DataSheet_1_Transcriptome Analysis of Turbot (Scophthalmus maximus) Infected With Aeromonas salmonicida Reveals a Direct Effect on Leptin Synthesis as a Neuroendocrine Mediator of Inflammation and Metabolism Regulation.pdf
Aeromonas salmonicida subsp. salmonicida is the causative agent of furunculosis, a disease affecting numerous fish species worldwide. It is a highly pathogenic bacterium for turbot, whose farming production represents an important economic activity in several European countries and China. To better...
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Online Access: | https://doi.org/10.3389/fmars.2022.888115.s001 https://figshare.com/articles/dataset/DataSheet_1_Transcriptome_Analysis_of_Turbot_Scophthalmus_maximus_Infected_With_Aeromonas_salmonicida_Reveals_a_Direct_Effect_on_Leptin_Synthesis_as_a_Neuroendocrine_Mediator_of_Inflammation_and_Metabolism_Regulation_pdf/20037848 |
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ftfrontimediafig:oai:figshare.com:article/20037848 2023-05-15T18:15:52+02:00 DataSheet_1_Transcriptome Analysis of Turbot (Scophthalmus maximus) Infected With Aeromonas salmonicida Reveals a Direct Effect on Leptin Synthesis as a Neuroendocrine Mediator of Inflammation and Metabolism Regulation.pdf Marta Librán-Pérez Patricia Pereiro Antonio Figueras Beatriz Novoa 2022-06-09T13:13:19Z https://doi.org/10.3389/fmars.2022.888115.s001 https://figshare.com/articles/dataset/DataSheet_1_Transcriptome_Analysis_of_Turbot_Scophthalmus_maximus_Infected_With_Aeromonas_salmonicida_Reveals_a_Direct_Effect_on_Leptin_Synthesis_as_a_Neuroendocrine_Mediator_of_Inflammation_and_Metabolism_Regulation_pdf/20037848 unknown doi:10.3389/fmars.2022.888115.s001 https://figshare.com/articles/dataset/DataSheet_1_Transcriptome_Analysis_of_Turbot_Scophthalmus_maximus_Infected_With_Aeromonas_salmonicida_Reveals_a_Direct_Effect_on_Leptin_Synthesis_as_a_Neuroendocrine_Mediator_of_Inflammation_and_Metabolism_Regulation_pdf/20037848 CC BY 4.0 CC-BY Oceanography Marine Biology Marine Geoscience Biological Oceanography Chemical Oceanography Physical Oceanography Marine Engineering turbot Aeromonas salmonicida RNA-Seq leptin immunometabolism Dataset 2022 ftfrontimediafig https://doi.org/10.3389/fmars.2022.888115.s001 2022-06-15T23:08:35Z Aeromonas salmonicida subsp. salmonicida is the causative agent of furunculosis, a disease affecting numerous fish species worldwide. It is a highly pathogenic bacterium for turbot, whose farming production represents an important economic activity in several European countries and China. To better understand the response of this organism to A. salmonicida, we conducted RNA-Seq analysis of the head kidney from experimentally infected and uninfected turbot juveniles at 24 hours post-infection (hpi). As expected, among the differentially expressed genes (DEGs) between infected and uninfected fish, we observed the modulation of a multitude of immune-related genes but also a high representation of genes linked to metabolism. Interestingly, one of the most upregulated genes was that encoding the hormone leptin. Leptin is a multifunctional hormone/cytokine that has been shown to play roles in the immune system, stress response, food intake, metabolism and energy balance. We used recombinant human leptin to elucidate its role during infection with A. salmonicida in turbot (anorexigenic activity, ability to modulate metabolism and the immune response, and its effect on survival and bacterial load during infection). We found that the intraperitoneal administration of leptin was able to alter the response to the bacteria at the immune level, but especially at the metabolic level, which resulted in a higher survival rate without affecting the bacterial load. Based on this, we hypothesized that leptin could offer great potential as a therapeutic treatment during furunculosis outbreaks by reducing the impact of sepsis. Our results reveal the complex interplay between bacterial activity and the regulation of food intake, metabolism and inflammation. Dataset Scophthalmus maximus Turbot Frontiers: Figshare |
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Open Polar |
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Frontiers: Figshare |
op_collection_id |
ftfrontimediafig |
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unknown |
topic |
Oceanography Marine Biology Marine Geoscience Biological Oceanography Chemical Oceanography Physical Oceanography Marine Engineering turbot Aeromonas salmonicida RNA-Seq leptin immunometabolism |
spellingShingle |
