Table_1_Diversity and Effect of Increasing Temperature on the Activity of Methanotrophs in Sediments of Fildes Peninsula Freshwater Lakes, King George Island, Antarctica.XLSX
Global warming has a strong impact on polar regions. Particularly, the Antarctic Peninsula and nearby islands have experienced a marked warming trend in the past 50 years. Therefore, higher methane (CH 4 ) emissions from this area could be expected in the future. Since mitigation of these emissions...
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ftfrontimediafig:oai:figshare.com:article/19374455 2023-05-15T13:45:24+02:00 Table_1_Diversity and Effect of Increasing Temperature on the Activity of Methanotrophs in Sediments of Fildes Peninsula Freshwater Lakes, King George Island, Antarctica.XLSX Diego M. Roldán Daniel Carrizo Laura Sánchez-García Rodolfo Javier Menes 2022-03-17T05:29:14Z https://doi.org/10.3389/fmicb.2022.822552.s002 https://figshare.com/articles/dataset/Table_1_Diversity_and_Effect_of_Increasing_Temperature_on_the_Activity_of_Methanotrophs_in_Sediments_of_Fildes_Peninsula_Freshwater_Lakes_King_George_Island_Antarctica_XLSX/19374455 unknown doi:10.3389/fmicb.2022.822552.s002 https://figshare.com/articles/dataset/Table_1_Diversity_and_Effect_of_Increasing_Temperature_on_the_Activity_of_Methanotrophs_in_Sediments_of_Fildes_Peninsula_Freshwater_Lakes_King_George_Island_Antarctica_XLSX/19374455 CC BY 4.0 CC-BY Microbiology Microbial Genetics Microbial Ecology Mycology Antarctica global warming methanotrophs methane methane-oxidizing bacteria methanogenesis polar lakes Dataset 2022 ftfrontimediafig https://doi.org/10.3389/fmicb.2022.822552.s002 2022-03-24T00:08:07Z Global warming has a strong impact on polar regions. Particularly, the Antarctic Peninsula and nearby islands have experienced a marked warming trend in the past 50 years. Therefore, higher methane (CH 4 ) emissions from this area could be expected in the future. Since mitigation of these emissions can be carried out by microbial oxidation, understanding this biological process is crucial since to our knowledge, no related studies have been performed in this area before. In this work, the aerobic CH 4 oxidation potential of five freshwater lake sediments of Fildes Peninsula (King George Island, South Shetland Islands) was determined with values from 0.07 to 10 μmol CH 4 gdw –1 day –1 and revealed up to 100-fold increase in temperature gradients (5, 10, 15, and 20°C). The structure and diversity of the bacterial community in the sediments were analyzed by next-generation sequencing (Illumina MiSeq) of 16S rRNA and pmoA genes. A total of 4,836 ASVs were identified being Proteobacteria, Actinobacteriota, Acidobacteriota, and Bacteroidota the most abundant phyla. The analysis of the pmoA gene identified 200 ASVs of methanotrophs, being Methylobacter Clade 2 (Type I, family Methylococcaceae) the main responsible of the aerobic CH 4 oxidation. Moreover, both approaches revealed the presence of methanotrophs of the classes Gammaproteobacteria (families Methylococcaceae and Crenotrichaceae), Alphaproteobacteria (family Methylocystaceae), Verrucomicrobia (family Methylacidiphilaceae), and the candidate phylum of anaerobic methanotrophs Methylomirabilota. In addition, bacterial phospholipid fatty acids (PLFA) biomarkers were studied as a proxy for aerobic methane-oxidizing bacteria and confirmed these results. Methanotrophic bacterial diversity was significantly correlated with pH. In conclusion, our findings suggest that aerobic methanotrophs could mitigate in situ CH 4 emissions in a future scenario with higher temperatures in this climate-sensitive area. This study provides new insights into the diversity of ... Dataset Antarc* Antarctic Antarctic Peninsula Antarctica King George Island South Shetland Islands Frontiers: Figshare Antarctic The Antarctic Antarctic Peninsula King George Island South Shetland Islands Fildes ENVELOPE(-58.817,-58.817,-62.217,-62.217) Fildes peninsula ENVELOPE(-58.948,-58.948,-62.182,-62.182) |
institution |
Open Polar |
collection |
Frontiers: Figshare |
op_collection_id |
ftfrontimediafig |
language |
unknown |
topic |
Microbiology Microbial Genetics Microbial Ecology Mycology Antarctica global warming methanotrophs methane methane-oxidizing bacteria methanogenesis polar lakes |
spellingShingle |
Microbiology Microbial Genetics Microbial Ecology Mycology Antarctica global warming methanotrophs methane methane-oxidizing bacteria methanogenesis polar lakes Diego M. Roldán Daniel Carrizo Laura Sánchez-García Rodolfo Javier Menes Table_1_Diversity and Effect of Increasing Temperature on the Activity of Methanotrophs in Sediments of Fildes Peninsula Freshwater Lakes, King George Island, Antarctica.XLSX |
topic_facet |
