Data_Sheet_1_Impact of Lhcx2 on Acclimation to Low Iron Conditions in the Diatom Phaeodactylum tricornutum.PDF
Iron is a cofactor of photosystems and electron carriers in the photosynthetic electron transport chain. Low concentrations of dissolved iron are, therefore, the predominant factor that limits the growth of phototrophs in large parts of the open sea like the Southern Ocean and the North Pacific, res...
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ftfrontimediafig:oai:figshare.com:article/19369388 2023-05-15T18:25:52+02:00 Data_Sheet_1_Impact of Lhcx2 on Acclimation to Low Iron Conditions in the Diatom Phaeodactylum tricornutum.PDF Jochen M. Buck Marie Wünsch Alexander F. Schober Peter G. Kroth Bernard Lepetit 2022-03-16T16:27:32Z https://doi.org/10.3389/fpls.2022.841058.s001 https://figshare.com/articles/dataset/Data_Sheet_1_Impact_of_Lhcx2_on_Acclimation_to_Low_Iron_Conditions_in_the_Diatom_Phaeodactylum_tricornutum_PDF/19369388 unknown doi:10.3389/fpls.2022.841058.s001 https://figshare.com/articles/dataset/Data_Sheet_1_Impact_of_Lhcx2_on_Acclimation_to_Low_Iron_Conditions_in_the_Diatom_Phaeodactylum_tricornutum_PDF/19369388 CC BY 4.0 CC-BY Botany Plant Biology Plant Systematics and Taxonomy Plant Cell and Molecular Biology Plant Developmental and Reproductive Biology Plant Pathology Plant Physiology Plant Biology not elsewhere classified diatoms Phaeodactylum tricornutum iron NPQ photoprotection Lhcx reactive oxygen species photosystem Dataset 2022 ftfrontimediafig https://doi.org/10.3389/fpls.2022.841058.s001 2022-03-17T00:02:32Z Iron is a cofactor of photosystems and electron carriers in the photosynthetic electron transport chain. Low concentrations of dissolved iron are, therefore, the predominant factor that limits the growth of phototrophs in large parts of the open sea like the Southern Ocean and the North Pacific, resulting in “high nutrient–low chlorophyll” (HNLC) areas. Diatoms are among the most abundant microalgae in HNLC zones. Besides efficient iron uptake mechanisms, efficient photoprotection might be one of the key traits enabling them to outcompete other algae in HNLC regions. In diatoms, Lhcx proteins play a crucial role in one of the main photoprotective mechanisms, the energy-dependent fluorescence quenching (qE). The expression of Lhcx proteins is strongly influenced by various environmental triggers. We show that Lhcx2 responds specifically and in a very sensitive manner to iron limitation in the diatom Phaeodactylum tricornutum on the same timescale as the known iron-regulated genes ISIP1 and CCHH11. By comparing Lhcx2 knockout lines with wild type cells, we reveal that a strongly increased qE under iron limitation is based on the upregulation of Lhcx2. Other observed iron acclimation phenotypes in P. tricornutum include a massively reduced chlorophyll a content/cell, a changed ratio of light harvesting and photoprotective pigments per chlorophyll a, a decreased amount of photosystem II and photosystem I cores, an increased functional photosystem II absorption cross section, and decoupled antenna complexes. H 2 O 2 formation at photosystem I induced by high light is lowered in iron-limited cells, while the amount of total reactive oxygen species is rather increased. Our data indicate a possible reduction in singlet oxygen by Lhcx2-based qE, while the other iron acclimation phenotype parameters monitored are not affected by the amount of Lhcx2 and qE. Dataset Southern Ocean Frontiers: Figshare Southern Ocean Pacific |
institution |
Open Polar |
collection |
Frontiers: Figshare |
op_collection_id |
ftfrontimediafig |
language |
unknown |
topic |
Botany Plant Biology Plant Systematics and Taxonomy Plant Cell and Molecular Biology Plant Developmental and Reproductive Biology Plant Pathology Plant Physiology Plant Biology not elsewhere classified diatoms Phaeodactylum tricornutum iron NPQ photoprotection Lhcx reactive oxygen species photosystem |
spellingShingle |
