Table_2_Reactive Oxygen Species Partly Mediate DNA Methylation in Responses to Different Heavy Metals in Pokeweed.XLSX
DNA methylation is a rapid response strategy promoting plant survival under heavy metal (HM) stress. However, the roles of DNA methylation underlying plant adaptation to HM stress remain largely unknown. Here, we used pokeweed, a hyperaccumulator of manganese (Mn) and cadmium (Cd), to explore respon...
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ftfrontimediafig:oai:figshare.com:article/19531930 2023-05-15T16:01:45+02:00 Table_2_Reactive Oxygen Species Partly Mediate DNA Methylation in Responses to Different Heavy Metals in Pokeweed.XLSX Minyu Jing Hanchao Zhang Mingyue Wei Yongwei Tang Yan Xia Yahua Chen Zhenguo Shen Chen Chen 2022-04-07T05:03:12Z https://doi.org/10.3389/fpls.2022.845108.s002 https://figshare.com/articles/dataset/Table_2_Reactive_Oxygen_Species_Partly_Mediate_DNA_Methylation_in_Responses_to_Different_Heavy_Metals_in_Pokeweed_XLSX/19531930 unknown doi:10.3389/fpls.2022.845108.s002 https://figshare.com/articles/dataset/Table_2_Reactive_Oxygen_Species_Partly_Mediate_DNA_Methylation_in_Responses_to_Different_Heavy_Metals_in_Pokeweed_XLSX/19531930 CC BY 4.0 CC-BY Botany Plant Biology Plant Systematics and Taxonomy Plant Cell and Molecular Biology Plant Developmental and Reproductive Biology Plant Pathology Plant Physiology Plant Biology not elsewhere classified heavy metal pokeweed reactive oxygen species DNA methylation methylase and demethylase Dataset 2022 ftfrontimediafig https://doi.org/10.3389/fpls.2022.845108.s002 2022-04-13T23:10:14Z DNA methylation is a rapid response strategy promoting plant survival under heavy metal (HM) stress. However, the roles of DNA methylation underlying plant adaptation to HM stress remain largely unknown. Here, we used pokeweed, a hyperaccumulator of manganese (Mn) and cadmium (Cd), to explore responses of plant to HM stress at phenotypic, transcriptional and DNA methylation levels. Mn- and Cd-specific response patterns were detected in pokeweed. The growth of pokeweed was both inhibited with exposure to excess Mn/Cd, but pokeweed distinguished Mn and Cd with different subcellular distributions, ROS scavenging systems, transcriptional patterns including genes involved in DNA methylation, and differentially methylated loci (DML). The number of DML between Mn/Cd treated and untreated samples increased with increased Mn/Cd concentrations. Meanwhile, pretreatment with NADPH oxidase inhibitors prior to HM exposure markedly reduced HM-induced reactive oxygen species (ROS), which caused reductions in expressions of DNA methylase and demethylase in pretreated samples. The increased levels of HM-induced demethylation were suppressed with alleviated ROS stress, and a series of HM-related methylated loci were also ROS-related. Taken together, our study demonstrates that different HMs affect different DNA methylation sites in a dose-dependent manner and changes in DNA methylation under Mn/Cd stress are partly mediated by HM-induced ROS. Dataset DML Frontiers: Figshare |
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Frontiers: Figshare |
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ftfrontimediafig |
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Botany Plant Biology Plant Systematics and Taxonomy Plant Cell and Molecular Biology Plant Developmental and Reproductive Biology Plant Pathology Plant Physiology Plant Biology not elsewhere classified heavy metal pokeweed reactive oxygen species DNA methylation methylase and demethylase |