Microbiology Microbial Genetics Microbial Ecology Mycology Antarctica global warming methanotrophs methane methane-oxidizing bacteria methanogenesis polar lakes |
description |
Global warming has a strong impact on polar regions. Particularly, the Antarctic Peninsula and nearby islands have experienced a marked warming trend in the past 50 years. Therefore, higher methane (CH 4 ) emissions from this area could be expected in the future. Since mitigation of these emissions can be carried out by microbial oxidation, understanding this biological process is crucial since to our knowledge, no related studies have been performed in this area before. In this work, the aerobic CH 4 oxidation potential of five freshwater lake sediments of Fildes Peninsula (King George Island, South Shetland Islands) was determined with values from 0.07 to 10 μmol CH 4 gdw –1 day –1 and revealed up to 100-fold increase in temperature gradients (5, 10, 15, and 20°C). The structure and diversity of the bacterial community in the sediments were analyzed by next-generation sequencing (Illumina MiSeq) of 16S rRNA and pmoA genes. A total of 4,836 ASVs were identified being Proteobacteria, Actinobacteriota, Acidobacteriota, and Bacteroidota the most abundant phyla. The analysis of the pmoA gene identified 200 ASVs of methanotrophs, being Methylobacter Clade 2 (Type I, family Methylococcaceae) the main responsible of the aerobic CH 4 oxidation. Moreover, both approaches revealed the presence of methanotrophs of the classes Gammaproteobacteria (families Methylococcaceae and Crenotrichaceae), Alphaproteobacteria (family Methylocystaceae), Verrucomicrobia (family Methylacidiphilaceae), and the candidate phylum of anaerobic methanotrophs Methylomirabilota. In addition, bacterial phospholipid fatty acids (PLFA) biomarkers were studied as a proxy for aerobic methane-oxidizing bacteria and confirmed these results. Methanotrophic bacterial diversity was significantly correlated with pH. In conclusion, our findings suggest that aerobic methanotrophs could mitigate in situ CH 4 emissions in a future scenario with higher temperatures in this climate-sensitive area. This study provides new insights into the diversity of ... |
format |
Dataset |
author |
Diego M. Roldán Daniel Carrizo Laura Sánchez-García Rodolfo Javier Menes |
author_facet |
Diego M. Roldán Daniel Carrizo Laura Sánchez-García Rodolfo Javier Menes |
author_sort |
Diego M. Roldán |
title |
Table_1_Diversity and Effect of Increasing Temperature on the Activity of Methanotrophs in Sediments of Fildes Peninsula Freshwater Lakes, King George Island, Antarctica.XLSX |
title_short |
Table_1_Diversity and Effect of Increasing Temperature on the Activity of Methanotrophs in Sediments of Fildes Peninsula Freshwater Lakes, King George Island, Antarctica.XLSX |
title_full |
Table_1_Diversity and Effect of Increasing Temperature on the Activity of Methanotrophs in Sediments of Fildes Peninsula Freshwater Lakes, King George Island, Antarctica.XLSX |
title_fullStr |
Table_1_Diversity and Effect of Increasing Temperature on the Activity of Methanotrophs in Sediments of Fildes Peninsula Freshwater Lakes, King George Island, Antarctica.XLSX |
title_full_unstemmed |
Table_1_Diversity and Effect of Increasing Temperature on the Activity of Methanotrophs in Sediments of Fildes Peninsula Freshwater Lakes, King George Island, Antarctica.XLSX |
title_sort |
table_1_diversity and effect of increasing temperature on the activity of methanotrophs in sediments of fildes peninsula freshwater lakes, king george island, antarctica.xlsx |
publishDate |
2022 |
url |
https://doi.org/10.3389/fmicb.2022.822552.s002 https://figshare.com/articles/dataset/Table_1_Diversity_and_Effect_of_Increasing_Temperature_on_the_Activity_of_Methanotrophs_in_Sediments_of_Fildes_Peninsula_Freshwater_Lakes_King_George_Island_Antarctica_XLSX/19374455 |
long_lat |
ENVELOPE(-58.817,-58.817,-62.217,-62.217) ENVELOPE(-58.948,-58.948,-62.182,-62.182) |
geographic |
Antarctic The Antarctic Antarctic Peninsula King George Island South Shetland Islands Fildes Fildes peninsula |
geographic_facet |
Antarctic The Antarctic Antarctic Peninsula King George Island South Shetland Islands Fildes Fildes peninsula |
genre |
Antarc* Antarctic Antarctic Peninsula Antarctica King George Island South Shetland Islands |
genre_facet |
Antarc* Antarctic Antarctic Peninsula Antarctica King George Island South Shetland Islands |
op_relation |
doi:10.3389/fmicb.2022.822552.s002 https://figshare.com/articles/dataset/Table_1_Diversity_and_Effect_of_Increasing_Temperature_on_the_Activity_of_Methanotrophs_in_Sediments_of_Fildes_Peninsula_Freshwater_Lakes_King_George_Island_Antarctica_XLSX/19374455 |
op_rights |
CC BY 4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fmicb.2022.822552.s002 |
_version_ |
1766223575133454336 |