Botany Plant Biology Plant Systematics and Taxonomy Plant Cell and Molecular Biology Plant Developmental and Reproductive Biology Plant Pathology Plant Physiology Plant Biology not elsewhere classified diatoms Phaeodactylum tricornutum iron NPQ photoprotection Lhcx reactive oxygen species photosystem Jochen M. Buck Marie Wünsch Alexander F. Schober Peter G. Kroth Bernard Lepetit Data_Sheet_1_Impact of Lhcx2 on Acclimation to Low Iron Conditions in the Diatom Phaeodactylum tricornutum.PDF |
topic_facet |
Botany Plant Biology Plant Systematics and Taxonomy Plant Cell and Molecular Biology Plant Developmental and Reproductive Biology Plant Pathology Plant Physiology Plant Biology not elsewhere classified diatoms Phaeodactylum tricornutum iron NPQ photoprotection Lhcx reactive oxygen species photosystem |
description |
Iron is a cofactor of photosystems and electron carriers in the photosynthetic electron transport chain. Low concentrations of dissolved iron are, therefore, the predominant factor that limits the growth of phototrophs in large parts of the open sea like the Southern Ocean and the North Pacific, resulting in “high nutrient–low chlorophyll” (HNLC) areas. Diatoms are among the most abundant microalgae in HNLC zones. Besides efficient iron uptake mechanisms, efficient photoprotection might be one of the key traits enabling them to outcompete other algae in HNLC regions. In diatoms, Lhcx proteins play a crucial role in one of the main photoprotective mechanisms, the energy-dependent fluorescence quenching (qE). The expression of Lhcx proteins is strongly influenced by various environmental triggers. We show that Lhcx2 responds specifically and in a very sensitive manner to iron limitation in the diatom Phaeodactylum tricornutum on the same timescale as the known iron-regulated genes ISIP1 and CCHH11. By comparing Lhcx2 knockout lines with wild type cells, we reveal that a strongly increased qE under iron limitation is based on the upregulation of Lhcx2. Other observed iron acclimation phenotypes in P. tricornutum include a massively reduced chlorophyll a content/cell, a changed ratio of light harvesting and photoprotective pigments per chlorophyll a, a decreased amount of photosystem II and photosystem I cores, an increased functional photosystem II absorption cross section, and decoupled antenna complexes. H 2 O 2 formation at photosystem I induced by high light is lowered in iron-limited cells, while the amount of total reactive oxygen species is rather increased. Our data indicate a possible reduction in singlet oxygen by Lhcx2-based qE, while the other iron acclimation phenotype parameters monitored are not affected by the amount of Lhcx2 and qE. |
format |
Dataset |
author |
Jochen M. Buck Marie Wünsch Alexander F. Schober Peter G. Kroth Bernard Lepetit |
author_facet |
Jochen M. Buck Marie Wünsch Alexander F. Schober Peter G. Kroth Bernard Lepetit |
author_sort |
Jochen M. Buck |
title |
Data_Sheet_1_Impact of Lhcx2 on Acclimation to Low Iron Conditions in the Diatom Phaeodactylum tricornutum.PDF |
title_short |
Data_Sheet_1_Impact of Lhcx2 on Acclimation to Low Iron Conditions in the Diatom Phaeodactylum tricornutum.PDF |
title_full |
Data_Sheet_1_Impact of Lhcx2 on Acclimation to Low Iron Conditions in the Diatom Phaeodactylum tricornutum.PDF |
title_fullStr |
Data_Sheet_1_Impact of Lhcx2 on Acclimation to Low Iron Conditions in the Diatom Phaeodactylum tricornutum.PDF |
title_full_unstemmed |
Data_Sheet_1_Impact of Lhcx2 on Acclimation to Low Iron Conditions in the Diatom Phaeodactylum tricornutum.PDF |
title_sort |
data_sheet_1_impact of lhcx2 on acclimation to low iron conditions in the diatom phaeodactylum tricornutum.pdf |
publishDate |
2022 |
url |
https://doi.org/10.3389/fpls.2022.841058.s001 https://figshare.com/articles/dataset/Data_Sheet_1_Impact_of_Lhcx2_on_Acclimation_to_Low_Iron_Conditions_in_the_Diatom_Phaeodactylum_tricornutum_PDF/19369388 |
geographic |
Southern Ocean Pacific |
geographic_facet |
Southern Ocean Pacific |
genre |
Southern Ocean |
genre_facet |
Southern Ocean |
op_relation |
doi:10.3389/fpls.2022.841058.s001 https://figshare.com/articles/dataset/Data_Sheet_1_Impact_of_Lhcx2_on_Acclimation_to_Low_Iron_Conditions_in_the_Diatom_Phaeodactylum_tricornutum_PDF/19369388 |
op_rights |
CC BY 4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fpls.2022.841058.s001 |
_version_ |
1766207562273783808 |