Table_4_Interacting Effects of Sea Louse (Lepeophtheirus salmonis) Infection and Formalin-Killed Aeromonas salmonicida on Atlantic Salmon Skin Transcriptome.xlsx
Lepeophtheirus salmonis (sea lice) and bacterial co-infection threatens wild and farmed Atlantic salmon performance and welfare. In the present study, pre-adult L. salmonis-infected and non-infected salmon were intraperitoneally injected with either formalin-killed Aeromonas salmonicida bacterin (AS...
Main Authors: | , , , , , , , , , , |
---|---|
Format: | Dataset |
Language: | unknown |
Published: |
2022
|
Subjects: | |
Online Access: | https://doi.org/10.3389/fimmu.2022.804987.s007 https://figshare.com/articles/dataset/Table_4_Interacting_Effects_of_Sea_Louse_Lepeophtheirus_salmonis_Infection_and_Formalin-Killed_Aeromonas_salmonicida_on_Atlantic_Salmon_Skin_Transcriptome_xlsx/19407848 |
id |
ftfrontimediafig:oai:figshare.com:article/19407848 |
---|---|
record_format |
openpolar |
spelling |
ftfrontimediafig:oai:figshare.com:article/19407848 2023-05-15T15:31:27+02:00 Table_4_Interacting Effects of Sea Louse (Lepeophtheirus salmonis) Infection and Formalin-Killed Aeromonas salmonicida on Atlantic Salmon Skin Transcriptome.xlsx Albert Caballero-Solares Navaneethaiyer Umasuthan Xi Xue Tomer Katan Surendra Kumar Jillian D. Westcott Zhiyu Chen Mark D. Fast Stanko Skugor Richard G. Taylor Matthew L. Rise 2022-03-24T04:19:59Z https://doi.org/10.3389/fimmu.2022.804987.s007 https://figshare.com/articles/dataset/Table_4_Interacting_Effects_of_Sea_Louse_Lepeophtheirus_salmonis_Infection_and_Formalin-Killed_Aeromonas_salmonicida_on_Atlantic_Salmon_Skin_Transcriptome_xlsx/19407848 unknown doi:10.3389/fimmu.2022.804987.s007 https://figshare.com/articles/dataset/Table_4_Interacting_Effects_of_Sea_Louse_Lepeophtheirus_salmonis_Infection_and_Formalin-Killed_Aeromonas_salmonicida_on_Atlantic_Salmon_Skin_Transcriptome_xlsx/19407848 CC BY 4.0 CC-BY Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology Atlantic salmon sea lice formalin-killed bacterin Aeromonas salmonicida skin transcriptome Dataset 2022 ftfrontimediafig https://doi.org/10.3389/fimmu.2022.804987.s007 2022-03-30T23:09:34Z Lepeophtheirus salmonis (sea lice) and bacterial co-infection threatens wild and farmed Atlantic salmon performance and welfare. In the present study, pre-adult L. salmonis-infected and non-infected salmon were intraperitoneally injected with either formalin-killed Aeromonas salmonicida bacterin (ASAL) or phosphate-buffered saline (PBS). Dorsal skin samples from each injection/infection group (PBS/no lice, PBS/lice, ASAL/no lice, and ASAL/lice) were collected at 24 h post-injection and used for transcriptome profiling using a 44K salmonid microarray platform. Microarray results showed no clear inflammation gene expression signatures and revealed extensive gene repression effects by pre-adult lice (2,189 down and 345 up-regulated probes) in the PBS-injected salmon (PBS/lice vs. PBS/no lice), which involved basic cellular (e.g., RNA and protein metabolism) processes. Lice repressive effects were not observed within the group of ASAL-injected salmon (ASAL/lice vs. ASAL/no lice); on the contrary, the observed skin transcriptome changes –albeit of lesser magnitude (82 up and 1 down-regulated probes)– suggested the activation in key immune and wound healing processes (e.g., neutrophil degranulation, keratinocyte differentiation). The molecular skin response to ASAL was more intense in the lice-infected (ASAL/lice vs. PBS/lice; 272 up and 11 down-regulated probes) than in the non-infected fish (ASAL/no lice vs. PBS/no lice; 27 up-regulated probes). Regardless of lice infection, the skin’s response to ASAL was characterized by the putative activation of both antibacterial and wound healing pathways. The transcriptomic changes prompted by ASAL+lice co-stimulation (ASAL/lice vs. PBS/no lice; 1878 up and 3120 down-regulated probes) confirmed partial mitigation of lice repressive effects on fundamental cellular processes and the activation of pathways involved in innate (e.g., neutrophil degranulation) and adaptive immunity (e.g., antibody formation), as well as endothelial cell migration. The qPCR analyses evidenced ... Dataset Atlantic salmon Frontiers: Figshare |
institution |
Open Polar |
collection |
Frontiers: Figshare |
op_collection_id |
ftfrontimediafig |
language |
unknown |
topic |
Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology Atlantic salmon sea lice formalin-killed bacterin Aeromonas salmonicida skin transcriptome |
spellingShingle |
Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology Atlantic salmon sea lice formalin-killed bacterin Aeromonas salmonicida skin transcriptome Albert Caballero-Solares Navaneethaiyer Umasuthan Xi Xue Tomer Katan Surendra Kumar Jillian D. Westcott Zhiyu Chen Mark D. Fast Stanko Skugor Richard G. Taylor Matthew L. Rise Table_4_Interacting Effects of Sea Louse (Lepeophtheirus salmonis) Infection and Formalin-Killed Aeromonas salmonicida on Atlantic Salmon Skin Transcriptome.xlsx |
topic_facet |
Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology Atlantic salmon sea lice formalin-killed bacterin Aeromonas salmonicida skin transcriptome |
description |
Lepeophtheirus salmonis (sea lice) and bacterial co-infection threatens wild and farmed Atlantic salmon performance and welfare. In the present study, pre-adult L. salmonis-infected and non-infected salmon were intraperitoneally injected with either formalin-killed Aeromonas salmonicida bacterin (ASAL) or phosphate-buffered saline (PBS). Dorsal skin samples from each injection/infection group (PBS/no lice, PBS/lice, ASAL/no lice, and ASAL/lice) were collected at 24 h post-injection and used for transcriptome profiling using a 44K salmonid microarray platform. Microarray results showed no clear inflammation gene expression signatures and revealed extensive gene repression effects by pre-adult lice (2,189 down and 345 up-regulated probes) in the PBS-injected salmon (PBS/lice vs. PBS/no lice), which involved basic cellular (e.g., RNA and protein metabolism) processes. Lice repressive effects were not observed within the group of ASAL-injected salmon (ASAL/lice vs. ASAL/no lice); on the contrary, the observed skin transcriptome changes –albeit of lesser magnitude (82 up and 1 down-regulated probes)– suggested the activation in key immune and wound healing processes (e.g., neutrophil degranulation, keratinocyte differentiation). The molecular skin response to ASAL was more intense in the lice-infected (ASAL/lice vs. PBS/lice; 272 up and 11 down-regulated probes) than in the non-infected fish (ASAL/no lice vs. PBS/no lice; 27 up-regulated probes). Regardless of lice infection, the skin’s response to ASAL was characterized by the putative activation of both antibacterial and wound healing pathways. The transcriptomic changes prompted by ASAL+lice co-stimulation (ASAL/lice vs. PBS/no lice; 1878 up and 3120 down-regulated probes) confirmed partial mitigation of lice repressive effects on fundamental cellular processes and the activation of pathways involved in innate (e.g., neutrophil degranulation) and adaptive immunity (e.g., antibody formation), as well as endothelial cell migration. The qPCR analyses evidenced ... |
format |
Dataset |
author |
Albert Caballero-Solares Navaneethaiyer Umasuthan Xi Xue Tomer Katan Surendra Kumar Jillian D. Westcott Zhiyu Chen Mark D. Fast Stanko Skugor Richard G. Taylor Matthew L. Rise |
author_facet |
Albert Caballero-Solares Navaneethaiyer Umasuthan Xi Xue Tomer Katan Surendra Kumar Jillian D. Westcott Zhiyu Chen Mark D. Fast Stanko Skugor Richard G. Taylor Matthew L. Rise |
author_sort |
Albert Caballero-Solares |
title |
Table_4_Interacting Effects of Sea Louse (Lepeophtheirus salmonis) Infection and Formalin-Killed Aeromonas salmonicida on Atlantic Salmon Skin Transcriptome.xlsx |
title_short |
Table_4_Interacting Effects of Sea Louse (Lepeophtheirus salmonis) Infection and Formalin-Killed Aeromonas salmonicida on Atlantic Salmon Skin Transcriptome.xlsx |
title_full |
Table_4_Interacting Effects of Sea Louse (Lepeophtheirus salmonis) Infection and Formalin-Killed Aeromonas salmonicida on Atlantic Salmon Skin Transcriptome.xlsx |
title_fullStr |
Table_4_Interacting Effects of Sea Louse (Lepeophtheirus salmonis) Infection and Formalin-Killed Aeromonas salmonicida on Atlantic Salmon Skin Transcriptome.xlsx |
title_full_unstemmed |
Table_4_Interacting Effects of Sea Louse (Lepeophtheirus salmonis) Infection and Formalin-Killed Aeromonas salmonicida on Atlantic Salmon Skin Transcriptome.xlsx |
title_sort |
table_4_interacting effects of sea louse (lepeophtheirus salmonis) infection and formalin-killed aeromonas salmonicida on atlantic salmon skin transcriptome.xlsx |
publishDate |
2022 |
url |
https://doi.org/10.3389/fimmu.2022.804987.s007 https://figshare.com/articles/dataset/Table_4_Interacting_Effects_of_Sea_Louse_Lepeophtheirus_salmonis_Infection_and_Formalin-Killed_Aeromonas_salmonicida_on_Atlantic_Salmon_Skin_Transcriptome_xlsx/19407848 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_relation |
doi:10.3389/fimmu.2022.804987.s007 https://figshare.com/articles/dataset/Table_4_Interacting_Effects_of_Sea_Louse_Lepeophtheirus_salmonis_Infection_and_Formalin-Killed_Aeromonas_salmonicida_on_Atlantic_Salmon_Skin_Transcriptome_xlsx/19407848 |
op_rights |
CC BY 4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fimmu.2022.804987.s007 |
_version_ |
1766361963038769152 |