Oceanography Marine Biology Marine Geoscience Biological Oceanography Chemical Oceanography Physical Oceanography Marine Engineering turbot Aeromonas salmonicida RNA-Seq leptin immunometabolism Marta Librán-Pérez Patricia Pereiro Antonio Figueras Beatriz Novoa DataSheet_1_Transcriptome Analysis of Turbot (Scophthalmus maximus) Infected With Aeromonas salmonicida Reveals a Direct Effect on Leptin Synthesis as a Neuroendocrine Mediator of Inflammation and Metabolism Regulation.pdf |
topic_facet |
Oceanography Marine Biology Marine Geoscience Biological Oceanography Chemical Oceanography Physical Oceanography Marine Engineering turbot Aeromonas salmonicida RNA-Seq leptin immunometabolism |
description |
Aeromonas salmonicida subsp. salmonicida is the causative agent of furunculosis, a disease affecting numerous fish species worldwide. It is a highly pathogenic bacterium for turbot, whose farming production represents an important economic activity in several European countries and China. To better understand the response of this organism to A. salmonicida, we conducted RNA-Seq analysis of the head kidney from experimentally infected and uninfected turbot juveniles at 24 hours post-infection (hpi). As expected, among the differentially expressed genes (DEGs) between infected and uninfected fish, we observed the modulation of a multitude of immune-related genes but also a high representation of genes linked to metabolism. Interestingly, one of the most upregulated genes was that encoding the hormone leptin. Leptin is a multifunctional hormone/cytokine that has been shown to play roles in the immune system, stress response, food intake, metabolism and energy balance. We used recombinant human leptin to elucidate its role during infection with A. salmonicida in turbot (anorexigenic activity, ability to modulate metabolism and the immune response, and its effect on survival and bacterial load during infection). We found that the intraperitoneal administration of leptin was able to alter the response to the bacteria at the immune level, but especially at the metabolic level, which resulted in a higher survival rate without affecting the bacterial load. Based on this, we hypothesized that leptin could offer great potential as a therapeutic treatment during furunculosis outbreaks by reducing the impact of sepsis. Our results reveal the complex interplay between bacterial activity and the regulation of food intake, metabolism and inflammation. |
format |
Dataset |
author |
Marta Librán-Pérez Patricia Pereiro Antonio Figueras Beatriz Novoa |
author_facet |
Marta Librán-Pérez Patricia Pereiro Antonio Figueras Beatriz Novoa |
author_sort |
Marta Librán-Pérez |
title |
DataSheet_1_Transcriptome Analysis of Turbot (Scophthalmus maximus) Infected With Aeromonas salmonicida Reveals a Direct Effect on Leptin Synthesis as a Neuroendocrine Mediator of Inflammation and Metabolism Regulation.pdf |
title_short |
DataSheet_1_Transcriptome Analysis of Turbot (Scophthalmus maximus) Infected With Aeromonas salmonicida Reveals a Direct Effect on Leptin Synthesis as a Neuroendocrine Mediator of Inflammation and Metabolism Regulation.pdf |
title_full |
DataSheet_1_Transcriptome Analysis of Turbot (Scophthalmus maximus) Infected With Aeromonas salmonicida Reveals a Direct Effect on Leptin Synthesis as a Neuroendocrine Mediator of Inflammation and Metabolism Regulation.pdf |
title_fullStr |
DataSheet_1_Transcriptome Analysis of Turbot (Scophthalmus maximus) Infected With Aeromonas salmonicida Reveals a Direct Effect on Leptin Synthesis as a Neuroendocrine Mediator of Inflammation and Metabolism Regulation.pdf |
title_full_unstemmed |
DataSheet_1_Transcriptome Analysis of Turbot (Scophthalmus maximus) Infected With Aeromonas salmonicida Reveals a Direct Effect on Leptin Synthesis as a Neuroendocrine Mediator of Inflammation and Metabolism Regulation.pdf |
title_sort |
datasheet_1_transcriptome analysis of turbot (scophthalmus maximus) infected with aeromonas salmonicida reveals a direct effect on leptin synthesis as a neuroendocrine mediator of inflammation and metabolism regulation.pdf |
publishDate |
2022 |
url |
https://doi.org/10.3389/fmars.2022.888115.s001 https://figshare.com/articles/dataset/DataSheet_1_Transcriptome_Analysis_of_Turbot_Scophthalmus_maximus_Infected_With_Aeromonas_salmonicida_Reveals_a_Direct_Effect_on_Leptin_Synthesis_as_a_Neuroendocrine_Mediator_of_Inflammation_and_Metabolism_Regulation_pdf/20037848 |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_relation |
doi:10.3389/fmars.2022.888115.s001 https://figshare.com/articles/dataset/DataSheet_1_Transcriptome_Analysis_of_Turbot_Scophthalmus_maximus_Infected_With_Aeromonas_salmonicida_Reveals_a_Direct_Effect_on_Leptin_Synthesis_as_a_Neuroendocrine_Mediator_of_Inflammation_and_Metabolism_Regulation_pdf/20037848 |
op_rights |
CC BY 4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fmars.2022.888115.s001 |
_version_ |
1766189100150292480 |