Image_1_Identification of Antibacterial Activity of Hepcidin From Antarctic Notothenioid Fish.tif
Hepcidin is a small peptide composed of signal peptide, propeptide, and the bioactive mature peptide from N terminal to C terminal. Mature hepcidin is an antibacterial peptide and iron regulator with eight highly conserved cysteines forming four intramolecular disulfide bonds, giving it a β sheet ha...
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ftfrontimediafig:oai:figshare.com:article/19581469 2023-05-15T13:42:18+02:00 Image_1_Identification of Antibacterial Activity of Hepcidin From Antarctic Notothenioid Fish.tif Mingli Liu Ruiqin Hu Wenhao Li Wenyi Yang Qianghua Xu Liangbiao Chen 2022-04-12T05:19:49Z https://doi.org/10.3389/fmicb.2022.834477.s001 https://figshare.com/articles/figure/Image_1_Identification_of_Antibacterial_Activity_of_Hepcidin_From_Antarctic_Notothenioid_Fish_tif/19581469 unknown doi:10.3389/fmicb.2022.834477.s001 https://figshare.com/articles/figure/Image_1_Identification_of_Antibacterial_Activity_of_Hepcidin_From_Antarctic_Notothenioid_Fish_tif/19581469 CC BY 4.0 CC-BY Microbiology Microbial Genetics Microbial Ecology Mycology hepcidin Antarctic notothenioid fish antibacterial peptide recombinant protein antibacterial activity Image Figure 2022 ftfrontimediafig https://doi.org/10.3389/fmicb.2022.834477.s001 2022-04-13T23:04:33Z Hepcidin is a small peptide composed of signal peptide, propeptide, and the bioactive mature peptide from N terminal to C terminal. Mature hepcidin is an antibacterial peptide and iron regulator with eight highly conserved cysteines forming four intramolecular disulfide bonds, giving it a β sheet hairpin-like structure. Hepcidin homologs are found in a variety of vertebrates, especially fish, and their diversity may be associated with different habitats and different levels of pathogens. Dissostichus mawsoni, an Antarctic notothenioid fish that lives in the coldest water unlike most places of the world, with at least two hepcidin variants with eight cysteines. We confirmed the formation process of activated mature hepcidins from D. mawsoni in Chinese hamster ovary (CHO) cell line, obtained recombinant hepcidin protein from prokaryotes, and characterized its binding ability and antibacterial activity against varying bacteria. The expression of hepcidin in CHO cell line showed that the prepropeptide of Dmhep_8cysV1 and Dmhep_8cysV2 cleavage into smaller mature peptide. The antibacterial assay and flow cytometry showed that Dmhep_8cysV1, Dmhep_8cysV2, and Drhep bound to different bacteria and killed them with different minimum inhibitory concentration. These data suggest that hepcidin plays an important role in the innate immunity of D. mawsoni and is of great value in improving resistance to pathogens. Still Image Antarc* Antarctic Frontiers: Figshare Antarctic |
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Frontiers: Figshare |
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ftfrontimediafig |
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unknown |
topic |
Microbiology Microbial Genetics Microbial Ecology Mycology hepcidin Antarctic notothenioid fish antibacterial peptide recombinant protein antibacterial activity |
spellingShingle |
Microbiology Microbial Genetics Microbial Ecology Mycology hepcidin Antarctic notothenioid fish antibacterial peptide recombinant protein antibacterial activity Mingli Liu Ruiqin Hu Wenhao Li Wenyi Yang Qianghua Xu Liangbiao Chen Image_1_Identification of Antibacterial Activity of Hepcidin From Antarctic Notothenioid Fish.tif |
topic_facet |
Microbiology Microbial Genetics Microbial Ecology Mycology hepcidin Antarctic notothenioid fish antibacterial peptide recombinant protein antibacterial activity |
description |
Hepcidin is a small peptide composed of signal peptide, propeptide, and the bioactive mature peptide from N terminal to C terminal. Mature hepcidin is an antibacterial peptide and iron regulator with eight highly conserved cysteines forming four intramolecular disulfide bonds, giving it a β sheet hairpin-like structure. Hepcidin homologs are found in a variety of vertebrates, especially fish, and their diversity may be associated with different habitats and different levels of pathogens. Dissostichus mawsoni, an Antarctic notothenioid fish that lives in the coldest water unlike most places of the world, with at least two hepcidin variants with eight cysteines. We confirmed the formation process of activated mature hepcidins from D. mawsoni in Chinese hamster ovary (CHO) cell line, obtained recombinant hepcidin protein from prokaryotes, and characterized its binding ability and antibacterial activity against varying bacteria. The expression of hepcidin in CHO cell line showed that the prepropeptide of Dmhep_8cysV1 and Dmhep_8cysV2 cleavage into smaller mature peptide. The antibacterial assay and flow cytometry showed that Dmhep_8cysV1, Dmhep_8cysV2, and Drhep bound to different bacteria and killed them with different minimum inhibitory concentration. These data suggest that hepcidin plays an important role in the innate immunity of D. mawsoni and is of great value in improving resistance to pathogens. |
format |
Still Image |
author |
Mingli Liu Ruiqin Hu Wenhao Li Wenyi Yang Qianghua Xu Liangbiao Chen |
author_facet |
Mingli Liu Ruiqin Hu Wenhao Li Wenyi Yang Qianghua Xu Liangbiao Chen |
author_sort |
Mingli Liu |
title |
Image_1_Identification of Antibacterial Activity of Hepcidin From Antarctic Notothenioid Fish.tif |
title_short |
Image_1_Identification of Antibacterial Activity of Hepcidin From Antarctic Notothenioid Fish.tif |
title_full |
Image_1_Identification of Antibacterial Activity of Hepcidin From Antarctic Notothenioid Fish.tif |
title_fullStr |
Image_1_Identification of Antibacterial Activity of Hepcidin From Antarctic Notothenioid Fish.tif |
title_full_unstemmed |
Image_1_Identification of Antibacterial Activity of Hepcidin From Antarctic Notothenioid Fish.tif |
title_sort |
image_1_identification of antibacterial activity of hepcidin from antarctic notothenioid fish.tif |
publishDate |
2022 |
url |
https://doi.org/10.3389/fmicb.2022.834477.s001 https://figshare.com/articles/figure/Image_1_Identification_of_Antibacterial_Activity_of_Hepcidin_From_Antarctic_Notothenioid_Fish_tif/19581469 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_relation |
doi:10.3389/fmicb.2022.834477.s001 https://figshare.com/articles/figure/Image_1_Identification_of_Antibacterial_Activity_of_Hepcidin_From_Antarctic_Notothenioid_Fish_tif/19581469 |
op_rights |
CC BY 4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fmicb.2022.834477.s001 |
_version_ |
1766166249964830720 |