Table_1_Genomic Sequencing and Analysis of a Novel Human Cowpox Virus With Mosaic Sequences From North America and Old World Orthopoxvirus.XLSX

• Main Authors: Diana Diaz-Cánova, Ugo L. Moens, Annika Brinkmann, Andreas Nitsche, Malachy Ifeanyi Okeke
• Format: Dataset
• Language: unknown
• Published: 2022
• Subjects: Microbiology; Microbial Genetics; Microbial Ecology; Mycology; poxvirus; phylogenetics; Fennoscandian; Norway; recombination; Northern Norway
• Online Access: https://doi.org/10.3389/fmicb.2022.868887.s002; https://figshare.com/articles/dataset/Table_1_Genomic_Sequencing_and_Analysis_of_a_Novel_Human_Cowpox_Virus_With_Mosaic_Sequences_From_North_America_and_Old_World_Orthopoxvirus_XLSX/19698478

Orthopoxviruses (OPXVs) not only infect their natural hosts, but some OPXVs can also cause disease in humans. Previously, we partially characterized an OPXV isolated from an 18-year-old male living in Northern Norway. Restriction enzyme analysis and partial genome sequencing characterized this virus as an atypical cowpox virus (CPXV), which we named CPXV-No-H2. In this study, we determined the complete genome sequence of CPXV-No-H2 using Illumina and Nanopore sequencing. Our results showed that the whole CPXV-No-H2 genome is 220,276 base pairs (bp) in length, with inverted terminal repeat regions of approximately 7 kbp, containing 217 predicted genes. Seventeen predicted CPXV-No-H2 proteins were most similar to OPXV proteins from the Old World, including Ectromelia virus (ECTV) and Vaccinia virus, and North America, Alaskapox virus (AKPV). CPXV-No-H2 has a mosaic genome with genes most similar to other OPXV genes, and seven potential recombination events were identified. The phylogenetic analysis showed that CPXV-No-H2 formed a separate clade with the German CPXV isolates CPXV_GerMygEK938_17 and CPXV_Ger2010_MKY, sharing 96.4 and 96.3% nucleotide identity, respectively, and this clade clustered closely with the ECTV-OPXV Abatino clade. CPXV-No-H2 is a mosaic virus that may have arisen out of several recombination events between OPXVs, and its phylogenetic clustering suggests that ECTV-Abatino-like cowpox viruses form a distinct, new clade of cowpox viruses.