Table4_Investigating the Functional Role of Hypothetical Proteins From an Antarctic Bacterium Pseudomonas sp. Lz4W: Emphasis on Identifying Proteins Involved in Cold Adaptation.xlsx
Exploring the molecular mechanisms behind bacterial adaptation to extreme temperatures has potential biotechnological applications. In the present study, Pseudomonas sp. Lz4W, a Gram-negative psychrophilic bacterium adapted to survive in Antarctica, was selected to decipher the molecular mechanism u...
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ftfrontimediafig:oai:figshare.com:article/19345517 2023-05-15T13:45:24+02:00 Table4_Investigating the Functional Role of Hypothetical Proteins From an Antarctic Bacterium Pseudomonas sp. Lz4W: Emphasis on Identifying Proteins Involved in Cold Adaptation.xlsx Johny Ijaq Deepika Chandra Malay Kumar Ray M. V. Jagannadham 2022-03-11T12:33:20Z https://doi.org/10.3389/fgene.2022.825269.s006 https://figshare.com/articles/dataset/Table4_Investigating_the_Functional_Role_of_Hypothetical_Proteins_From_an_Antarctic_Bacterium_Pseudomonas_sp_Lz4W_Emphasis_on_Identifying_Proteins_Involved_in_Cold_Adaptation_xlsx/19345517 unknown doi:10.3389/fgene.2022.825269.s006 https://figshare.com/articles/dataset/Table4_Investigating_the_Functional_Role_of_Hypothetical_Proteins_From_an_Antarctic_Bacterium_Pseudomonas_sp_Lz4W_Emphasis_on_Identifying_Proteins_Involved_in_Cold_Adaptation_xlsx/19345517 CC BY 4.0 CC-BY Genetics Genetic Engineering Biomarkers Developmental Genetics (incl. Sex Determination) Epigenetics (incl. Genome Methylation and Epigenomics) Gene Expression (incl. Microarray and other genome-wide approaches) Genome Structure and Regulation Genomics Genetically Modified Animals Livestock Cloning Gene and Molecular Therapy Pseudomonas sp. Lz4W LC-MS/MS hypothetical proteins cold adaptation functional annotation Dataset 2022 ftfrontimediafig https://doi.org/10.3389/fgene.2022.825269.s006 2022-03-17T00:06:44Z Exploring the molecular mechanisms behind bacterial adaptation to extreme temperatures has potential biotechnological applications. In the present study, Pseudomonas sp. Lz4W, a Gram-negative psychrophilic bacterium adapted to survive in Antarctica, was selected to decipher the molecular mechanism underlying the cold adaptation. Proteome analysis of the isolates grown at 4°C was performed to identify the proteins and pathways that are responsible for the adaptation. However, many proteins from the expressed proteome were found to be hypothetical proteins (HPs), whose function is unknown. Investigating the functional roles of these proteins may provide additional information in the biological understanding of the bacterial cold adaptation. Thus, our study aimed to assign functions to these HPs and understand their role at the molecular level. We used a structured insilico workflow combining different bioinformatics tools and databases for functional annotation. Pseudomonas sp. Lz4W genome (CP017432, version 1) contains 4493 genes and 4412 coding sequences (CDS), of which 743 CDS were annotated as HPs. Of these, from the proteome analysis, 61 HPs were found to be expressed consistently at the protein level. The amino acid sequences of these 61 HPs were submitted to our workflow and we could successfully assign a function to 18 HPs. Most of these proteins were predicted to be involved in biological mechanisms of cold adaptations such as peptidoglycan metabolism, cell wall organization, ATP hydrolysis, outer membrane fluidity, catalysis, and others. This study provided a better understanding of the functional significance of HPs in cold adaptation of Pseudomonas sp. Lz4W. Our approach emphasizes the importance of addressing the “hypothetical protein problem” for a thorough understanding of mechanisms at the cellular level, as well as, provided the assessment of integrating proteomics methods with various annotation and curation approaches to characterize hypothetical or uncharacterized protein data. The MS ... Dataset Antarc* Antarctic Antarctica Frontiers: Figshare Antarctic |
institution |
Open Polar |
collection |
Frontiers: Figshare |
op_collection_id |
ftfrontimediafig |
language |
unknown |
topic |
Genetics Genetic Engineering Biomarkers Developmental Genetics (incl. Sex Determination) Epigenetics (incl. Genome Methylation and Epigenomics) Gene Expression (incl. Microarray and other genome-wide approaches) Genome Structure and Regulation Genomics Genetically Modified Animals Livestock Cloning Gene and Molecular Therapy Pseudomonas sp. Lz4W LC-MS/MS hypothetical proteins cold adaptation functional annotation |
spellingShingle |
Genetics Genetic Engineering Biomarkers Developmental Genetics (incl. Sex Determination) Epigenetics (incl. Genome Methylation and Epigenomics) Gene Expression (incl. Microarray and other genome-wide approaches) Genome Structure and Regulation Genomics Genetically Modified Animals Livestock Cloning Gene and Molecular Therapy Pseudomonas sp. Lz4W LC-MS/MS hypothetical proteins cold adaptation functional annotation Johny Ijaq Deepika Chandra Malay Kumar Ray M. V. Jagannadham Table4_Investigating the Functional Role of Hypothetical Proteins From an Antarctic Bacterium Pseudomonas sp. Lz4W: Emphasis on Identifying Proteins Involved in Cold Adaptation.xlsx |
