungal_Peptide_W1_From_a_Marine_Bacillus_amyloliquefaciens_Reveals_Its_Potential_in_Controlling_Fungal_Plant_Diseases_docx/20066435 CC BY 4.0 CC-BY Microbiology Microbial Genetics Microbial Ecology Mycology plant pathogenic fungi Bacillus amyloliquefaciens antifungal peptide antifungal activity Arctic Ocean Dataset 2022 ftfrontimediafig https://doi.org/10.3389/fmicb.2022.922454.s001 2022-06-15T23:04:49Z A bacterium, Bacillus amyloliquefaciens W0101, isolated from the Arctic Ocean, showed potent antifungal activity against several plant pathogenic fungi. An antifungal peptide W1, with a molecular weight of approximately 2.4 kDa, was purified from the culture supernatant of the strain W0101 using ion-exchange chromatography and high-performance liquid chromatography. By analysis of Liquid Chromatograph-Mass Spectrometer, the peptide W1 was identified as a new antifungal peptide derived from the fragment of preprotein translocase subunit YajC. Further analysis revealed that W1 could disrupt the hyphae and spores of Sclerotinia sclerotiorum and inhibit its growth. W1 suppressed S. sclerotiorum and Fusarium oxysporum at a minimum inhibitory concentration of 140 and 58 μg/ml, respectively. The antifungal activity of W1 remained stable at 20–80°C or pH 6–11, with reduced activity at 100–110°C and pH 4–5, and under three protease treatments. Additionally, W1 also had a certain extent of metal ion resistance. These results therefore suggest that the peptide W1 from marine B. amyloliquefaciens W0101 may represent a new antifungal peptide with potential application in the biocontrol of plant diseases. Dataset Arctic Arctic Ocean Frontiers: Figshare Arctic Arctic Ocean |
institution |
Open Polar |
collection |
Frontiers: Figshare |
op_collection_id |
ftfrontimediafig |
language |
unknown |
topic |
Microbiology Microbial Genetics Microbial Ecology Mycology plant pathogenic fungi Bacillus amyloliquefaciens antifungal peptide antifungal activity Arctic Ocean |
spellingShingle |
Microbiology Microbial Genetics Microbial Ecology Mycology plant pathogenic fungi Bacillus amyloliquefaciens antifungal peptide antifungal activity Arctic Ocean Qiao Wen Ruizhe Liu Zhenxiao Ouyang Tianliang He Weini Zhang Xinhua Chen Data_Sheet_1_Identification of a New Antifungal Peptide W1 From a Marine Bacillus amyloliquefaciens Reveals Its Potential in Controlling Fungal Plant Diseases.docx |
topic_facet |
Microbiology Microbial Genetics Microbial Ecology Mycology plant pathogenic fungi Bacillus amyloliquefaciens antifungal peptide antifungal activity Arctic Ocean |
description |
A bacterium, Bacillus amyloliquefaciens W0101, isolated from the Arctic Ocean, showed potent antifungal activity against several plant pathogenic fungi. An antifungal peptide W1, with a molecular weight of approximately 2.4 kDa, was purified from the culture supernatant of the strain W0101 using ion-exchange chromatography and high-performance liquid chromatography. By analysis of Liquid Chromatograph-Mass Spectrometer, the peptide W1 was identified as a new antifungal peptide derived from the fragment of preprotein translocase subunit YajC. Further analysis revealed that W1 could disrupt the hyphae and spores of Sclerotinia sclerotiorum and inhibit its growth. W1 suppressed S. sclerotiorum and Fusarium oxysporum at a minimum inhibitory concentration of 140 and 58 μg/ml, respectively. The antifungal activity of W1 remained stable at 20–80°C or pH 6–11, with reduced activity at 100–110°C and pH 4–5, and under three protease treatments. Additionally, W1 also had a certain extent of metal ion resistance. These results therefore suggest that the peptide W1 from marine B. amyloliquefaciens W0101 may represent a new antifungal peptide with potential application in the biocontrol of plant diseases. |
format |
Dataset |
author |
Qiao Wen Ruizhe Liu Zhenxiao Ouyang Tianliang He Weini Zhang Xinhua Chen |
author_facet |
Qiao Wen Ruizhe Liu Zhenxiao Ouyang Tianliang He Weini Zhang Xinhua Chen |
author_sort |
Qiao Wen |
title |
Data_Sheet_1_Identification of a New Antifungal Peptide W1 From a Marine Bacillus amyloliquefaciens Reveals Its Potential in Controlling Fungal Plant Diseases.docx |
title_short |
Data_Sheet_1_Identification of a New Antifungal Peptide W1 From a Marine Bacillus amyloliquefaciens Reveals Its Potential in Controlling Fungal Plant Diseases.docx |
title_full |
Data_Sheet_1_Identification of a New Antifungal Peptide W1 From a Marine Bacillus amyloliquefaciens Reveals Its Potential in Controlling Fungal Plant Diseases.docx |
title_fullStr |
Data_Sheet_1_Identification of a New Antifungal Peptide W1 From a Marine Bacillus amyloliquefaciens Reveals Its Potential in Controlling Fungal Plant Diseases.docx |
title_full_unstemmed |
Data_Sheet_1_Identification of a New Antifungal Peptide W1 From a Marine Bacillus amyloliquefaciens Reveals Its Potential in Controlling Fungal Plant Diseases.docx |
title_sort |
data_sheet_1_identification of a new antifungal peptide w1 from a marine bacillus amyloliquefaciens reveals its potential in controlling fungal plant diseases.docx |
publishDate |
2022 |
url |
https://doi.org/10.3389/fmicb.2022.922454.s001 https://figshare.com/articles/dataset/Data_Sheet_1_Identification_of_a_New_Antifungal_Peptide_W1_From_a_Marine_Bacillus_amyloliquefaciens_Reveals_Its_Potential_in_Controlling_Fungal_Plant_Diseases_docx/20066435 |
geographic |
Arctic Arctic Ocean |
geographic_facet |
Arctic Arctic Ocean |
genre |
Arctic Arctic Ocean |
genre_facet |
Arctic Arctic Ocean |
op_relation |
doi:10.3389/fmicb.2022.922454.s001 https://figshare.com/articles/dataset/Data_Sheet_1_Identification_of_a_New_Antifungal_Peptide_W1_From_a_Marine_Bacillus_amyloliquefaciens_Reveals_Its_Potential_in_Controlling_Fungal_Plant_Diseases_docx/20066435 |
op_rights |
CC BY 4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fmicb.2022.922454.s001 |
_version_ |
1766330008219942912 |