Image_1_Immunocytochemical Localization of Enzymes Involved in Dopamine, Serotonin, and Acetylcholine Synthesis in the Optic Neuropils and Neuroendocrine System of Eyestalks of Paralithodes camtschaticus.JPEG
Identifying the neurotransmitters secreted by specific neurons in crustacean eyestalks is crucial to understanding their physiological roles. Here, we combined immunocytochemistry with confocal microscopy and identified the neurotransmitters dopamine (DA), serotonin (5-HT), and acetylcholine (ACh) i...
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ftfrontimediafig:oai:figshare.com:article/19547167 2023-05-15T17:54:37+02:00 Image_1_Immunocytochemical Localization of Enzymes Involved in Dopamine, Serotonin, and Acetylcholine Synthesis in the Optic Neuropils and Neuroendocrine System of Eyestalks of Paralithodes camtschaticus.JPEG Elena Kotsyuba Vyacheslav Dyachuk 2022-04-08T04:54:00Z https://doi.org/10.3389/fnana.2022.844654.s001 https://figshare.com/articles/figure/Image_1_Immunocytochemical_Localization_of_Enzymes_Involved_in_Dopamine_Serotonin_and_Acetylcholine_Synthesis_in_the_Optic_Neuropils_and_Neuroendocrine_System_of_Eyestalks_of_Paralithodes_camtschaticus_JPEG/19547167 unknown doi:10.3389/fnana.2022.844654.s001 https://figshare.com/articles/figure/Image_1_Immunocytochemical_Localization_of_Enzymes_Involved_in_Dopamine_Serotonin_and_Acetylcholine_Synthesis_in_the_Optic_Neuropils_and_Neuroendocrine_System_of_Eyestalks_of_Paralithodes_camtschaticus_JPEG/19547167 CC BY 4.0 CC-BY Anatomy Neuroscience Cellular Nervous System Central Nervous System serotonin acetylcholine tyrosine hydroxylase sinus gland dopamine king crab crustacea Image Figure 2022 ftfrontimediafig https://doi.org/10.3389/fnana.2022.844654.s001 2022-04-13T23:08:41Z Identifying the neurotransmitters secreted by specific neurons in crustacean eyestalks is crucial to understanding their physiological roles. Here, we combined immunocytochemistry with confocal microscopy and identified the neurotransmitters dopamine (DA), serotonin (5-HT), and acetylcholine (ACh) in the optic neuropils and X-organ sinus gland (XO-SG) complex of the eyestalks of Paralithodes camtschaticus (red king crab). The distribution of Ach neurons was studied by choline acetyltransferase (ChAT) immunohistochemistry and compared with that of DA neurons examined in the same or adjacent sections by tyrosine hydroxylase (TH) immunohistochemistry. We detected 5-HT, TH, and ChAT in columnar, amacrine, and tangential neurons in the optic neuropils and established the presence of immunoreactive fibers and neurons in the terminal medulla in the XO region of the lateral protocerebrum. Additionally, we detected ChAT and 5-HT in the endogenous cells of the SG of P. camtschaticus for the first time. Furthermore, localization of 5-HT- and ChAT-positive cells in the SG indicated that these neurotransmitters locally modulate the secretion of neurohormones that are synthesized in the XO. These findings establish the presence of several neurotransmitters in the XO-SG complex of P. camtschaticus. Still Image Paralithodes camtschaticus Red king crab Frontiers: Figshare |
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Frontiers: Figshare |
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Anatomy Neuroscience Cellular Nervous System Central Nervous System serotonin acetylcholine tyrosine hydroxylase sinus gland dopamine king crab crustacea |
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Anatomy Neuroscience Cellular Nervous System Central Nervous System serotonin acetylcholine tyrosine hydroxylase sinus gland dopamine king crab crustacea Elena Kotsyuba Vyacheslav Dyachuk Image_1_Immunocytochemical Localization of Enzymes Involved in Dopamine, Serotonin, and Acetylcholine Synthesis in the Optic Neuropils and Neuroendocrine System of Eyestalks of Paralithodes camtschaticus.JPEG |