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Botany Plant Biology Plant Systematics and Taxonomy Plant Cell and Molecular Biology Plant Developmental and Reproductive Biology Plant Pathology Plant Physiology Plant Biology not elsewhere classified heavy metal pokeweed reactive oxygen species DNA methylation methylase and demethylase Minyu Jing Hanchao Zhang Mingyue Wei Yongwei Tang Yan Xia Yahua Chen Zhenguo Shen Chen Chen Table_2_Reactive Oxygen Species Partly Mediate DNA Methylation in Responses to Different Heavy Metals in Pokeweed.XLSX |
topic_facet |
Botany Plant Biology Plant Systematics and Taxonomy Plant Cell and Molecular Biology Plant Developmental and Reproductive Biology Plant Pathology Plant Physiology Plant Biology not elsewhere classified heavy metal pokeweed reactive oxygen species DNA methylation methylase and demethylase |
description |
DNA methylation is a rapid response strategy promoting plant survival under heavy metal (HM) stress. However, the roles of DNA methylation underlying plant adaptation to HM stress remain largely unknown. Here, we used pokeweed, a hyperaccumulator of manganese (Mn) and cadmium (Cd), to explore responses of plant to HM stress at phenotypic, transcriptional and DNA methylation levels. Mn- and Cd-specific response patterns were detected in pokeweed. The growth of pokeweed was both inhibited with exposure to excess Mn/Cd, but pokeweed distinguished Mn and Cd with different subcellular distributions, ROS scavenging systems, transcriptional patterns including genes involved in DNA methylation, and differentially methylated loci (DML). The number of DML between Mn/Cd treated and untreated samples increased with increased Mn/Cd concentrations. Meanwhile, pretreatment with NADPH oxidase inhibitors prior to HM exposure markedly reduced HM-induced reactive oxygen species (ROS), which caused reductions in expressions of DNA methylase and demethylase in pretreated samples. The increased levels of HM-induced demethylation were suppressed with alleviated ROS stress, and a series of HM-related methylated loci were also ROS-related. Taken together, our study demonstrates that different HMs affect different DNA methylation sites in a dose-dependent manner and changes in DNA methylation under Mn/Cd stress are partly mediated by HM-induced ROS. |
format |
Dataset |
author |
Minyu Jing Hanchao Zhang Mingyue Wei Yongwei Tang Yan Xia Yahua Chen Zhenguo Shen Chen Chen |
author_facet |
Minyu Jing Hanchao Zhang Mingyue Wei Yongwei Tang Yan Xia Yahua Chen Zhenguo Shen Chen Chen |
author_sort |
Minyu Jing |
title |
Table_2_Reactive Oxygen Species Partly Mediate DNA Methylation in Responses to Different Heavy Metals in Pokeweed.XLSX |
title_short |
Table_2_Reactive Oxygen Species Partly Mediate DNA Methylation in Responses to Different Heavy Metals in Pokeweed.XLSX |
title_full |
Table_2_Reactive Oxygen Species Partly Mediate DNA Methylation in Responses to Different Heavy Metals in Pokeweed.XLSX |
title_fullStr |
Table_2_Reactive Oxygen Species Partly Mediate DNA Methylation in Responses to Different Heavy Metals in Pokeweed.XLSX |
title_full_unstemmed |
Table_2_Reactive Oxygen Species Partly Mediate DNA Methylation in Responses to Different Heavy Metals in Pokeweed.XLSX |
title_sort |
table_2_reactive oxygen species partly mediate dna methylation in responses to different heavy metals in pokeweed.xlsx |
publishDate |
2022 |
url |
https://doi.org/10.3389/fpls.2022.845108.s002 https://figshare.com/articles/dataset/Table_2_Reactive_Oxygen_Species_Partly_Mediate_DNA_Methylation_in_Responses_to_Different_Heavy_Metals_in_Pokeweed_XLSX/19531930 |
genre |
DML |
genre_facet |
DML |
op_relation |
doi:10.3389/fpls.2022.845108.s002 https://figshare.com/articles/dataset/Table_2_Reactive_Oxygen_Species_Partly_Mediate_DNA_Methylation_in_Responses_to_Different_Heavy_Metals_in_Pokeweed_XLSX/19531930 |
op_rights |
CC BY 4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fpls.2022.845108.s002 |
_version_ |
1766397492471005184 |