Table_6_Synergistic Interaction of Low Salinity Stress With Vibrio Infection Causes Mass Mortalities in the Oyster by Inducing Host Microflora Imbalance and Immune Dysregulation.xlsx
A sudden drop in salinity following extreme precipitation events usually causes mass mortality of oysters exposed to pathogens in ocean environment. While how low salinity stress interacts with pathogens to cause mass mortality remains obscure. In this study, we performed an experiment by low salini...
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Online Access: | https://doi.org/10.3389/fimmu.2022.859975.s008 https://figshare.com/articles/dataset/Table_6_Synergistic_Interaction_of_Low_Salinity_Stress_With_Vibrio_Infection_Causes_Mass_Mortalities_in_the_Oyster_by_Inducing_Host_Microflora_Imbalance_and_Immune_Dysregulation_xlsx/19792384 |
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ftfrontimediafig:oai:figshare.com:article/19792384 2023-05-15T15:58:59+02:00 Table_6_Synergistic Interaction of Low Salinity Stress With Vibrio Infection Causes Mass Mortalities in the Oyster by Inducing Host Microflora Imbalance and Immune Dysregulation.xlsx Xin Li Ben Yang Chenyu Shi Hebing Wang Ruihai Yu Qi Li Shikai Liu 2022-05-19T05:11:26Z https://doi.org/10.3389/fimmu.2022.859975.s008 https://figshare.com/articles/dataset/Table_6_Synergistic_Interaction_of_Low_Salinity_Stress_With_Vibrio_Infection_Causes_Mass_Mortalities_in_the_Oyster_by_Inducing_Host_Microflora_Imbalance_and_Immune_Dysregulation_xlsx/19792384 unknown doi:10.3389/fimmu.2022.859975.s008 https://figshare.com/articles/dataset/Table_6_Synergistic_Interaction_of_Low_Salinity_Stress_With_Vibrio_Infection_Causes_Mass_Mortalities_in_the_Oyster_by_Inducing_Host_Microflora_Imbalance_and_Immune_Dysregulation_xlsx/19792384 CC BY 4.0 CC-BY Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology low salinity stress Crassostrea gigas Vibrio alginolyticus digestive bacterial microbiota immune response Dataset 2022 ftfrontimediafig https://doi.org/10.3389/fimmu.2022.859975.s008 2022-05-25T23:08:35Z A sudden drop in salinity following extreme precipitation events usually causes mass mortality of oysters exposed to pathogens in ocean environment. While how low salinity stress interacts with pathogens to cause mass mortality remains obscure. In this study, we performed an experiment by low salinity stress and pathogen infection with Vibrio alginolyticus to investigate their synergistic effect on the mortality of the Pacific oyster toward understanding of the interaction among environment, host, and pathogen. We showed that low salinity stress did not significantly affect proliferation and virulence of V. alginolyticus, but significantly altered microbial composition and immune response of infected oysters. Microbial community profiling by 16S rRNA amplicon sequencing revealed disrupted homeostasis of digestive bacterial microbiota with the abundance of several pathogenic bacteria being increased, which may affect the pathogenesis in infected oysters. Transcriptome profiling of infected oysters revealed that a large number of genes associated with apoptosis and inflammation were significantly upregulated under low salinity, suggesting that low salinity stress may have triggered immune dysregulation in infected oysters. Our results suggest that host-pathogen interactions are strongly affected by low salinity stress, which is of great significance for assessing future environmental risk of pathogenic diseases, decoding the interaction among environment, host genetics and commensal microbes, and disease surveillance in the oyster. Dataset Crassostrea gigas Pacific oyster Frontiers: Figshare Pacific |
institution |
Open Polar |
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Frontiers: Figshare |
op_collection_id |
ftfrontimediafig |
language |
unknown |
topic |
Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology low salinity stress Crassostrea gigas Vibrio alginolyticus digestive bacterial microbiota immune response |
spellingShingle |
Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology low salinity stress Crassostrea gigas Vibrio alginolyticus digestive bacterial microbiota immune response Xin Li Ben Yang Chenyu Shi Hebing Wang Ruihai Yu Qi Li Shikai Liu Table_6_Synergistic Interaction of Low Salinity Stress With Vibrio Infection Causes Mass Mortalities in the Oyster by Inducing Host Microflora Imbalance and Immune Dysregulation.xlsx |
topic_facet |
Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology low salinity stress Crassostrea gigas Vibrio alginolyticus digestive bacterial microbiota immune response |
description |
A sudden drop in salinity following extreme precipitation events usually causes mass mortality of oysters exposed to pathogens in ocean environment. While how low salinity stress interacts with pathogens to cause mass mortality remains obscure. In this study, we performed an experiment by low salinity stress and pathogen infection with Vibrio alginolyticus to investigate their synergistic effect on the mortality of the Pacific oyster toward understanding of the interaction among environment, host, and pathogen. We showed that low salinity stress did not significantly affect proliferation and virulence of V. alginolyticus, but significantly altered microbial composition and immune response of infected oysters. Microbial community profiling by 16S rRNA amplicon sequencing revealed disrupted homeostasis of digestive bacterial microbiota with the abundance of several pathogenic bacteria being increased, which may affect the pathogenesis in infected oysters. Transcriptome profiling of infected oysters revealed that a large number of genes associated with apoptosis and inflammation were significantly upregulated under low salinity, suggesting that low salinity stress may have triggered immune dysregulation in infected oysters. Our results suggest that host-pathogen interactions are strongly affected by low salinity stress, which is of great significance for assessing future environmental risk of pathogenic diseases, decoding the interaction among environment, host genetics and commensal microbes, and disease surveillance in the oyster. |
format |
Dataset |
author |
Xin Li Ben Yang Chenyu Shi Hebing Wang Ruihai Yu Qi Li Shikai Liu |
author_facet |
Xin Li Ben Yang Chenyu Shi Hebing Wang Ruihai Yu Qi Li Shikai Liu |
author_sort |
Xin Li |
title |
Table_6_Synergistic Interaction of Low Salinity Stress With Vibrio Infection Causes Mass Mortalities in the Oyster by Inducing Host Microflora Imbalance and Immune Dysregulation.xlsx |
title_short |
Table_6_Synergistic Interaction of Low Salinity Stress With Vibrio Infection Causes Mass Mortalities in the Oyster by Inducing Host Microflora Imbalance and Immune Dysregulation.xlsx |
title_full |
Table_6_Synergistic Interaction of Low Salinity Stress With Vibrio Infection Causes Mass Mortalities in the Oyster by Inducing Host Microflora Imbalance and Immune Dysregulation.xlsx |
title_fullStr |
Table_6_Synergistic Interaction of Low Salinity Stress With Vibrio Infection Causes Mass Mortalities in the Oyster by Inducing Host Microflora Imbalance and Immune Dysregulation.xlsx |
title_full_unstemmed |
Table_6_Synergistic Interaction of Low Salinity Stress With Vibrio Infection Causes Mass Mortalities in the Oyster by Inducing Host Microflora Imbalance and Immune Dysregulation.xlsx |
title_sort |
table_6_synergistic interaction of low salinity stress with vibrio infection causes mass mortalities in the oyster by inducing host microflora imbalance and immune dysregulation.xlsx |
publishDate |
2022 |
url |
https://doi.org/10.3389/fimmu.2022.859975.s008 https://figshare.com/articles/dataset/Table_6_Synergistic_Interaction_of_Low_Salinity_Stress_With_Vibrio_Infection_Causes_Mass_Mortalities_in_the_Oyster_by_Inducing_Host_Microflora_Imbalance_and_Immune_Dysregulation_xlsx/19792384 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_relation |
doi:10.3389/fimmu.2022.859975.s008 https://figshare.com/articles/dataset/Table_6_Synergistic_Interaction_of_Low_Salinity_Stress_With_Vibrio_Infection_Causes_Mass_Mortalities_in_the_Oyster_by_Inducing_Host_Microflora_Imbalance_and_Immune_Dysregulation_xlsx/19792384 |
op_rights |
CC BY 4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fimmu.2022.859975.s008 |
_version_ |
1766394772701839360 |