topic_facet |
Anatomy Neuroscience Cellular Nervous System Central Nervous System serotonin acetylcholine tyrosine hydroxylase sinus gland dopamine king crab crustacea |
description |
Identifying the neurotransmitters secreted by specific neurons in crustacean eyestalks is crucial to understanding their physiological roles. Here, we combined immunocytochemistry with confocal microscopy and identified the neurotransmitters dopamine (DA), serotonin (5-HT), and acetylcholine (ACh) in the optic neuropils and X-organ sinus gland (XO-SG) complex of the eyestalks of Paralithodes camtschaticus (red king crab). The distribution of Ach neurons was studied by choline acetyltransferase (ChAT) immunohistochemistry and compared with that of DA neurons examined in the same or adjacent sections by tyrosine hydroxylase (TH) immunohistochemistry. We detected 5-HT, TH, and ChAT in columnar, amacrine, and tangential neurons in the optic neuropils and established the presence of immunoreactive fibers and neurons in the terminal medulla in the XO region of the lateral protocerebrum. Additionally, we detected ChAT and 5-HT in the endogenous cells of the SG of P. camtschaticus for the first time. Furthermore, localization of 5-HT- and ChAT-positive cells in the SG indicated that these neurotransmitters locally modulate the secretion of neurohormones that are synthesized in the XO. These findings establish the presence of several neurotransmitters in the XO-SG complex of P. camtschaticus. |
format |
Still Image |
author |
Elena Kotsyuba Vyacheslav Dyachuk |
author_facet |
Elena Kotsyuba Vyacheslav Dyachuk |
author_sort |
Elena Kotsyuba |
title |
Image_1_Immunocytochemical Localization of Enzymes Involved in Dopamine, Serotonin, and Acetylcholine Synthesis in the Optic Neuropils and Neuroendocrine System of Eyestalks of Paralithodes camtschaticus.JPEG |
title_short |
Image_1_Immunocytochemical Localization of Enzymes Involved in Dopamine, Serotonin, and Acetylcholine Synthesis in the Optic Neuropils and Neuroendocrine System of Eyestalks of Paralithodes camtschaticus.JPEG |
title_full |
Image_1_Immunocytochemical Localization of Enzymes Involved in Dopamine, Serotonin, and Acetylcholine Synthesis in the Optic Neuropils and Neuroendocrine System of Eyestalks of Paralithodes camtschaticus.JPEG |
title_fullStr |
Image_1_Immunocytochemical Localization of Enzymes Involved in Dopamine, Serotonin, and Acetylcholine Synthesis in the Optic Neuropils and Neuroendocrine System of Eyestalks of Paralithodes camtschaticus.JPEG |
title_full_unstemmed |
Image_1_Immunocytochemical Localization of Enzymes Involved in Dopamine, Serotonin, and Acetylcholine Synthesis in the Optic Neuropils and Neuroendocrine System of Eyestalks of Paralithodes camtschaticus.JPEG |
title_sort |
image_1_immunocytochemical localization of enzymes involved in dopamine, serotonin, and acetylcholine synthesis in the optic neuropils and neuroendocrine system of eyestalks of paralithodes camtschaticus.jpeg |
publishDate |
2022 |
url |
https://doi.org/10.3389/fnana.2022.844654.s001 https://figshare.com/articles/figure/Image_1_Immunocytochemical_Localization_of_Enzymes_Involved_in_Dopamine_Serotonin_and_Acetylcholine_Synthesis_in_the_Optic_Neuropils_and_Neuroendocrine_System_of_Eyestalks_of_Paralithodes_camtschaticus_JPEG/19547167 |
genre |
Paralithodes camtschaticus Red king crab |
genre_facet |
Paralithodes camtschaticus Red king crab |
op_relation |
doi:10.3389/fnana.2022.844654.s001 https://figshare.com/articles/figure/Image_1_Immunocytochemical_Localization_of_Enzymes_Involved_in_Dopamine_Serotonin_and_Acetylcholine_Synthesis_in_the_Optic_Neuropils_and_Neuroendocrine_System_of_Eyestalks_of_Paralithodes_camtschaticus_JPEG/19547167 |
op_rights |
CC BY 4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fnana.2022.844654.s001 |
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1766162418519506944 |