topic_facet |
Genetics Genetic Engineering Biomarkers Developmental Genetics (incl. Sex Determination) Epigenetics (incl. Genome Methylation and Epigenomics) Gene Expression (incl. Microarray and other genome-wide approaches) Genome Structure and Regulation Genomics Genetically Modified Animals Livestock Cloning Gene and Molecular Therapy Pseudomonas sp. Lz4W LC-MS/MS hypothetical proteins cold adaptation functional annotation |
description |
Exploring the molecular mechanisms behind bacterial adaptation to extreme temperatures has potential biotechnological applications. In the present study, Pseudomonas sp. Lz4W, a Gram-negative psychrophilic bacterium adapted to survive in Antarctica, was selected to decipher the molecular mechanism underlying the cold adaptation. Proteome analysis of the isolates grown at 4°C was performed to identify the proteins and pathways that are responsible for the adaptation. However, many proteins from the expressed proteome were found to be hypothetical proteins (HPs), whose function is unknown. Investigating the functional roles of these proteins may provide additional information in the biological understanding of the bacterial cold adaptation. Thus, our study aimed to assign functions to these HPs and understand their role at the molecular level. We used a structured insilico workflow combining different bioinformatics tools and databases for functional annotation. Pseudomonas sp. Lz4W genome (CP017432, version 1) contains 4493 genes and 4412 coding sequences (CDS), of which 743 CDS were annotated as HPs. Of these, from the proteome analysis, 61 HPs were found to be expressed consistently at the protein level. The amino acid sequences of these 61 HPs were submitted to our workflow and we could successfully assign a function to 18 HPs. Most of these proteins were predicted to be involved in biological mechanisms of cold adaptations such as peptidoglycan metabolism, cell wall organization, ATP hydrolysis, outer membrane fluidity, catalysis, and others. This study provided a better understanding of the functional significance of HPs in cold adaptation of Pseudomonas sp. Lz4W. Our approach emphasizes the importance of addressing the “hypothetical protein problem” for a thorough understanding of mechanisms at the cellular level, as well as, provided the assessment of integrating proteomics methods with various annotation and curation approaches to characterize hypothetical or uncharacterized protein data. The MS ... |
format |
Dataset |
author |
Johny Ijaq Deepika Chandra Malay Kumar Ray M. V. Jagannadham |
author_facet |
Johny Ijaq Deepika Chandra Malay Kumar Ray M. V. Jagannadham |
author_sort |
Johny Ijaq |
title |
Table4_Investigating the Functional Role of Hypothetical Proteins From an Antarctic Bacterium Pseudomonas sp. Lz4W: Emphasis on Identifying Proteins Involved in Cold Adaptation.xlsx |
title_short |
Table4_Investigating the Functional Role of Hypothetical Proteins From an Antarctic Bacterium Pseudomonas sp. Lz4W: Emphasis on Identifying Proteins Involved in Cold Adaptation.xlsx |
title_full |
Table4_Investigating the Functional Role of Hypothetical Proteins From an Antarctic Bacterium Pseudomonas sp. Lz4W: Emphasis on Identifying Proteins Involved in Cold Adaptation.xlsx |
title_fullStr |
Table4_Investigating the Functional Role of Hypothetical Proteins From an Antarctic Bacterium Pseudomonas sp. Lz4W: Emphasis on Identifying Proteins Involved in Cold Adaptation.xlsx |
title_full_unstemmed |
Table4_Investigating the Functional Role of Hypothetical Proteins From an Antarctic Bacterium Pseudomonas sp. Lz4W: Emphasis on Identifying Proteins Involved in Cold Adaptation.xlsx |
title_sort |
table4_investigating the functional role of hypothetical proteins from an antarctic bacterium pseudomonas sp. lz4w: emphasis on identifying proteins involved in cold adaptation.xlsx |
publishDate |
2022 |
url |
https://doi.org/10.3389/fgene.2022.825269.s006 https://figshare.com/articles/dataset/Table4_Investigating_the_Functional_Role_of_Hypothetical_Proteins_From_an_Antarctic_Bacterium_Pseudomonas_sp_Lz4W_Emphasis_on_Identifying_Proteins_Involved_in_Cold_Adaptation_xlsx/19345517 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic Antarctica |
genre_facet |
Antarc* Antarctic Antarctica |
op_relation |
doi:10.3389/fgene.2022.825269.s006 https://figshare.com/articles/dataset/Table4_Investigating_the_Functional_Role_of_Hypothetical_Proteins_From_an_Antarctic_Bacterium_Pseudomonas_sp_Lz4W_Emphasis_on_Identifying_Proteins_Involved_in_Cold_Adaptation_xlsx/19345517 |
op_rights |
CC BY 4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fgene.2022.825269.s006 |
_version_ |
1766223549390913536 |