Table_1_Characteristics of Whale Müller Glia in Primary and Immortalized Cultures.DOCX

• Main Authors: Xandra Pereiro, Sandra Beriain, Lara Rodriguez, David Roiz-Valle, Noelia Ruzafa, Elena Vecino
• Format: Dataset
• Language: unknown
• Published: 2022
• Subjects: Neuroscience; Biological Engineering; Developmental Biology; Stem Cells; Artificial Intelligence and Image Processing; Endocrinology; Radiology and Organ Imaging; Autonomic Nervous System; Cellular Nervous System; Central Nervous System; Sensory Systems; Clinical Nursing: Tertiary (Rehabilitative); Decision Making; Rehabilitation Engineering; Biomedical Engineering not elsewhere classified; Signal Processing; Neurogenetics; Image Processing; Müller cells; glia; retina; whale; immortalization; cell line; Balaenoptera borealis; Sei Whale
• Online Access: https://doi.org/10.3389/fnins.2022.854278.s002; https://figshare.com/articles/dataset/Table_1_Characteristics_of_Whale_M_ller_Glia_in_Primary_and_Immortalized_Cultures_DOCX/19353389

Müller cells are the principal glial cells in the retina and they assume many of the functions carried out by astrocytes, oligodendrocytes and ependymal cells in other regions of the central nervous system. Müller cells express growth factors, neurotransmitter transporters and antioxidant agents that could fulfill important roles in preventing excitotoxic damage to retinal neurons. Vertebrate Müller cells are well-defined cells, characterized by a common set of features throughout the phylum. Nevertheless, several major differences have been observed among the Müller cells in distinct vertebrates, such as neurogenesis, the capacity to reprogram fish Müller glia to neurons. Here, the Müller glia of the largest adult mammal in the world, the whale, have been analyzed, and given the difficulties in obtaining cetacean cells for study, these whale glia were analyzed both in primary cultures and as immortalized whale Müller cells. After isolating the retina from the eye of a beached sei whale (Balaenoptera borealis), primary Müller cell cultures were established and once the cultures reached confluence, half of the cultures were immortalized with the simian virus 40 (SV40) large T-antigen commonly used to immortalize human cell lines. The primary cell cultures were grown until cells reached senescence. Expression of the principal molecular markers of Müller cells (GFAP, Vimentin and Glutamine synthetase) was studied in both primary and immortalized cells at each culture passage. Proliferation kinetics of the cells were analyzed by time-lapse microscopy: the time between divisions, the time that cells take to divide, and the proportion of dividing cells in the same field. The karyotypes of the primary and immortalized whale Müller cells were also characterized. Our results shown that W21M proliferate more rapidly and they have a stable karyotype. W21M cells display a heterogeneous cell morphology, less motility and a distinctive expression of some typical molecular markers of Müller cells, with an increase in ...